Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene
African swine fever virus (ASFV) causes a highly contagious and lethal hemorrhagic disease and significantly threatens the pig industry. There is no commercially effective vaccine available currently, making the detection of ASFV critical for control and prevention. Previously, we established the CR...
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2025-06-01
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| Online Access: | https://www.mdpi.com/2076-2615/15/13/1902 |
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| author | Hongjian Han Desheng Zhang Weilin Hao Anjing Liu Nengwen Xia Meng Cui Jia Luo Sen Jiang Wanglong Zheng Nanhua Chen Jinguo Gu Jianfa Bai Jianzhong Zhu |
| author_facet | Hongjian Han Desheng Zhang Weilin Hao Anjing Liu Nengwen Xia Meng Cui Jia Luo Sen Jiang Wanglong Zheng Nanhua Chen Jinguo Gu Jianfa Bai Jianzhong Zhu |
| author_sort | Hongjian Han |
| collection | DOAJ |
| description | African swine fever virus (ASFV) causes a highly contagious and lethal hemorrhagic disease and significantly threatens the pig industry. There is no commercially effective vaccine available currently, making the detection of ASFV critical for control and prevention. Previously, we established the CRISPR-LbCas12a and LwCRSIRP-Cas13a visual detections of ASFV, separately, targeting the structural p17 gene D117L. In this study, we performed the parallel detections of ASFV based on the conserved viral protease gene S273R using CRISPR-LbCas12a and CRISPR-LbuCas13a systems. Our results showed that both systems are able to specifically detect ASFV as low as two copies of the S273R gene, and effectively detect clinical samples with minimal DNA purification. The work promotes CRISPR-Cas systems for the application of on-site detection in the field. |
| format | Article |
| id | doaj-art-e26d883ac9334fe4a437507eb7900d83 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-e26d883ac9334fe4a437507eb7900d832025-08-20T03:28:32ZengMDPI AGAnimals2076-26152025-06-011513190210.3390/ani15131902Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R GeneHongjian Han0Desheng Zhang1Weilin Hao2Anjing Liu3Nengwen Xia4Meng Cui5Jia Luo6Sen Jiang7Wanglong Zheng8Nanhua Chen9Jinguo Gu10Jianfa Bai11Jianzhong Zhu12College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaKangnongmu Animal Husbandry Ecological Farm, Yancheng 224025, ChinaKansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS 66506, USACollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaAfrican swine fever virus (ASFV) causes a highly contagious and lethal hemorrhagic disease and significantly threatens the pig industry. There is no commercially effective vaccine available currently, making the detection of ASFV critical for control and prevention. Previously, we established the CRISPR-LbCas12a and LwCRSIRP-Cas13a visual detections of ASFV, separately, targeting the structural p17 gene D117L. In this study, we performed the parallel detections of ASFV based on the conserved viral protease gene S273R using CRISPR-LbCas12a and CRISPR-LbuCas13a systems. Our results showed that both systems are able to specifically detect ASFV as low as two copies of the S273R gene, and effectively detect clinical samples with minimal DNA purification. The work promotes CRISPR-Cas systems for the application of on-site detection in the field.https://www.mdpi.com/2076-2615/15/13/1902ASFVdetectionCRISPR-Cas12aCRISPR-Cas13aS273R gene |
| spellingShingle | Hongjian Han Desheng Zhang Weilin Hao Anjing Liu Nengwen Xia Meng Cui Jia Luo Sen Jiang Wanglong Zheng Nanhua Chen Jinguo Gu Jianfa Bai Jianzhong Zhu Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene Animals ASFV detection CRISPR-Cas12a CRISPR-Cas13a S273R gene |
| title | Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene |
| title_full | Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene |
| title_fullStr | Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene |
| title_full_unstemmed | Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene |
| title_short | Parallel and Visual Detections of ASFV by CRISPR-Cas12a and CRISPR-Cas13a Systems Targeting the Viral S273R Gene |
| title_sort | parallel and visual detections of asfv by crispr cas12a and crispr cas13a systems targeting the viral s273r gene |
| topic | ASFV detection CRISPR-Cas12a CRISPR-Cas13a S273R gene |
| url | https://www.mdpi.com/2076-2615/15/13/1902 |
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