Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques

Odor-induced sweetness enhancement (OISE) is an effective approach to lower sugar intake. In this study, static and dynamic sensory evaluations, combined with molecular docking, were used to explore the mechanism underlying sweetness enhancement in fructose solutions induced by watermelon juice odor...

Full description

Saved in:
Bibliographic Details
Main Authors: Yixin Dai, Shuang Sun, Fan Yang, Shaobo Zhen, Xiaoying Xiong, Ye Liu, Shuang Bi
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/14/6/1034
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850204474278150144
author Yixin Dai
Shuang Sun
Fan Yang
Shaobo Zhen
Xiaoying Xiong
Ye Liu
Shuang Bi
author_facet Yixin Dai
Shuang Sun
Fan Yang
Shaobo Zhen
Xiaoying Xiong
Ye Liu
Shuang Bi
author_sort Yixin Dai
collection DOAJ
description Odor-induced sweetness enhancement (OISE) is an effective approach to lower sugar intake. In this study, static and dynamic sensory evaluations, combined with molecular docking, were used to explore the mechanism underlying sweetness enhancement in fructose solutions induced by watermelon juice odor compounds. Sensory evaluation results showed that the seven volatile odor compounds (VOCs) (ethyl acetate, ethyl propionate, octanal, (E,E)-2,4-hexadienal, (E)-2-octenal, methyl heptenone, and geranyl acetone) from watermelon juice could significantly increase the sweetness intensity of a 2.5% fructose solution, and the potency of OISE was significantly enhanced within 10 s. (E,E)-2,4-hexadienal, ethyl propionate, and methylheptenone showed the most significant OISE effects, which aligned with the sensory preference results. Further, molecular docking was used to explore the interactions between VOCs, fructose, and sweet receptors. The results showed that T1R2 was the main receptor for binding fructose and VOCs, and the interaction forces were primarily hydrogen bonds and hydrophobic interactions. In the presence of VOCs, the amino acid residues that formed hydrogen bonds with fructose were highly repetitive, with the main difference being the bond length, indicating the important role of flavor–sweetener receptor interactions in lowering fructose content and enhancing sweetness perception. Thus, this study provides a scientific basis for developing sugar-reduction technology based on aroma, in addition to enhancing sweetness.
format Article
id doaj-art-e27ecab4344a449f994426c7fd1bb395
institution OA Journals
issn 2304-8158
language English
publishDate 2025-03-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj-art-e27ecab4344a449f994426c7fd1bb3952025-08-20T02:11:17ZengMDPI AGFoods2304-81582025-03-01146103410.3390/foods14061034Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking TechniquesYixin Dai0Shuang Sun1Fan Yang2Shaobo Zhen3Xiaoying Xiong4Ye Liu5Shuang Bi6Beijing Engineering and Technology Research Center of Food Additives, Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University (BTBU), No. 11, Fucheng Road, Haidian District, Beijing 100048, ChinaBeijing Engineering and Technology Research Center of Food Additives, Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University (BTBU), No. 11, Fucheng Road, Haidian District, Beijing 100048, ChinaBeijing Engineering and Technology Research Center of Food Additives, Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University (BTBU), No. 11, Fucheng Road, Haidian District, Beijing 100048, ChinaSchool of Hotel Management, China University of Labor Relations, Beijing 100089, ChinaBeijing Engineering and Technology Research Center of Food Additives, Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University (BTBU), No. 11, Fucheng Road, Haidian District, Beijing 100048, ChinaBeijing Engineering and Technology Research Center of Food Additives, Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University (BTBU), No. 11, Fucheng Road, Haidian District, Beijing 100048, ChinaBeijing Engineering and Technology Research Center of Food Additives, Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Health, Beijing Technology and Business University (BTBU), No. 11, Fucheng Road, Haidian District, Beijing 100048, ChinaOdor-induced sweetness enhancement (OISE) is an effective approach to lower sugar intake. In this study, static and dynamic sensory evaluations, combined with molecular docking, were used to explore the mechanism underlying sweetness enhancement in fructose solutions induced by watermelon juice odor compounds. Sensory evaluation results showed that the seven volatile odor compounds (VOCs) (ethyl acetate, ethyl propionate, octanal, (E,E)-2,4-hexadienal, (E)-2-octenal, methyl heptenone, and geranyl acetone) from watermelon juice could significantly increase the sweetness intensity of a 2.5% fructose solution, and the potency of OISE was significantly enhanced within 10 s. (E,E)-2,4-hexadienal, ethyl propionate, and methylheptenone showed the most significant OISE effects, which aligned with the sensory preference results. Further, molecular docking was used to explore the interactions between VOCs, fructose, and sweet receptors. The results showed that T1R2 was the main receptor for binding fructose and VOCs, and the interaction forces were primarily hydrogen bonds and hydrophobic interactions. In the presence of VOCs, the amino acid residues that formed hydrogen bonds with fructose were highly repetitive, with the main difference being the bond length, indicating the important role of flavor–sweetener receptor interactions in lowering fructose content and enhancing sweetness perception. Thus, this study provides a scientific basis for developing sugar-reduction technology based on aroma, in addition to enhancing sweetness.https://www.mdpi.com/2304-8158/14/6/1034odor-induced sweetness enhancementwatermelon juicevolatile odor compoundssensory evaluationmolecular docking
spellingShingle Yixin Dai
Shuang Sun
Fan Yang
Shaobo Zhen
Xiaoying Xiong
Ye Liu
Shuang Bi
Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques
Foods
odor-induced sweetness enhancement
watermelon juice
volatile odor compounds
sensory evaluation
molecular docking
title Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques
title_full Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques
title_fullStr Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques
title_full_unstemmed Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques
title_short Revealing Volatile Odor Compounds in Watermelon Juice to Enhance Fructose Sweetness Perception: Sensory Evaluation and Molecular Docking Techniques
title_sort revealing volatile odor compounds in watermelon juice to enhance fructose sweetness perception sensory evaluation and molecular docking techniques
topic odor-induced sweetness enhancement
watermelon juice
volatile odor compounds
sensory evaluation
molecular docking
url https://www.mdpi.com/2304-8158/14/6/1034
work_keys_str_mv AT yixindai revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques
AT shuangsun revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques
AT fanyang revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques
AT shaobozhen revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques
AT xiaoyingxiong revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques
AT yeliu revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques
AT shuangbi revealingvolatileodorcompoundsinwatermelonjuicetoenhancefructosesweetnessperceptionsensoryevaluationandmoleculardockingtechniques