Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid
Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an induc...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2012-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912601695 |
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| author | You-ping XU Hui-ying CHEN Xin ZHOU Xin-zhong CAI |
| author_facet | You-ping XU Hui-ying CHEN Xin ZHOU Xin-zhong CAI |
| author_sort | You-ping XU |
| collection | DOAJ |
| description | Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an inducer of plant hypersensitive response (HR) and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNM1 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNNI1 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfHNNI1 induced plant HR in a dose-dependent manner. CfHNNI1-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and def1 tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways. |
| format | Article |
| id | doaj-art-e28c772e950a4f3aa80f2d980ed724c8 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2012-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e28c772e950a4f3aa80f2d980ed724c82025-08-20T03:57:59ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192012-10-0111101665167410.1016/S2095-3119(12)60169-5Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic AcidYou-ping XU0Hui-ying CHEN1Xin ZHOU2Xin-zhong CAI3Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China; Center of Analysis and Measurement, Zhejiang University, Hangzhou 310058, P.R. China; XU You-pingInstitute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. ChinaInstitute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. ChinaInstitute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China; Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture/Zhejiang University, Hangzhou 310058, P.R. China; Correspondence CAI Xin-zhong, Tel: +86-571-88982936, Fax: +86-571-88982936Nonhost resistance is a phenomenon that enables plants to protect themselves against the majority of potential pathogens, and thus has a great potential for application in plant protection. We recently found that CfHNNI1 (for Cladosporium fulvum host and nonhost plant necrosis inducer 1) is an inducer of plant hypersensitive response (HR) and nonhost resistance. In this study, its functional mechanism was analyzed. CfHNM1 was a single copy gene in C. fulvum genome. The functional ORF of the CfHNNI1 cDNA was ATG3-TAG780, which showed homology with genes encoding bZIP transcription factors. The functional ORF included in frame an inner one ATG273-TAG780, which was sufficient to induce HR in plants. CfHNNI1 induced plant HR in a dose-dependent manner. CfHNNI1-induced necrosis in NahG transgenic tomato plants was significantly stronger than that in their wild type controls. However, the necrosis in Nr and def1 tomato mutants was similar to that in their corresponding wild type plants. These data demonstrate that induction of HR and nonhost resistance by CfHNNI1 is negatively regulated by salicylic acid signalling pathway but independent of ethylene and jasmonic acid signalling pathways.http://www.sciencedirect.com/science/article/pii/S2095311912601695CfHNNI1Cladosporium fulvumethylenehypersensitive responsejasmonic acidnonhost resistance |
| spellingShingle | You-ping XU Hui-ying CHEN Xin ZHOU Xin-zhong CAI Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid Journal of Integrative Agriculture CfHNNI1 Cladosporium fulvum ethylene hypersensitive response jasmonic acid nonhost resistance |
| title | Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid |
| title_full | Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid |
| title_fullStr | Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid |
| title_full_unstemmed | Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid |
| title_short | Induction of Hypersensitive Response and Nonhost Resistance by a Cladosporium fulvum Elicitor CfHNNI1 is Dose-Dependent and Negatively Regulated by Salicylic Acid |
| title_sort | induction of hypersensitive response and nonhost resistance by a cladosporium fulvum elicitor cfhnni1 is dose dependent and negatively regulated by salicylic acid |
| topic | CfHNNI1 Cladosporium fulvum ethylene hypersensitive response jasmonic acid nonhost resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912601695 |
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