Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains
The research focuses on the study of phagocyte activity of blood neutrophilic granulocytes, monocytes, and eosinophils under the exposure to methicillin-resistant strains of Staphylococcus aureus bacteria. The subjects of the research were represented by blood neutrophilic granulocytes, monocytes, a...
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2022-04-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/883 |
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| author | O. A. Kolenchukova V. D. Belenuk |
| author_facet | O. A. Kolenchukova V. D. Belenuk |
| author_sort | O. A. Kolenchukova |
| collection | DOAJ |
| description | The research focuses on the study of phagocyte activity of blood neutrophilic granulocytes, monocytes, and eosinophils under the exposure to methicillin-resistant strains of Staphylococcus aureus bacteria. The subjects of the research were represented by blood neutrophilic granulocytes, monocytes, and eosinophils, isolated from healthy people, as well as methicillin-resistant and methicillin-sensitive S.aureus bacteria strains (MRSA and MSSA). Phagocytosis functions (phagocytic number and phagocytic index) were estimated by FITC-labeled bacteria. The analysis of stained cells was performed using FC-500 flow cytofluorimeter (Beckman Coulter, USA) for whole peripheral blood. Phagocytosis research resulted in the following findings. In response to MRSA, the percentage of neutrophils involved in phagocytosis and average number of bacteria being present inside the cells increase as compared to those within sensitive strains. In response to MRSA exposure, the phagocytic index is higher in monocytes with CD14+CD16¯phenotype. At the same time, phagocytic number is higher in non-classic populations of monocytes CD14+CD16+and CD14lowCD16+. Moreover, it should be noted that the classical type of monocytes CD14+CD16¯tends to be activated faster in regard to MRSA, but phagocytosis efficiency is lowered. Blood eosinophils also actively respond to MRSA. Thus, changes in functional activity of neutrophilic granulocytes, monocytes, and eosinophils in peripheral blood were established during MRSA induction. The bacterial receptor apparatus changes due to the modification of cellular wall as a result of acquired resistance to antibiotics. |
| format | Article |
| id | doaj-art-e2884925bf034b4a98a4f3f6ae5b4fa0 |
| institution | Kabale University |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2022-04-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-e2884925bf034b4a98a4f3f6ae5b4fa02025-08-20T03:59:08ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902022-04-01671-24810.37489/0235-2990-2022-67-1-2-4-8821Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> StrainsO. A. Kolenchukova0V. D. Belenuk1Institute for Fundamental Biology and Biotechnologies of Siberian Federal University of the Ministry of Education and Science of the Russian Federation; Federal Research Center «Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences»Federal Research Center «Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences»The research focuses on the study of phagocyte activity of blood neutrophilic granulocytes, monocytes, and eosinophils under the exposure to methicillin-resistant strains of Staphylococcus aureus bacteria. The subjects of the research were represented by blood neutrophilic granulocytes, monocytes, and eosinophils, isolated from healthy people, as well as methicillin-resistant and methicillin-sensitive S.aureus bacteria strains (MRSA and MSSA). Phagocytosis functions (phagocytic number and phagocytic index) were estimated by FITC-labeled bacteria. The analysis of stained cells was performed using FC-500 flow cytofluorimeter (Beckman Coulter, USA) for whole peripheral blood. Phagocytosis research resulted in the following findings. In response to MRSA, the percentage of neutrophils involved in phagocytosis and average number of bacteria being present inside the cells increase as compared to those within sensitive strains. In response to MRSA exposure, the phagocytic index is higher in monocytes with CD14+CD16¯phenotype. At the same time, phagocytic number is higher in non-classic populations of monocytes CD14+CD16+and CD14lowCD16+. Moreover, it should be noted that the classical type of monocytes CD14+CD16¯tends to be activated faster in regard to MRSA, but phagocytosis efficiency is lowered. Blood eosinophils also actively respond to MRSA. Thus, changes in functional activity of neutrophilic granulocytes, monocytes, and eosinophils in peripheral blood were established during MRSA induction. The bacterial receptor apparatus changes due to the modification of cellular wall as a result of acquired resistance to antibiotics.https://www.antibiotics-chemotherapy.ru/jour/article/view/883neutrophilic granulocytesmonocyteseosinophilsphagocytosisstaphylococcus aureusmethicillin resistance |
| spellingShingle | O. A. Kolenchukova V. D. Belenuk Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains Антибиотики и Химиотерапия neutrophilic granulocytes monocytes eosinophils phagocytosis staphylococcus aureus methicillin resistance |
| title | Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains |
| title_full | Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains |
| title_fullStr | Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains |
| title_full_unstemmed | Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains |
| title_short | Phagocyte Activity in Response to Exposure to Methicillin-Resistant <i>Staphylococcus aureus</i> Strains |
| title_sort | phagocyte activity in response to exposure to methicillin resistant i staphylococcus aureus i strains |
| topic | neutrophilic granulocytes monocytes eosinophils phagocytosis staphylococcus aureus methicillin resistance |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/883 |
| work_keys_str_mv | AT oakolenchukova phagocyteactivityinresponsetoexposuretomethicillinresistantistaphylococcusaureusistrains AT vdbelenuk phagocyteactivityinresponsetoexposuretomethicillinresistantistaphylococcusaureusistrains |