Exploring the Potential and Advancements of Circular RNA Therapeutics
ABSTRACT Messenger RNA (mRNA) technology is revolutionizing the pharmaceutical industry owing to its superior safety profile, manufacturing capabilities, and potential applications in previously undruggable therapeutic targets. In addition to linear mRNA, such as conventional mRNA, self‐amplifying m...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-08-01
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| Series: | Exploration |
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| Online Access: | https://doi.org/10.1002/EXP.20240044 |
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| author | Lei Wang Lianqing Wang Chunbo Dong Jialong Liu Guoxin Cui Shan Gao Zhida Liu |
| author_facet | Lei Wang Lianqing Wang Chunbo Dong Jialong Liu Guoxin Cui Shan Gao Zhida Liu |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | ABSTRACT Messenger RNA (mRNA) technology is revolutionizing the pharmaceutical industry owing to its superior safety profile, manufacturing capabilities, and potential applications in previously undruggable therapeutic targets. In addition to linear mRNA, such as conventional mRNA, self‐amplifying mRNA, and trans‐amplifying mRNA, circular mRNA has emerged as a promising candidate. Circular RNA (circRNA) is a class of single‐stranded RNA with a covalently closed loop structure that offers enhanced stability compared to linear RNA by resisting degradation from RNases. Recent studies have revolutionized our understanding of their biological functions, surpassing the notion that they are merely byproducts of aberrant splicing events. Given the remarkable success achieved in cancer and SARS‐CoV‐2/monkeypox virus (MPXV) vaccines, circRNA is being intensively investigated for gene and cell therapies. In this review, we provide an overview of circRNA biogenesis mechanisms in vivo, along with synthesis strategies in vitro, while discussing translation regulation mechanisms and quality control processes involved in circRNA production. Furthermore, we explore the potential application scenarios for circRNAs. |
| format | Article |
| id | doaj-art-e28ddbe6296d4197a5e8524961182311 |
| institution | Kabale University |
| issn | 2766-8509 2766-2098 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Exploration |
| spelling | doaj-art-e28ddbe6296d4197a5e85249611823112025-08-26T10:33:05ZengWileyExploration2766-85092766-20982025-08-0154n/an/a10.1002/EXP.20240044Exploring the Potential and Advancements of Circular RNA TherapeuticsLei Wang0Lianqing Wang1Chunbo Dong2Jialong Liu3Guoxin Cui4Shan Gao5Zhida Liu6Shanxi Academy of Advanced Research and Innovation Taiyuan ChinaCenter of Translational Medicine Zibo Central Hospital Zibo ChinaShanxi Academy of Advanced Research and Innovation Taiyuan ChinaMOE Key Laboratory of Coal Environmental Pathogenicity and Prevention Shanxi Medical University Taiyuan ChinaRed Sea Research Center Biological and Environmental Science and Engineering Division King Abdullah University of Science and Technology (KAUST) Thuwal Saudi ArabiaShanxi Academy of Advanced Research and Innovation Taiyuan ChinaShanxi Academy of Advanced Research and Innovation Taiyuan ChinaABSTRACT Messenger RNA (mRNA) technology is revolutionizing the pharmaceutical industry owing to its superior safety profile, manufacturing capabilities, and potential applications in previously undruggable therapeutic targets. In addition to linear mRNA, such as conventional mRNA, self‐amplifying mRNA, and trans‐amplifying mRNA, circular mRNA has emerged as a promising candidate. Circular RNA (circRNA) is a class of single‐stranded RNA with a covalently closed loop structure that offers enhanced stability compared to linear RNA by resisting degradation from RNases. Recent studies have revolutionized our understanding of their biological functions, surpassing the notion that they are merely byproducts of aberrant splicing events. Given the remarkable success achieved in cancer and SARS‐CoV‐2/monkeypox virus (MPXV) vaccines, circRNA is being intensively investigated for gene and cell therapies. In this review, we provide an overview of circRNA biogenesis mechanisms in vivo, along with synthesis strategies in vitro, while discussing translation regulation mechanisms and quality control processes involved in circRNA production. Furthermore, we explore the potential application scenarios for circRNAs.https://doi.org/10.1002/EXP.20240044back‐splicingcircRNAIRESmRNAribozymetherapeutics |
| spellingShingle | Lei Wang Lianqing Wang Chunbo Dong Jialong Liu Guoxin Cui Shan Gao Zhida Liu Exploring the Potential and Advancements of Circular RNA Therapeutics Exploration back‐splicing circRNA IRES mRNA ribozyme therapeutics |
| title | Exploring the Potential and Advancements of Circular RNA Therapeutics |
| title_full | Exploring the Potential and Advancements of Circular RNA Therapeutics |
| title_fullStr | Exploring the Potential and Advancements of Circular RNA Therapeutics |
| title_full_unstemmed | Exploring the Potential and Advancements of Circular RNA Therapeutics |
| title_short | Exploring the Potential and Advancements of Circular RNA Therapeutics |
| title_sort | exploring the potential and advancements of circular rna therapeutics |
| topic | back‐splicing circRNA IRES mRNA ribozyme therapeutics |
| url | https://doi.org/10.1002/EXP.20240044 |
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