Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products
Artemisa herba-alba is an important medicinal herb with immense pharmacological potential. The present study evaluated the effects of Artemisia herba-alba extract (AHE) on peripheral artery disease in diabetic rats via inhibition of advanced glycation end products (AGEs). The in vitro AGE inhibitin...
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Elsevier
2022-08-01
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| Series: | Kuwait Journal of Science |
| Online Access: | https://journalskuwait.org/kjs/index.php/KJS/article/view/18879 |
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| author | Li Sun Fang Liu Jianting Li Pei Sun |
| author_facet | Li Sun Fang Liu Jianting Li Pei Sun |
| author_sort | Li Sun |
| collection | DOAJ |
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Artemisa herba-alba is an important medicinal herb with immense pharmacological potential. The present study evaluated the effects of Artemisia herba-alba extract (AHE) on peripheral artery disease in diabetic rats via inhibition of advanced glycation end products (AGEs). The in vitro AGE inhibiting potential of AHE was determined by spectrofluorimetric method. The blood glucose levels and HbA1c (A1C) of the rats from each group were determined by automatic analyser. The levels of AGEs in vascular smooth muscle cells (VSMCs) of different rat groups were determined by western blotting. Expression of COX-1 and COX-2 were determined by qRT-PCR. Results showed that AHE inhibited the AGEs formation in vitro and exhibited an IC50 of 45 µg/mL which was significantly lower than that of standard AGEs inhibitor aminoguanidine (IC50: 60 µg/mL). Analysis of metabolic profiles revealed that AHE normalised the blood glucose, cholesterol, and triglycerides with no apparent changes on Hb1Ac levels. Western blot analysis showed that AHE exhibited protective effects in VSMCs of diabetic rats by inhibiting the production of AGEs. Moreover, the expression of COX-2 was inhibited by AHE in diabetic rats. However, the expression of COX-1 remained unaltered. Collectively, the results revealed AHE inhibits AGEs formation in vitro and in VSMCs of diabetic rats. These findings point towards the potential of AHE in the management of diabetic peripheral artery disease.
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| format | Article |
| id | doaj-art-e295b75db4c84e97a5752e6a8654fca7 |
| institution | Kabale University |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
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| series | Kuwait Journal of Science |
| spelling | doaj-art-e295b75db4c84e97a5752e6a8654fca72025-08-20T03:26:44ZengElsevierKuwait Journal of Science2307-41082307-41162022-08-01501A10.48129/kjs.18879Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end productsLi Sun 0Fang Liu 1Jianting Li 2Pei Sun3Department of Intensive Care Unit, Central Hospital Affiliated to Shandong First Medical University, No. 105, Jiefang Road, Jinan City, Shandong, 250013, China.Department of Kidney Disease Unit& Dialysis, Central Hospital Affiliated to Shandong First Medical University, No. 105, Jiefang Road, Jinan City, Shandong, 250013, China.Department of Health, Vertigo Examination Room, Central Hospital Affiliated to Shandong First Medical University, No. 105, Jiefang Road, Jinan City, Shandong, 250013, China.Department of Endocrinology, Central Hospital Affiliated to Shandong First Medical University, No. 105, Jiefang Road, Jinan City, Shandong, 250013, China. Artemisa herba-alba is an important medicinal herb with immense pharmacological potential. The present study evaluated the effects of Artemisia herba-alba extract (AHE) on peripheral artery disease in diabetic rats via inhibition of advanced glycation end products (AGEs). The in vitro AGE inhibiting potential of AHE was determined by spectrofluorimetric method. The blood glucose levels and HbA1c (A1C) of the rats from each group were determined by automatic analyser. The levels of AGEs in vascular smooth muscle cells (VSMCs) of different rat groups were determined by western blotting. Expression of COX-1 and COX-2 were determined by qRT-PCR. Results showed that AHE inhibited the AGEs formation in vitro and exhibited an IC50 of 45 µg/mL which was significantly lower than that of standard AGEs inhibitor aminoguanidine (IC50: 60 µg/mL). Analysis of metabolic profiles revealed that AHE normalised the blood glucose, cholesterol, and triglycerides with no apparent changes on Hb1Ac levels. Western blot analysis showed that AHE exhibited protective effects in VSMCs of diabetic rats by inhibiting the production of AGEs. Moreover, the expression of COX-2 was inhibited by AHE in diabetic rats. However, the expression of COX-1 remained unaltered. Collectively, the results revealed AHE inhibits AGEs formation in vitro and in VSMCs of diabetic rats. These findings point towards the potential of AHE in the management of diabetic peripheral artery disease. https://journalskuwait.org/kjs/index.php/KJS/article/view/18879 |
| spellingShingle | Li Sun Fang Liu Jianting Li Pei Sun Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products Kuwait Journal of Science |
| title | Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products |
| title_full | Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products |
| title_fullStr | Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products |
| title_full_unstemmed | Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products |
| title_short | Protective effects of Artemisia herba-alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products |
| title_sort | protective effects of artemisia herba alba in diabetic peripheral artery disease mediated via inhibition of advanced glycation end products |
| url | https://journalskuwait.org/kjs/index.php/KJS/article/view/18879 |
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