Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program
Genetic diversity present in gene pool is an important determination for breeding programs, and characterization is useful of building crop plant collections primarily based on the knowledge of the presence of valuable genes and traits. Developing successful varieties for increasing the future yield...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hasan Eleroğlu
2014-10-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/249 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849470116754882560 |
|---|---|
| author | Muhammad Azhar Shah Azeem Iqbal Khan Faisal Saeed Awan Hafeez Ahmed Sadaqat Sultan Bahadur Aamir Rasheed Faheem Shehzad Baloch |
| author_facet | Muhammad Azhar Shah Azeem Iqbal Khan Faisal Saeed Awan Hafeez Ahmed Sadaqat Sultan Bahadur Aamir Rasheed Faheem Shehzad Baloch |
| author_sort | Muhammad Azhar Shah |
| collection | DOAJ |
| description | Genetic diversity present in gene pool is an important determination for breeding programs, and characterization is useful of building crop plant collections primarily based on the knowledge of the presence of valuable genes and traits. Developing successful varieties for increasing the future yield and quality of tomato depend mainly on the genetic diversity of parents used in the breeding program. Molecular characterization of 21 tomato genotypes used in in Pakistani breeding program was studied using random amplified polymorphic DNA (RAPD) markers. Total 102 bands were amplified among 21 genotypes using 20 RAPD primers. Overall 73.5% polymorphism was shown as 75 out of 102 loci were polymorphic. High degree of divergence between varieties was indicated by low level of monomorphic bands. The number of PCR products per primer varied from 2-8 with an average of 5.1 bands per primer. Primer GL J-20 and GL C-09 produced maximum number of bands whereas the primers GL A-09 produced the lowest. The polymorphism per RAPD primer ranged from 50% to 100% with an average of 73.5%. The accumulative analysis of amplified products generated by RAPD’s was enough to assess the genetic diversity among the genotypes. The information would be helpful for formulating future breeding and genome mapping programs. This study will also work as an indicator for tomato breeders to evolve varieties with genetic diverse back ground to achieve sustainability in tomato production in the country. |
| format | Article |
| id | doaj-art-e2a8077c083d46f28b82c6678303fe30 |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2014-10-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-e2a8077c083d46f28b82c6678303fe302025-08-20T03:25:15ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2014-10-013312613210.24925/turjaf.v3i3.126-132.249106Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding ProgramMuhammad Azhar Shah0Azeem Iqbal Khan1Faisal Saeed Awan2Hafeez Ahmed Sadaqat3Sultan Bahadur4Aamir Rasheed5Faheem Shehzad Baloch6University of Agriculture, Faisalabad, Centre of Agricultural Biochemistry and Biotechnology (CABB)University of Agriculture, Faisalabad, Centre of Agricultural Biochemistry and Biotechnology (CABB)University of Agriculture, Faisalabad, Centre of Agricultural Biochemistry and Biotechnology (CABB)University of Agriculture, Faisalabad, Department of Plant Breeding and GeneticsNursery Division, Desert Group, DubaiDepartment of Plant Breeding and Genetics, Faculty of Agricultural Sciences and Texchnologies, Bahuddin Zakariya University, MultanNigde UniversityGenetic diversity present in gene pool is an important determination for breeding programs, and characterization is useful of building crop plant collections primarily based on the knowledge of the presence of valuable genes and traits. Developing successful varieties for increasing the future yield and quality of tomato depend mainly on the genetic diversity of parents used in the breeding program. Molecular characterization of 21 tomato genotypes used in in Pakistani breeding program was studied using random amplified polymorphic DNA (RAPD) markers. Total 102 bands were amplified among 21 genotypes using 20 RAPD primers. Overall 73.5% polymorphism was shown as 75 out of 102 loci were polymorphic. High degree of divergence between varieties was indicated by low level of monomorphic bands. The number of PCR products per primer varied from 2-8 with an average of 5.1 bands per primer. Primer GL J-20 and GL C-09 produced maximum number of bands whereas the primers GL A-09 produced the lowest. The polymorphism per RAPD primer ranged from 50% to 100% with an average of 73.5%. The accumulative analysis of amplified products generated by RAPD’s was enough to assess the genetic diversity among the genotypes. The information would be helpful for formulating future breeding and genome mapping programs. This study will also work as an indicator for tomato breeders to evolve varieties with genetic diverse back ground to achieve sustainability in tomato production in the country.http://www.agrifoodscience.com/index.php/TURJAF/article/view/249Genetic relationshipDiversityDNA markersPakistanTomato |
| spellingShingle | Muhammad Azhar Shah Azeem Iqbal Khan Faisal Saeed Awan Hafeez Ahmed Sadaqat Sultan Bahadur Aamir Rasheed Faheem Shehzad Baloch Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program Turkish Journal of Agriculture: Food Science and Technology Genetic relationship Diversity DNA markers Pakistan Tomato |
| title | Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program |
| title_full | Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program |
| title_fullStr | Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program |
| title_full_unstemmed | Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program |
| title_short | Genetic Diversity of Some Tomato Cultivars and Breeding Lines Commonly Used in Pakistani Breeding Program |
| title_sort | genetic diversity of some tomato cultivars and breeding lines commonly used in pakistani breeding program |
| topic | Genetic relationship Diversity DNA markers Pakistan Tomato |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/249 |
| work_keys_str_mv | AT muhammadazharshah geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram AT azeemiqbalkhan geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram AT faisalsaeedawan geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram AT hafeezahmedsadaqat geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram AT sultanbahadur geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram AT aamirrasheed geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram AT faheemshehzadbaloch geneticdiversityofsometomatocultivarsandbreedinglinescommonlyusedinpakistanibreedingprogram |