Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs
Chondrogenic differentiating mesenchymal stem cells (MSCs) are mimicking embryonal endochondral ossification and become hypertrophic. BMP (bone morphogenetic protein) and Activin Membrane Bound Inhibitor (BAMBI) is a pseudoreceptor that regulates the activity of transforming growth factor-β (TGF-β)...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2685147 |
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| author | Christian G. Pfeifer Alexandra Karl Arne Berner Johannes Zellner Paul Schmitz Markus Loibl Matthias Koch Peter Angele Michael Nerlich Michael B. Mueller |
| author_facet | Christian G. Pfeifer Alexandra Karl Arne Berner Johannes Zellner Paul Schmitz Markus Loibl Matthias Koch Peter Angele Michael Nerlich Michael B. Mueller |
| author_sort | Christian G. Pfeifer |
| collection | DOAJ |
| description | Chondrogenic differentiating mesenchymal stem cells (MSCs) are mimicking embryonal endochondral ossification and become hypertrophic. BMP (bone morphogenetic protein) and Activin Membrane Bound Inhibitor (BAMBI) is a pseudoreceptor that regulates the activity of transforming growth factor-β (TGF-β) and BMP signalling during chondrogenesis. Both TGF-β and BMP signalling are regulators of chondrogenic cell differentiation. Human bone marrow derived MSCs were chondrogenically predifferentiated in aggregate culture for 14 days. Thereafter, one group was subjected to hypertrophy enhancing media conditions while controls were kept in chondrogenic medium until day 28. Histological evaluation, gene expression by PCR, and Western blot analysis were carried out at days 1, 3, 7, 14, 17, 21, and 28. A subset of cultures was treated with the BMP inhibitor Noggin to test for BMP dependent expression of BAMBI. Hypertrophic differentiated pellets showed larger cells with increased collagen 10 and alkaline phosphatase staining. There was significantly increased expression of BAMBI on gene expression and protein level in hypertrophic cultures compared to the chondrogenic control and increased BMP4 gene expression. Immunohistochemistry showed intense staining of BAMBI in hypertrophic cells. BAMBI expression was dose-dependently downregulated by Noggin. The pseudoreceptor BAMBI is upregulated upon enhancement of hypertrophy in MSC chondrogenic differentiation by a BMP dependent mechanism. |
| format | Article |
| id | doaj-art-e2644eaa3e004610b86d2c661efe664e |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e2644eaa3e004610b86d2c661efe664e2025-08-20T02:21:14ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/26851472685147Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCsChristian G. Pfeifer0Alexandra Karl1Arne Berner2Johannes Zellner3Paul Schmitz4Markus Loibl5Matthias Koch6Peter Angele7Michael Nerlich8Michael B. Mueller9Department of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyDepartment of Trauma and Orthopaedic Surgery, University Clinic Regensburg, Regensburg, GermanyChondrogenic differentiating mesenchymal stem cells (MSCs) are mimicking embryonal endochondral ossification and become hypertrophic. BMP (bone morphogenetic protein) and Activin Membrane Bound Inhibitor (BAMBI) is a pseudoreceptor that regulates the activity of transforming growth factor-β (TGF-β) and BMP signalling during chondrogenesis. Both TGF-β and BMP signalling are regulators of chondrogenic cell differentiation. Human bone marrow derived MSCs were chondrogenically predifferentiated in aggregate culture for 14 days. Thereafter, one group was subjected to hypertrophy enhancing media conditions while controls were kept in chondrogenic medium until day 28. Histological evaluation, gene expression by PCR, and Western blot analysis were carried out at days 1, 3, 7, 14, 17, 21, and 28. A subset of cultures was treated with the BMP inhibitor Noggin to test for BMP dependent expression of BAMBI. Hypertrophic differentiated pellets showed larger cells with increased collagen 10 and alkaline phosphatase staining. There was significantly increased expression of BAMBI on gene expression and protein level in hypertrophic cultures compared to the chondrogenic control and increased BMP4 gene expression. Immunohistochemistry showed intense staining of BAMBI in hypertrophic cells. BAMBI expression was dose-dependently downregulated by Noggin. The pseudoreceptor BAMBI is upregulated upon enhancement of hypertrophy in MSC chondrogenic differentiation by a BMP dependent mechanism.http://dx.doi.org/10.1155/2016/2685147 |
| spellingShingle | Christian G. Pfeifer Alexandra Karl Arne Berner Johannes Zellner Paul Schmitz Markus Loibl Matthias Koch Peter Angele Michael Nerlich Michael B. Mueller Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs Stem Cells International |
| title | Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs |
| title_full | Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs |
| title_fullStr | Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs |
| title_full_unstemmed | Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs |
| title_short | Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs |
| title_sort | expression of bmp and actin membrane bound inhibitor is increased during terminal differentiation of mscs |
| url | http://dx.doi.org/10.1155/2016/2685147 |
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