Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice
In this work, the authors compared microCT and in vivo ultrasonography in terms of accuracy and efficacy for measuring the volume of various organs in mice. Two quantification protocols were applied: ellipsoidal volume measuring maximum diameters in all three axes in both imaging systems and manual...
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MDPI AG
2025-06-01
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| Series: | Journal of Imaging |
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| Online Access: | https://www.mdpi.com/2313-433X/11/6/200 |
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| author | Juan Jose Jimenez Catalan Marina Ferrer Clotas Juan Antonio Camara Serrano |
| author_facet | Juan Jose Jimenez Catalan Marina Ferrer Clotas Juan Antonio Camara Serrano |
| author_sort | Juan Jose Jimenez Catalan |
| collection | DOAJ |
| description | In this work, the authors compared microCT and in vivo ultrasonography in terms of accuracy and efficacy for measuring the volume of various organs in mice. Two quantification protocols were applied: ellipsoidal volume measuring maximum diameters in all three axes in both imaging systems and manual delineation of organ borders in microCT studies. The results were compared with ex vivo volumes. In general, both imaging techniques and quantification protocols are accurate, but ultrasound is faster in both acquisition and analysis. The only accurate method for heart volume measurement is manual segmentation on microCT. For the ovary, none of the techniques and protocols had a positive correlation with ex vivo volume. The three-diameter method can be used for ellipsoid organs because of its rapidity, but for more irregular structures, manual segmentation is recommended, although it is time-consuming. |
| format | Article |
| id | doaj-art-e2888cc1c68b42fead65469305de38e6 |
| institution | OA Journals |
| issn | 2313-433X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Imaging |
| spelling | doaj-art-e2888cc1c68b42fead65469305de38e62025-08-20T02:20:58ZengMDPI AGJournal of Imaging2313-433X2025-06-0111620010.3390/jimaging11060200Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in MiceJuan Jose Jimenez Catalan0Marina Ferrer Clotas1Juan Antonio Camara Serrano2Preclinical Imaging Platform, Vall d’Hebron Institute of Oncology, 08035 Barcelona, SpainGnotobiotic Core and Mouse Metabolism Core, University of California San Francisco, San Francisco, CA 94143, USAPreclinical Therapeutics Core, University of California San Francisco, San Francisco, CA 94158, USAIn this work, the authors compared microCT and in vivo ultrasonography in terms of accuracy and efficacy for measuring the volume of various organs in mice. Two quantification protocols were applied: ellipsoidal volume measuring maximum diameters in all three axes in both imaging systems and manual delineation of organ borders in microCT studies. The results were compared with ex vivo volumes. In general, both imaging techniques and quantification protocols are accurate, but ultrasound is faster in both acquisition and analysis. The only accurate method for heart volume measurement is manual segmentation on microCT. For the ovary, none of the techniques and protocols had a positive correlation with ex vivo volume. The three-diameter method can be used for ellipsoid organs because of its rapidity, but for more irregular structures, manual segmentation is recommended, although it is time-consuming.https://www.mdpi.com/2313-433X/11/6/200microCTultrasoundpreclinical imagingorgan volumemice |
| spellingShingle | Juan Jose Jimenez Catalan Marina Ferrer Clotas Juan Antonio Camara Serrano Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice Journal of Imaging microCT ultrasound preclinical imaging organ volume mice |
| title | Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice |
| title_full | Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice |
| title_fullStr | Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice |
| title_full_unstemmed | Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice |
| title_short | Comparative Analysis of Ultrasonography and MicroCT Imaging for Organ Size Evaluation in Mice |
| title_sort | comparative analysis of ultrasonography and microct imaging for organ size evaluation in mice |
| topic | microCT ultrasound preclinical imaging organ volume mice |
| url | https://www.mdpi.com/2313-433X/11/6/200 |
| work_keys_str_mv | AT juanjosejimenezcatalan comparativeanalysisofultrasonographyandmicroctimagingfororgansizeevaluationinmice AT marinaferrerclotas comparativeanalysisofultrasonographyandmicroctimagingfororgansizeevaluationinmice AT juanantoniocamaraserrano comparativeanalysisofultrasonographyandmicroctimagingfororgansizeevaluationinmice |