Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)

Chlorpyrifos (CHP) contamination affects agricultural land and poses significant risks to plants and humans. Chitosan-oligosaccharide (COS) enhances plant resilience under stress and boosts the activity of enzymes metabolizing exogenous substances. This study aimed to explore the potential and mecha...

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Main Authors: Jingchong Li, Wende Zheng, Jingkun Li, Komelle Askari, Zhixiang Tian, Aohui Han, Runqiang Liu
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325004828
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author Jingchong Li
Wende Zheng
Jingkun Li
Komelle Askari
Zhixiang Tian
Aohui Han
Runqiang Liu
author_facet Jingchong Li
Wende Zheng
Jingkun Li
Komelle Askari
Zhixiang Tian
Aohui Han
Runqiang Liu
author_sort Jingchong Li
collection DOAJ
description Chlorpyrifos (CHP) contamination affects agricultural land and poses significant risks to plants and humans. Chitosan-oligosaccharide (COS) enhances plant resilience under stress and boosts the activity of enzymes metabolizing exogenous substances. This study aimed to explore the potential and mechanism of COS in mitigating CHP phytotoxicity and reducing CHP accumulation through both pot and field experiments. The results indicated that CHP exposure caused oxidative stress and decreased photosynthesis by 18.5 % in wheat. COS up-regulated the expression of antioxidant enzyme genes in CHP-stressed plants, resulting in a 12.1 %-29.4 % increase in antioxidant enzyme activity, which resulted in an 11.3 %-12.8 % reduction in reactive oxygen species (ROS) and an 11.5 %-14.7 % reduction in malondialdehyde (MDA) content in leaves and roots, respectively. Additionally, COS increased chlorophyll content by 6.6 % by regulating genes related to chlorophyll metabolism, enhancing photosynthesis by 13.6 %. COS also reduced CHP uptake and accelerated its metabolism by upregulating CYP450, GST, and lignin biosynthesis-related genes. Wheat treated with COS exhibited a 26.7 %-28.7 % reduction in grains' CHP content, resulting in a lower health risk index (HRI). These findings provide novel insights into the potential of COS in alleviating CHP phytotoxicity and reducing its accumulation.
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publishDate 2025-04-01
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spelling doaj-art-e264dbd64f144a85bf3d203eb62997902025-08-20T03:17:35ZengElsevierEcotoxicology and Environmental Safety0147-65132025-04-0129511814610.1016/j.ecoenv.2025.118146Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)Jingchong Li0Wende Zheng1Jingkun Li2Komelle Askari3Zhixiang Tian4Aohui Han5Runqiang Liu6Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection and School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China; University of the Chinese Academy of Sciences, Beijing 100049, ChinaUniversity of the Chinese Academy of Sciences, Beijing 100049, ChinaHenan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection and School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, ChinaCollege of Soil and Water Conservation Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, ChinaHenan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection and School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, ChinaHenan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection and School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, ChinaHenan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection and School of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China; Corresponding author.Chlorpyrifos (CHP) contamination affects agricultural land and poses significant risks to plants and humans. Chitosan-oligosaccharide (COS) enhances plant resilience under stress and boosts the activity of enzymes metabolizing exogenous substances. This study aimed to explore the potential and mechanism of COS in mitigating CHP phytotoxicity and reducing CHP accumulation through both pot and field experiments. The results indicated that CHP exposure caused oxidative stress and decreased photosynthesis by 18.5 % in wheat. COS up-regulated the expression of antioxidant enzyme genes in CHP-stressed plants, resulting in a 12.1 %-29.4 % increase in antioxidant enzyme activity, which resulted in an 11.3 %-12.8 % reduction in reactive oxygen species (ROS) and an 11.5 %-14.7 % reduction in malondialdehyde (MDA) content in leaves and roots, respectively. Additionally, COS increased chlorophyll content by 6.6 % by regulating genes related to chlorophyll metabolism, enhancing photosynthesis by 13.6 %. COS also reduced CHP uptake and accelerated its metabolism by upregulating CYP450, GST, and lignin biosynthesis-related genes. Wheat treated with COS exhibited a 26.7 %-28.7 % reduction in grains' CHP content, resulting in a lower health risk index (HRI). These findings provide novel insights into the potential of COS in alleviating CHP phytotoxicity and reducing its accumulation.http://www.sciencedirect.com/science/article/pii/S0147651325004828InsecticidePlant growth regulatorOxidative damageDaily pesticide intakeHealth risk index
spellingShingle Jingchong Li
Wende Zheng
Jingkun Li
Komelle Askari
Zhixiang Tian
Aohui Han
Runqiang Liu
Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)
Ecotoxicology and Environmental Safety
Insecticide
Plant growth regulator
Oxidative damage
Daily pesticide intake
Health risk index
title Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)
title_full Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)
title_fullStr Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)
title_full_unstemmed Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)
title_short Chitosan-oligosaccharide alleviates chlorpyrifos-induced biochemical and developmental toxicity and reduces its accumulation in wheat (Triticum aestivum L.)
title_sort chitosan oligosaccharide alleviates chlorpyrifos induced biochemical and developmental toxicity and reduces its accumulation in wheat triticum aestivum l
topic Insecticide
Plant growth regulator
Oxidative damage
Daily pesticide intake
Health risk index
url http://www.sciencedirect.com/science/article/pii/S0147651325004828
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