“One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent
Caffeic acid phenethyl ester (CAPE) represents a valuable ester of caffeic acid, which, over time, has demonstrated remarkable pharmacological properties. In general, the ester is obtained in organic solvents, especially by the esterification reaction of caffeic acid (CA) and 2-phenylethanol (PE). I...
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MDPI AG
2025-01-01
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| Series: | Biomolecules |
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| author | Maria Roberta Tripon Camelia Tulcan Simona Marc Dorin-Dumitru Camen Cristina Paul |
| author_facet | Maria Roberta Tripon Camelia Tulcan Simona Marc Dorin-Dumitru Camen Cristina Paul |
| author_sort | Maria Roberta Tripon |
| collection | DOAJ |
| description | Caffeic acid phenethyl ester (CAPE) represents a valuable ester of caffeic acid, which, over time, has demonstrated remarkable pharmacological properties. In general, the ester is obtained in organic solvents, especially by the esterification reaction of caffeic acid (CA) and 2-phenylethanol (PE). In this context, the purpose of this study was the use of the “one pot” system to synthesize CAPE through biocatalysis with various lipases in a choline-chloride-based DES system, employing the “2-in-1” concept, where one of the substrates functions as both reactant and solvent. The synthesis process of CAPE is contingent on the molar ratio between CA and PE; thus, this factor was the primary subject of investigation, with different molar ratios of CA and PE being studied. Furthermore, the impact of temperature, time, the nature of the biocatalyst, and the water loading of the DES system was also examined. This ‘green’ synthesis method, which has demonstrated encouraging reaction yields (%), could secure and maintain the therapeutic potential of CAPE, mainly due to the non-toxic character of the reaction medium. |
| format | Article |
| id | doaj-art-e284e4b27f584711b6ac73fff18716a1 |
| institution | DOAJ |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-e284e4b27f584711b6ac73fff18716a12025-08-20T02:44:59ZengMDPI AGBiomolecules2218-273X2025-01-0115218110.3390/biom15020181“One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic SolventMaria Roberta Tripon0Camelia Tulcan1Simona Marc2Dorin-Dumitru Camen3Cristina Paul4Faculty of Engineering and Applied Technologies, University of Life Sciences “King Mihai I” from Timișoara, Calea Aradului No. 119, 300645 Timișoara, RomaniaFaculty of Engineering and Applied Technologies, University of Life Sciences “King Mihai I” from Timișoara, Calea Aradului No. 119, 300645 Timișoara, RomaniaFaculty of Veterinary Medicine, University of Life Sciences “King Mihai I” from Timișoara, Calea Aradului No. 119, 300645 Timișoara, RomaniaFaculty of Engineering and Applied Technologies, University of Life Sciences “King Mihai I” from Timișoara, Calea Aradului No. 119, 300645 Timișoara, RomaniaDepartment of Applied Chemistry and Engineering of Organic and Natural Compounds, Faculty of Chemical Engineering, Biotechnologies and Environmental Protection, Politehnica University Timisoara, Vasile Pârvan No. 6, 300223 Timișoara, RomaniaCaffeic acid phenethyl ester (CAPE) represents a valuable ester of caffeic acid, which, over time, has demonstrated remarkable pharmacological properties. In general, the ester is obtained in organic solvents, especially by the esterification reaction of caffeic acid (CA) and 2-phenylethanol (PE). In this context, the purpose of this study was the use of the “one pot” system to synthesize CAPE through biocatalysis with various lipases in a choline-chloride-based DES system, employing the “2-in-1” concept, where one of the substrates functions as both reactant and solvent. The synthesis process of CAPE is contingent on the molar ratio between CA and PE; thus, this factor was the primary subject of investigation, with different molar ratios of CA and PE being studied. Furthermore, the impact of temperature, time, the nature of the biocatalyst, and the water loading of the DES system was also examined. This ‘green’ synthesis method, which has demonstrated encouraging reaction yields (%), could secure and maintain the therapeutic potential of CAPE, mainly due to the non-toxic character of the reaction medium.https://www.mdpi.com/2218-273X/15/2/181caffeic acid phenethyl ester (CAPE)enzymatic synthesisdeep eutectic solventreaction optimization |
| spellingShingle | Maria Roberta Tripon Camelia Tulcan Simona Marc Dorin-Dumitru Camen Cristina Paul “One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent Biomolecules caffeic acid phenethyl ester (CAPE) enzymatic synthesis deep eutectic solvent reaction optimization |
| title | “One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent |
| title_full | “One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent |
| title_fullStr | “One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent |
| title_full_unstemmed | “One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent |
| title_short | “One Pot” Enzymatic Synthesis of Caffeic Acid Phenethyl Ester in Deep Eutectic Solvent |
| title_sort | one pot enzymatic synthesis of caffeic acid phenethyl ester in deep eutectic solvent |
| topic | caffeic acid phenethyl ester (CAPE) enzymatic synthesis deep eutectic solvent reaction optimization |
| url | https://www.mdpi.com/2218-273X/15/2/181 |
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