Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale
DNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of other applications in bionanotechnology. On the nanoscale, these entities represent unique challenges from a physicochemical characterization perspective. Here, nucleic acid conjugates...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2012/954601 |
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| author | Robert K. DeLong Lisa Cillessen Chris Reynolds Adam Wanekaya Tiffany Severs Kartik Ghosh Michael Fisher Stephanie Barber John Black Ashley Schaeffer Kristin J. Flores |
| author_facet | Robert K. DeLong Lisa Cillessen Chris Reynolds Adam Wanekaya Tiffany Severs Kartik Ghosh Michael Fisher Stephanie Barber John Black Ashley Schaeffer Kristin J. Flores |
| author_sort | Robert K. DeLong |
| collection | DOAJ |
| description | DNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of other applications in bionanotechnology. On the nanoscale, these entities represent unique challenges from a physicochemical characterization perspective. Here, nucleic acid conjugates with protamine and gold nanoparticles (GNP) were characterized comparatively in the nanorange of concentration by UV/Vis NanoDrop spectroscopy, fluorimetry, and gel electrophoresis. Given the intense interest in splice-site switching oligomers (SSOs), we utilized a human tumor cell culture system (HeLa pLuc-705), in which SSO-directed splicing repair upregulates luciferase expression, in order to investigate bioactivity of the bionanoconjugates. Process parameters important for bioactivity were investigated, and the bimolecular nanoconjugates were confirmed by shifts in the dynamic laser light scatter (DLLS), UV/Vis spectrum, gel electrophoresis, or sedimentation pattern. The data presented herein may be useful in the future development of pharmaceutical and biotechnology formulations, processes, and analyses concerning protein, DNA, or RNA bionanoconjugates. |
| format | Article |
| id | doaj-art-e26172e1c91543b0946d71b1726179ef |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-e26172e1c91543b0946d71b1726179ef2025-02-03T05:54:04ZengWileyJournal of Nanotechnology1687-95031687-95112012-01-01201210.1155/2012/954601954601Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the NanoscaleRobert K. DeLong0Lisa Cillessen1Chris Reynolds2Adam Wanekaya3Tiffany Severs4Kartik Ghosh5Michael Fisher6Stephanie Barber7John Black8Ashley Schaeffer9Kristin J. Flores10Cell and Molecular Biology Program, Department of Biomedical Sciences, Missouri State University, Springfield, MO 65897, USACollege of Pharmacy, The Ohio State University, Columbus, OH 43210, USADepartment of Chemistry, Missouri State University, Springfield, MO 65897, USADepartment of Chemistry, Missouri State University, Springfield, MO 65897, USADepartment of Chemistry, Missouri State University, Springfield, MO 65897, USADepartment of Physics Astronomy and Materials Science, Missouri State University, Springfield, MO 65897, USASchool of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USAGeorge Warren Brown School of Social Work, Washington University in St. Louis, St. Louis, MO 63130, USASchool of Medicine, University of Pittsburgh, Pittsburg, PA 15261, USACell and Molecular Biology Program, Department of Biomedical Sciences, Missouri State University, Springfield, MO 65897, USACell and Molecular Biology Program, Department of Biomedical Sciences, Missouri State University, Springfield, MO 65897, USADNA and RNA micro- and nanoparticles are increasingly being used for gene and siRNA drug delivery and a variety of other applications in bionanotechnology. On the nanoscale, these entities represent unique challenges from a physicochemical characterization perspective. Here, nucleic acid conjugates with protamine and gold nanoparticles (GNP) were characterized comparatively in the nanorange of concentration by UV/Vis NanoDrop spectroscopy, fluorimetry, and gel electrophoresis. Given the intense interest in splice-site switching oligomers (SSOs), we utilized a human tumor cell culture system (HeLa pLuc-705), in which SSO-directed splicing repair upregulates luciferase expression, in order to investigate bioactivity of the bionanoconjugates. Process parameters important for bioactivity were investigated, and the bimolecular nanoconjugates were confirmed by shifts in the dynamic laser light scatter (DLLS), UV/Vis spectrum, gel electrophoresis, or sedimentation pattern. The data presented herein may be useful in the future development of pharmaceutical and biotechnology formulations, processes, and analyses concerning protein, DNA, or RNA bionanoconjugates.http://dx.doi.org/10.1155/2012/954601 |
| spellingShingle | Robert K. DeLong Lisa Cillessen Chris Reynolds Adam Wanekaya Tiffany Severs Kartik Ghosh Michael Fisher Stephanie Barber John Black Ashley Schaeffer Kristin J. Flores Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale Journal of Nanotechnology |
| title | Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale |
| title_full | Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale |
| title_fullStr | Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale |
| title_full_unstemmed | Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale |
| title_short | Biomolecular Triconjugates Formed between Gold, Protamine, and Nucleic Acid: Comparative Characterization on the Nanoscale |
| title_sort | biomolecular triconjugates formed between gold protamine and nucleic acid comparative characterization on the nanoscale |
| url | http://dx.doi.org/10.1155/2012/954601 |
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