Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm
Semen cryopreservation is a crucial technology in the artificial insemination of livestock and poultry. It not only contributes to the conservation of germplasm resources but also facilitates the cross-regional exchange of high-quality breeding stock. In this study, 165 Duroc boars were selected for...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/10/1474 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850127496710717440 |
|---|---|
| author | Jiajun Zhang Meicheng Li Guangxiang Chen Chenyu Tao Bushe Li Hejun Zhang Hongyang Wang Wenjun Wang |
| author_facet | Jiajun Zhang Meicheng Li Guangxiang Chen Chenyu Tao Bushe Li Hejun Zhang Hongyang Wang Wenjun Wang |
| author_sort | Jiajun Zhang |
| collection | DOAJ |
| description | Semen cryopreservation is a crucial technology in the artificial insemination of livestock and poultry. It not only contributes to the conservation of germplasm resources but also facilitates the cross-regional exchange of high-quality breeding stock. In this study, 165 Duroc boars were selected for genome-wide genotyping, and the sperm freezing/thawing motility ratio (sperm recovery rate) was used as phenotypic data for genome-wide association analysis (GWAS). Considerable individual variations in sperm recovery rates (SRRs) were detected, and the sperm structure after cyropreservation was significantly better in highly freeze-tolerant individuals compared to non-freeze-tolerant ones. The heritability of the SRR was calculated and found to be 0.199 ± 0.158, representing low heritability. Through GWAS, eight single-nucleotide polymorphism (SNP) loci and four candidate genes (<i>SLC10A6</i>, <i>MYRF</i>, <i>GGA1</i>, and <i>UTRN</i>) were identified as being significantly associated with sperm freezing tolerance. Moreover, the dominant genotypes of four SNPs were finally determined to be valuable for identifying individuals with high sperm freezing tolerance. This study reveals the heritability of the sperm recovery rate and identifies molecular markers associated with sperm freezing tolerance in Duroc boars, which is of great significance for accelerating boar genetic improvement and enhancing the economic efficiency of pig breeding industry. |
| format | Article |
| id | doaj-art-e27d875bcbb54a0c883618b4fd2a2065 |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-e27d875bcbb54a0c883618b4fd2a20652025-08-20T02:33:39ZengMDPI AGAnimals2076-26152025-05-011510147410.3390/ani15101474Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar SpermJiajun Zhang0Meicheng Li1Guangxiang Chen2Chenyu Tao3Bushe Li4Hejun Zhang5Hongyang Wang6Wenjun Wang7College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaExperimental Training Center, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaShanghai Engineering Research Center of Breeding Pig, Shanghai 201106, ChinaShanghai Engineering Research Center of Breeding Pig, Shanghai 201106, ChinaKey Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry &Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaSemen cryopreservation is a crucial technology in the artificial insemination of livestock and poultry. It not only contributes to the conservation of germplasm resources but also facilitates the cross-regional exchange of high-quality breeding stock. In this study, 165 Duroc boars were selected for genome-wide genotyping, and the sperm freezing/thawing motility ratio (sperm recovery rate) was used as phenotypic data for genome-wide association analysis (GWAS). Considerable individual variations in sperm recovery rates (SRRs) were detected, and the sperm structure after cyropreservation was significantly better in highly freeze-tolerant individuals compared to non-freeze-tolerant ones. The heritability of the SRR was calculated and found to be 0.199 ± 0.158, representing low heritability. Through GWAS, eight single-nucleotide polymorphism (SNP) loci and four candidate genes (<i>SLC10A6</i>, <i>MYRF</i>, <i>GGA1</i>, and <i>UTRN</i>) were identified as being significantly associated with sperm freezing tolerance. Moreover, the dominant genotypes of four SNPs were finally determined to be valuable for identifying individuals with high sperm freezing tolerance. This study reveals the heritability of the sperm recovery rate and identifies molecular markers associated with sperm freezing tolerance in Duroc boars, which is of great significance for accelerating boar genetic improvement and enhancing the economic efficiency of pig breeding industry.https://www.mdpi.com/2076-2615/15/10/1474DurocGWASboar sperm freezabilitybiomarkercandidate gene |
| spellingShingle | Jiajun Zhang Meicheng Li Guangxiang Chen Chenyu Tao Bushe Li Hejun Zhang Hongyang Wang Wenjun Wang Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm Animals Duroc GWAS boar sperm freezability biomarker candidate gene |
| title | Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm |
| title_full | Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm |
| title_fullStr | Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm |
| title_full_unstemmed | Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm |
| title_short | Genome-Wide Association Study That Identifies Molecular Markers with Freezing Resistance in Duroc Boar Sperm |
| title_sort | genome wide association study that identifies molecular markers with freezing resistance in duroc boar sperm |
| topic | Duroc GWAS boar sperm freezability biomarker candidate gene |
| url | https://www.mdpi.com/2076-2615/15/10/1474 |
| work_keys_str_mv | AT jiajunzhang genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT meichengli genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT guangxiangchen genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT chenyutao genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT busheli genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT hejunzhang genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT hongyangwang genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm AT wenjunwang genomewideassociationstudythatidentifiesmolecularmarkerswithfreezingresistanceindurocboarsperm |