Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia

This study aimed to isolate and identify a fungal community on the carapace of the European pond turtle (Emys orbicularis) and to discuss their potential role as constituents of the turtle mycobiome. The study was conducted during the summer of 2020 within the special nature reserve Deliblato Sands...

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Main Authors: Stupar Miloš Č., Savković Željko D., Ljaljević-Grbić Milica V., Krizmanić Imre I.
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2025-01-01
Series:Archives of Biological Sciences
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Online Access:https://doiserbia.nb.rs/img/doi/0354-4664/2025/0354-46642500015S.pdf
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author Stupar Miloš Č.
Savković Željko D.
Ljaljević-Grbić Milica V.
Krizmanić Imre I.
author_facet Stupar Miloš Č.
Savković Željko D.
Ljaljević-Grbić Milica V.
Krizmanić Imre I.
author_sort Stupar Miloš Č.
collection DOAJ
description This study aimed to isolate and identify a fungal community on the carapace of the European pond turtle (Emys orbicularis) and to discuss their potential role as constituents of the turtle mycobiome. The study was conducted during the summer of 2020 within the special nature reserve Deliblato Sands (Deliblatska Peščara), situated in northern Serbia. We used a direct microscopy technique along with culture-based methods and molecular barcoding of the internal transcribed spacer (ITS) region. A plethora of fungal spores were documented as constituents of the biofilm present on the carapace surfaces. A total of 12 fungal isolates from the E. orbicularis carapace were identified, belonging to Alternaria, Aureobasidium, Cladosporium, Curvularia, Didymella, Microsphaeropsis, Pseudopithomyces, Naganishia, Rhodotorula, and Mucor genera. Ecological analyses showed that the majority of documented fungal species originated from terrestrial and aquatic environments, and many could be regarded as potential chelonian pathogens. The study presents pioneering research on fungal dwellers of E. orbicularis in Serbia.
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spelling doaj-art-e2949d70f1d342c184dcf6bc0ef0e45b2025-08-20T02:46:19ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392025-01-0177218519510.2298/ABS250410015S0354-46642500015SDiversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, SerbiaStupar Miloš Č.0https://orcid.org/0000-0002-9258-5688Savković Željko D.1https://orcid.org/0000-0002-6678-4958Ljaljević-Grbić Milica V.2https://orcid.org/0000-0003-0541-7713Krizmanić Imre I.3https://orcid.org/0000-0001-9684-8732Faculty of Biology, University of Belgrade, Belgrade, SerbiaFaculty of Biology, University of Belgrade, Belgrade, SerbiaFaculty of Biology, University of Belgrade, Belgrade, SerbiaFaculty of Biology, University of Belgrade, Belgrade, SerbiaThis study aimed to isolate and identify a fungal community on the carapace of the European pond turtle (Emys orbicularis) and to discuss their potential role as constituents of the turtle mycobiome. The study was conducted during the summer of 2020 within the special nature reserve Deliblato Sands (Deliblatska Peščara), situated in northern Serbia. We used a direct microscopy technique along with culture-based methods and molecular barcoding of the internal transcribed spacer (ITS) region. A plethora of fungal spores were documented as constituents of the biofilm present on the carapace surfaces. A total of 12 fungal isolates from the E. orbicularis carapace were identified, belonging to Alternaria, Aureobasidium, Cladosporium, Curvularia, Didymella, Microsphaeropsis, Pseudopithomyces, Naganishia, Rhodotorula, and Mucor genera. Ecological analyses showed that the majority of documented fungal species originated from terrestrial and aquatic environments, and many could be regarded as potential chelonian pathogens. The study presents pioneering research on fungal dwellers of E. orbicularis in Serbia.https://doiserbia.nb.rs/img/doi/0354-4664/2025/0354-46642500015S.pdfcheloniafungiturtle carapacepathogens
spellingShingle Stupar Miloš Č.
Savković Željko D.
Ljaljević-Grbić Milica V.
Krizmanić Imre I.
Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
Archives of Biological Sciences
chelonia
fungi
turtle carapace
pathogens
title Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
title_full Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
title_fullStr Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
title_full_unstemmed Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
title_short Diversity of fungi isolated from the carapace of the European pond turtle (Emys orbicularis, L. 1758) in South Banat, Serbia
title_sort diversity of fungi isolated from the carapace of the european pond turtle emys orbicularis l 1758 in south banat serbia
topic chelonia
fungi
turtle carapace
pathogens
url https://doiserbia.nb.rs/img/doi/0354-4664/2025/0354-46642500015S.pdf
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