High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens
Background. Bacteria possessing extended-spectrum beta-lactamase (ESBL), especially E. coli and Klebsiella species, are problematic, particularly in hospitalized patients. Poultry meat vendors are at risk of carrying ESBL-producing bacteria when processing and handling meat products in an unhygienic...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/6653993 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850167200803979264 |
|---|---|
| author | Lutengano W. Mwanginde Mtebe Majigo Debora C. Kajeguka Agricola Joachim |
| author_facet | Lutengano W. Mwanginde Mtebe Majigo Debora C. Kajeguka Agricola Joachim |
| author_sort | Lutengano W. Mwanginde |
| collection | DOAJ |
| description | Background. Bacteria possessing extended-spectrum beta-lactamase (ESBL), especially E. coli and Klebsiella species, are problematic, particularly in hospitalized patients. Poultry meat vendors are at risk of carrying ESBL-producing bacteria when processing and handling meat products in an unhygienic environment. There is limited information on the carriage rate of ESBL-producing pathogens among poultry meat vendors that necessitated the conduction of the study. Method. A cross-sectional study was conducted among poultry meat vendors in Dar es Salaam, Tanzania. Participants provided rectal swabs in transport media upon instruction. The primary isolation of ESBL-producing bacteria was carried out using MacConkey agar supplemented with ceftazidime. Identification of isolates relied on conventional methods. Double-disk synergy was the method used to confirm ESBL-producing isolates. We performed descriptive statistics using Statistical Package for Social Sciences version 23. A p value < 0.05 was considered statistically significant. Results. A total of 300 participants were recruited from five districts, with a mean age of 27.2 ± 6.7 years. The majority was male (67.3%), and 74.7% worked as poultry meat vendors for more than one year. Out of 300 participants, 107 (35.7%) had confirmed ESBL-producing E. coli and Klebsiella spp. The majority of confirmed ESBL-producing isolates was E. coli (78.5%). Participants from Ubungo District had significantly higher carriage of ESBL-producing Escherichia coli and Klebsiella spp. (48.0%, 95% CI: 34.8–47.7) than Temeke District (21.4%, 95% CI: 13.4–32.4). Only 28.0% of participants had access to latrines at the workplace, and all working areas lacked access to running water. Conclusion. The study revealed a relatively high fecal carriage rate of ESBL-producing E. coli and Klebsiella spp. among poultry meat vendors. Poor working environments and hygienic practices are risks for spread of these multidrug-resitant pathogens. |
| format | Article |
| id | doaj-art-e26b666343434e7aba644c13b6b634b8 |
| institution | OA Journals |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-e26b666343434e7aba644c13b6b634b82025-08-20T02:21:14ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/66539936653993High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant PathogensLutengano W. Mwanginde0Mtebe Majigo1Debora C. Kajeguka2Agricola Joachim3Department of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, P. O. Box 65001, Dar es Salaam, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, P. O. Box 65001, Dar es Salaam, TanzaniaFaculty of Medicine, Kilimanjaro Christian Medical University College, P. O. Box 2240, Moshi Kilimanjaro, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, P. O. Box 65001, Dar es Salaam, TanzaniaBackground. Bacteria possessing extended-spectrum beta-lactamase (ESBL), especially E. coli and Klebsiella species, are problematic, particularly in hospitalized patients. Poultry meat vendors are at risk of carrying ESBL-producing bacteria when processing and handling meat products in an unhygienic environment. There is limited information on the carriage rate of ESBL-producing pathogens among poultry meat vendors that necessitated the conduction of the study. Method. A cross-sectional study was conducted among poultry meat vendors in Dar es Salaam, Tanzania. Participants provided rectal swabs in transport media upon instruction. The primary isolation of ESBL-producing bacteria was carried out using MacConkey agar supplemented with ceftazidime. Identification of isolates relied on conventional methods. Double-disk synergy was the method used to confirm ESBL-producing isolates. We performed descriptive statistics using Statistical Package for Social Sciences version 23. A p value < 0.05 was considered statistically significant. Results. A total of 300 participants were recruited from five districts, with a mean age of 27.2 ± 6.7 years. The majority was male (67.3%), and 74.7% worked as poultry meat vendors for more than one year. Out of 300 participants, 107 (35.7%) had confirmed ESBL-producing E. coli and Klebsiella spp. The majority of confirmed ESBL-producing isolates was E. coli (78.5%). Participants from Ubungo District had significantly higher carriage of ESBL-producing Escherichia coli and Klebsiella spp. (48.0%, 95% CI: 34.8–47.7) than Temeke District (21.4%, 95% CI: 13.4–32.4). Only 28.0% of participants had access to latrines at the workplace, and all working areas lacked access to running water. Conclusion. The study revealed a relatively high fecal carriage rate of ESBL-producing E. coli and Klebsiella spp. among poultry meat vendors. Poor working environments and hygienic practices are risks for spread of these multidrug-resitant pathogens.http://dx.doi.org/10.1155/2021/6653993 |
| spellingShingle | Lutengano W. Mwanginde Mtebe Majigo Debora C. Kajeguka Agricola Joachim High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens International Journal of Microbiology |
| title | High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens |
| title_full | High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens |
| title_fullStr | High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens |
| title_full_unstemmed | High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens |
| title_short | High Carriage Rate of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella Species among Poultry Meat Vendors in Dar es Salaam: The Urgent Need for Intervention to Prevent the Spread of Multidrug-Resistant Pathogens |
| title_sort | high carriage rate of extended spectrum β lactamase producing escherichia coli and klebsiella species among poultry meat vendors in dar es salaam the urgent need for intervention to prevent the spread of multidrug resistant pathogens |
| url | http://dx.doi.org/10.1155/2021/6653993 |
| work_keys_str_mv | AT lutenganowmwanginde highcarriagerateofextendedspectrumblactamaseproducingescherichiacoliandklebsiellaspeciesamongpoultrymeatvendorsindaressalaamtheurgentneedforinterventiontopreventthespreadofmultidrugresistantpathogens AT mtebemajigo highcarriagerateofextendedspectrumblactamaseproducingescherichiacoliandklebsiellaspeciesamongpoultrymeatvendorsindaressalaamtheurgentneedforinterventiontopreventthespreadofmultidrugresistantpathogens AT deborackajeguka highcarriagerateofextendedspectrumblactamaseproducingescherichiacoliandklebsiellaspeciesamongpoultrymeatvendorsindaressalaamtheurgentneedforinterventiontopreventthespreadofmultidrugresistantpathogens AT agricolajoachim highcarriagerateofextendedspectrumblactamaseproducingescherichiacoliandklebsiellaspeciesamongpoultrymeatvendorsindaressalaamtheurgentneedforinterventiontopreventthespreadofmultidrugresistantpathogens |