Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot
Ripe rot (caused by <i>Colletotrichum</i> species) severely compromises the yield and quality of grapes. Biocontrol approaches, such as antagonistic bacteria, represent an effective strategy to prevent and control grape ripe rot. In this study, 325 strains of <i>Bacillus</i>...
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2025-07-01
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| author | Xin-Qiao Du Yi-Lu Yang Jiang Lu |
| author_facet | Xin-Qiao Du Yi-Lu Yang Jiang Lu |
| author_sort | Xin-Qiao Du |
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| description | Ripe rot (caused by <i>Colletotrichum</i> species) severely compromises the yield and quality of grapes. Biocontrol approaches, such as antagonistic bacteria, represent an effective strategy to prevent and control grape ripe rot. In this study, 325 strains of <i>Bacillus</i> species were isolated from the rhizosphere soil of healthy grapevine plants. Among them, two strains, LJBA01 and LJBV047, exhibit strong antagonistic effects against <i>C. viniferum</i>, suggesting their potential role as biocontrol agents against grape ripe rot. Treatment with the fermentation broth of these strains significantly reduced disease incidence and lesion diameter in infected grape berries. Whole genome sequencing, combined with morphological characterization and 16S rRNA gene sequence, confirmed LJBA01 as <i>Bacillus amyloliquefaciens</i> and LJBV047 as <i>Bacillus velezensis</i>. Furthermore, GC-MS analysis of volatile compounds in fermentation broth from four strains (LJBA01, LJBV047, LJBS06, and LJBS17) identified 29 potential antimicrobial components. Among these, 2-nonanone and 2-decanol demonstrated highly significant inhibitory effects on <i>C. viniferum</i>. Overall, our research confirmed the potential value of two <i>Bacillus</i> strains as biocontrol bacteria against grape ripe rot. |
| format | Article |
| id | doaj-art-e26c4e00e7df42ae87de13bcdc08171a |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-e26c4e00e7df42ae87de13bcdc08171a2025-08-20T03:58:26ZengMDPI AGHorticulturae2311-75242025-07-0111780210.3390/horticulturae11070802Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe RotXin-Qiao Du0Yi-Lu Yang1Jiang Lu2School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaRipe rot (caused by <i>Colletotrichum</i> species) severely compromises the yield and quality of grapes. Biocontrol approaches, such as antagonistic bacteria, represent an effective strategy to prevent and control grape ripe rot. In this study, 325 strains of <i>Bacillus</i> species were isolated from the rhizosphere soil of healthy grapevine plants. Among them, two strains, LJBA01 and LJBV047, exhibit strong antagonistic effects against <i>C. viniferum</i>, suggesting their potential role as biocontrol agents against grape ripe rot. Treatment with the fermentation broth of these strains significantly reduced disease incidence and lesion diameter in infected grape berries. Whole genome sequencing, combined with morphological characterization and 16S rRNA gene sequence, confirmed LJBA01 as <i>Bacillus amyloliquefaciens</i> and LJBV047 as <i>Bacillus velezensis</i>. Furthermore, GC-MS analysis of volatile compounds in fermentation broth from four strains (LJBA01, LJBV047, LJBS06, and LJBS17) identified 29 potential antimicrobial components. Among these, 2-nonanone and 2-decanol demonstrated highly significant inhibitory effects on <i>C. viniferum</i>. Overall, our research confirmed the potential value of two <i>Bacillus</i> strains as biocontrol bacteria against grape ripe rot.https://www.mdpi.com/2311-7524/11/7/802grape ripe rot<i>Colletotrichum</i><i>Bacillus</i>biocontrolmetabolites |
| spellingShingle | Xin-Qiao Du Yi-Lu Yang Jiang Lu Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot Horticulturae grape ripe rot <i>Colletotrichum</i> <i>Bacillus</i> biocontrol metabolites |
| title | Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot |
| title_full | Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot |
| title_fullStr | Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot |
| title_full_unstemmed | Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot |
| title_short | Screening and Characterization of Biocontrol <i>Bacillus</i> Strains Against <i>Colletotrichum</i> Causing Grape Ripe Rot |
| title_sort | screening and characterization of biocontrol i bacillus i strains against i colletotrichum i causing grape ripe rot |
| topic | grape ripe rot <i>Colletotrichum</i> <i>Bacillus</i> biocontrol metabolites |
| url | https://www.mdpi.com/2311-7524/11/7/802 |
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