Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks
Duck plague (DP) is an acute, febrile, septicemic infectious disease caused by duck plague virus (DPV). pUL47, which is encoded by the DPV UL47 gene, is a relatively abundant late tegument protein in virions, and its effects on the life cycle and virulence of viruses remain unclear. Herein, we gener...
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Elsevier
2025-06-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125003311 |
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| author | Yuxi Cui Siyu Chen Mingshu Wang Wei Zhang Qiao Yang Xumin Ou Di Sun Yu He Bin Tian Zhen Wu Shaqiu Zhang Juan Huang Ying Wu Xinxin Zhao Dekang Zhu Shun Chen Mafeng Liu Renyong Jia Anchun Cheng |
| author_facet | Yuxi Cui Siyu Chen Mingshu Wang Wei Zhang Qiao Yang Xumin Ou Di Sun Yu He Bin Tian Zhen Wu Shaqiu Zhang Juan Huang Ying Wu Xinxin Zhao Dekang Zhu Shun Chen Mafeng Liu Renyong Jia Anchun Cheng |
| author_sort | Yuxi Cui |
| collection | DOAJ |
| description | Duck plague (DP) is an acute, febrile, septicemic infectious disease caused by duck plague virus (DPV). pUL47, which is encoded by the DPV UL47 gene, is a relatively abundant late tegument protein in virions, and its effects on the life cycle and virulence of viruses remain unclear. Herein, we generated UL47-deficient DPVs through a two-step Red recombination system and examined the effects of UL47 on the viral life cycle, virulence, and immune protection of UL47-deficient strains. The results showed that the deletion of the DPV UL47 gene affected the release and cell-to-cell spread of the virus, thereby reducing the efficiency of mature virion generation and further affecting the proliferation of the virus. The absence of the DPV UL47 gene significantly reduced the virulence of the virus but maintained immunogenicity and immune efficacy, thereby inducing the production of DPV-specific antibodies to resist strong attack by DP. These findings offer valuable insights into the functional characterization of the UL47 gene and the development of an attenuated vaccine based on this gene. Furthermore, this study provides a novel approach for the prevention and control of duck plague. |
| format | Article |
| id | doaj-art-e29a8190480c4471b7d82ca82d940379 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e29a8190480c4471b7d82ca82d9403792025-08-20T02:32:04ZengElsevierPoultry Science0032-57912025-06-01104610509210.1016/j.psj.2025.105092Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducksYuxi Cui0Siyu Chen1Mingshu Wang2Wei Zhang3Qiao Yang4Xumin Ou5Di Sun6Yu He7Bin Tian8Zhen Wu9Shaqiu Zhang10Juan Huang11Ying Wu12Xinxin Zhao13Dekang Zhu14Shun Chen15Mafeng Liu16Renyong Jia17Anchun Cheng18Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaSinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou 225100, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaEngineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Veterinary Immunology and Green Drugs, Veterinary Department in College of Aminal Science, State Key Laboratory of Green Pesticide, Guizhou University, Guiyang 550025, China; Corresponding author.Duck plague (DP) is an acute, febrile, septicemic infectious disease caused by duck plague virus (DPV). pUL47, which is encoded by the DPV UL47 gene, is a relatively abundant late tegument protein in virions, and its effects on the life cycle and virulence of viruses remain unclear. Herein, we generated UL47-deficient DPVs through a two-step Red recombination system and examined the effects of UL47 on the viral life cycle, virulence, and immune protection of UL47-deficient strains. The results showed that the deletion of the DPV UL47 gene affected the release and cell-to-cell spread of the virus, thereby reducing the efficiency of mature virion generation and further affecting the proliferation of the virus. The absence of the DPV UL47 gene significantly reduced the virulence of the virus but maintained immunogenicity and immune efficacy, thereby inducing the production of DPV-specific antibodies to resist strong attack by DP. These findings offer valuable insights into the functional characterization of the UL47 gene and the development of an attenuated vaccine based on this gene. Furthermore, this study provides a novel approach for the prevention and control of duck plague.http://www.sciencedirect.com/science/article/pii/S0032579125003311Duck plague virusUL47Life cycleVirulenceImmune protection |
| spellingShingle | Yuxi Cui Siyu Chen Mingshu Wang Wei Zhang Qiao Yang Xumin Ou Di Sun Yu He Bin Tian Zhen Wu Shaqiu Zhang Juan Huang Ying Wu Xinxin Zhao Dekang Zhu Shun Chen Mafeng Liu Renyong Jia Anchun Cheng Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks Poultry Science Duck plague virus UL47 Life cycle Virulence Immune protection |
| title | Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks |
| title_full | Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks |
| title_fullStr | Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks |
| title_full_unstemmed | Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks |
| title_short | Duck plague virus UL47 gene affects the release and cell-to-cell spread of the virus and its deletion strains can provide strong protection for ducks |
| title_sort | duck plague virus ul47 gene affects the release and cell to cell spread of the virus and its deletion strains can provide strong protection for ducks |
| topic | Duck plague virus UL47 Life cycle Virulence Immune protection |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125003311 |
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