Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells
Acid-sensing ion channels, a proton-gated cation channel, can be activated by low extracellular pH and involved in pathogenesis of some tumors such as glioma and breast cancer. However, the role of acid-sensing ion channels in the growth of lung cancer cell is unclear. In this study, we investigated...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-05-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317705750 |
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| _version_ | 1849396019403423744 |
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| author | Yu Wu Bo Gao Qiu-Ju Xiong Yu-Chan Wang Da-Ke Huang Wen-Ning Wu |
| author_facet | Yu Wu Bo Gao Qiu-Ju Xiong Yu-Chan Wang Da-Ke Huang Wen-Ning Wu |
| author_sort | Yu Wu |
| collection | DOAJ |
| description | Acid-sensing ion channels, a proton-gated cation channel, can be activated by low extracellular pH and involved in pathogenesis of some tumors such as glioma and breast cancer. However, the role of acid-sensing ion channels in the growth of lung cancer cell is unclear. In this study, we investigated the expression of acid-sensing ion channels in human lung cancer cell line A549 and their possible role in proliferation and migration of A549 cells. The results show that acid-sensing ion channel 1, acid-sensing ion channel 2, and acid-sensing ion channel 3 are expressed in A549 cells at the messenger RNA and protein levels, and acid-sensing ion channel–like currents were elicited by extracellular acid stimuli. Moreover, we found that acidic extracellular medium or overexpressing acid-sensing ion channel 1a promotes proliferation and migration of A549 cells. In addition psalmotoxin 1, a specific acid-sensing ion channel 1a inhibitor, or acid-sensing ion channel 1a knockdown can abolish the effect of acid stimuli on A549 cells. In addition, acid-sensing ion channels mediate increase of [Ca 2+ ] i induced by low extracellular pH in A549 cells. All these results indicate that acid-sensing ion channel–calcium signal mediate lung cancer cell proliferation and migration induced by extracellular acidosis, and acid-sensing ion channels may serve as a prognostic marker and a therapeutic target for lung cancer. |
| format | Article |
| id | doaj-art-e293302308e849e2b711ee906ad9948e |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-e293302308e849e2b711ee906ad9948e2025-08-20T03:39:27ZengSAGE PublishingTumor Biology1423-03802017-05-013910.1177/1010428317705750Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cellsYu Wu0Bo Gao1Qiu-Ju Xiong2Yu-Chan Wang3Da-Ke Huang4Wen-Ning Wu5Department of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, P.R. ChinaDepartment of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, P.R. ChinaDepartment of Pain Management, Wuhan Pu’ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. ChinaDepartment of Pharmacology, School of Basic Medical Sciences, Anhui Medical University, Hefei, P.R. ChinaSynthetic Laboratory, School of Basic Medical Sciences, Anhui Medical University, Hefei, P.R. ChinaKey Laboratory of Anti-inflammatory and Immunopharmacology, Anhui Medical University, Hefei, P.R. ChinaAcid-sensing ion channels, a proton-gated cation channel, can be activated by low extracellular pH and involved in pathogenesis of some tumors such as glioma and breast cancer. However, the role of acid-sensing ion channels in the growth of lung cancer cell is unclear. In this study, we investigated the expression of acid-sensing ion channels in human lung cancer cell line A549 and their possible role in proliferation and migration of A549 cells. The results show that acid-sensing ion channel 1, acid-sensing ion channel 2, and acid-sensing ion channel 3 are expressed in A549 cells at the messenger RNA and protein levels, and acid-sensing ion channel–like currents were elicited by extracellular acid stimuli. Moreover, we found that acidic extracellular medium or overexpressing acid-sensing ion channel 1a promotes proliferation and migration of A549 cells. In addition psalmotoxin 1, a specific acid-sensing ion channel 1a inhibitor, or acid-sensing ion channel 1a knockdown can abolish the effect of acid stimuli on A549 cells. In addition, acid-sensing ion channels mediate increase of [Ca 2+ ] i induced by low extracellular pH in A549 cells. All these results indicate that acid-sensing ion channel–calcium signal mediate lung cancer cell proliferation and migration induced by extracellular acidosis, and acid-sensing ion channels may serve as a prognostic marker and a therapeutic target for lung cancer.https://doi.org/10.1177/1010428317705750 |
| spellingShingle | Yu Wu Bo Gao Qiu-Ju Xiong Yu-Chan Wang Da-Ke Huang Wen-Ning Wu Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells Tumor Biology |
| title | Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells |
| title_full | Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells |
| title_fullStr | Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells |
| title_full_unstemmed | Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells |
| title_short | Acid-sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of A549 cells |
| title_sort | acid sensing ion channels contribute to the effect of extracellular acidosis on proliferation and migration of a549 cells |
| url | https://doi.org/10.1177/1010428317705750 |
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