p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells
An effective regulation of quiescence plays a key role in the differentiation, plasticity, and prevention of stem cells from becoming malignant. The state of quiescence is being controlled by the pRb family proteins which show overlapping functions in cell cycle regulation; however, their roles in c...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8883436 |
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| _version_ | 1832566373511856128 |
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| author | Boris Popov Nikolai Petrov Vladimir Ryabov Igor Evsyukov |
| author_facet | Boris Popov Nikolai Petrov Vladimir Ryabov Igor Evsyukov |
| author_sort | Boris Popov |
| collection | DOAJ |
| description | An effective regulation of quiescence plays a key role in the differentiation, plasticity, and prevention of stem cells from becoming malignant. The state of quiescence is being controlled by the pRb family proteins which show overlapping functions in cell cycle regulation; however, their roles in controlling the proliferation of mesenchymal stem cells (MSCs) remain to be understood. This study investigated the regulation of transient quiescence using growth curves, proliferation assay, the cytometric evaluation of cell cycle, Western blotting, and the electromobility gel shift assay (EMSA) on synchronized MSCs of the C3H10Т1/2 and control cells with different statuses of pRb proteins. It has been found that functional steady-state level of p130 but not pRb plays a critical role for entering, exiting, and maintenance of transient quiescence in multipotent mesenchymal stem cells. |
| format | Article |
| id | doaj-art-e2a9fe41fa594295844bea9fabbb2895 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e2a9fe41fa594295844bea9fabbb28952025-02-03T01:04:23ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88834368883436p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem CellsBoris Popov0Nikolai Petrov1Vladimir Ryabov2Igor Evsyukov3Institute of Cytology Russian Academy of Sciences, St. Petersburg 194064, RussiaInstitute of Cytology Russian Academy of Sciences, St. Petersburg 194064, RussiaInstitute of Cytology Russian Academy of Sciences, St. Petersburg 194064, RussiaInstitute of Cytology Russian Academy of Sciences, St. Petersburg 194064, RussiaAn effective regulation of quiescence plays a key role in the differentiation, plasticity, and prevention of stem cells from becoming malignant. The state of quiescence is being controlled by the pRb family proteins which show overlapping functions in cell cycle regulation; however, their roles in controlling the proliferation of mesenchymal stem cells (MSCs) remain to be understood. This study investigated the regulation of transient quiescence using growth curves, proliferation assay, the cytometric evaluation of cell cycle, Western blotting, and the electromobility gel shift assay (EMSA) on synchronized MSCs of the C3H10Т1/2 and control cells with different statuses of pRb proteins. It has been found that functional steady-state level of p130 but not pRb plays a critical role for entering, exiting, and maintenance of transient quiescence in multipotent mesenchymal stem cells.http://dx.doi.org/10.1155/2020/8883436 |
| spellingShingle | Boris Popov Nikolai Petrov Vladimir Ryabov Igor Evsyukov p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells Stem Cells International |
| title | p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells |
| title_full | p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells |
| title_fullStr | p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells |
| title_full_unstemmed | p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells |
| title_short | p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells |
| title_sort | p130 and prb in the maintenance of transient quiescence of mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2020/8883436 |
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