Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells
Reproductive cells play a crucial role in transmitting genetic and epigenetic information from one generation to the next. Oocytes are fundamental to reproduction but human oocytes are difficult to obtain for clinical and research purposes because of ethical restrictions. However, in vitro induction...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2025.1515253/full |
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| author | Mingxin Tian Mingxin Tian Meixiang Zhang Meixiang Zhang |
| author_facet | Mingxin Tian Mingxin Tian Meixiang Zhang Meixiang Zhang |
| author_sort | Mingxin Tian |
| collection | DOAJ |
| description | Reproductive cells play a crucial role in transmitting genetic and epigenetic information from one generation to the next. Oocytes are fundamental to reproduction but human oocytes are difficult to obtain for clinical and research purposes because of ethical restrictions. However, in vitro induction systems have been established to differentiate pluripotent stem cells (PSCs) into human primordial germ cells (hPGCs). These induced hPGCs are referred to as hPGC-like cells. The discovery of ovarian stem cells (OSCs) also opened up a new avenue for studying the development of germline stem cells. In this review, we discuss the latest advances in the development of oocytes in vivo and in vitro, involving PSC-derived PGCs and ovary-isolated OSCs. Specifically, we focus on induction methods and differentiation mechanisms and discuss the associated technical challenges and future directions. |
| format | Article |
| id | doaj-art-e26a3eacd69e487bb3d735bf0f070b11 |
| institution | DOAJ |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj-art-e26a3eacd69e487bb3d735bf0f070b112025-08-20T02:58:11ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-08-011610.3389/fendo.2025.15152531515253Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cellsMingxin Tian0Mingxin Tian1Meixiang Zhang2Meixiang Zhang3Center for Reproductive Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaCenter for Reproductive Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Reproduction and Genetics, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaReproductive cells play a crucial role in transmitting genetic and epigenetic information from one generation to the next. Oocytes are fundamental to reproduction but human oocytes are difficult to obtain for clinical and research purposes because of ethical restrictions. However, in vitro induction systems have been established to differentiate pluripotent stem cells (PSCs) into human primordial germ cells (hPGCs). These induced hPGCs are referred to as hPGC-like cells. The discovery of ovarian stem cells (OSCs) also opened up a new avenue for studying the development of germline stem cells. In this review, we discuss the latest advances in the development of oocytes in vivo and in vitro, involving PSC-derived PGCs and ovary-isolated OSCs. Specifically, we focus on induction methods and differentiation mechanisms and discuss the associated technical challenges and future directions.https://www.frontiersin.org/articles/10.3389/fendo.2025.1515253/fullprimordial germ cell-like cellsovarian stem cellspluripotent stem cellsoogenesisoocyte |
| spellingShingle | Mingxin Tian Mingxin Tian Meixiang Zhang Meixiang Zhang Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells Frontiers in Endocrinology primordial germ cell-like cells ovarian stem cells pluripotent stem cells oogenesis oocyte |
| title | Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells |
| title_full | Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells |
| title_fullStr | Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells |
| title_full_unstemmed | Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells |
| title_short | Advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells |
| title_sort | advances in in vitro oocyte generation from pluripotent stem cells and ovarian stem cells |
| topic | primordial germ cell-like cells ovarian stem cells pluripotent stem cells oogenesis oocyte |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2025.1515253/full |
| work_keys_str_mv | AT mingxintian advancesininvitrooocytegenerationfrompluripotentstemcellsandovarianstemcells AT mingxintian advancesininvitrooocytegenerationfrompluripotentstemcellsandovarianstemcells AT meixiangzhang advancesininvitrooocytegenerationfrompluripotentstemcellsandovarianstemcells AT meixiangzhang advancesininvitrooocytegenerationfrompluripotentstemcellsandovarianstemcells |