Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy
Synchrotron sourced Fourier transform infrared (SS FTIR) microspectroscopy was employed to investigate the biological effects on the neuron-like pheochromocytoma (PC 12) cells after exposure to synchrotron sourced terahertz (SS THz) radiation. Over 10 min of exposure, the PC 12 cells received a tota...
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International Union of Crystallography
2025-01-01
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| Series: | Journal of Synchrotron Radiation |
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| Online Access: | https://journals.iucr.org/paper?S1600577524010944 |
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| author | Palalle G. Tharushi Perera Jitraporn Vongsvivut Denver Linklater Zoltan Vilagosh Dominique Appadoo The Hong Phong Nguyen Mark J. Tobin Rodney Croft Elena P. Ivanova |
| author_facet | Palalle G. Tharushi Perera Jitraporn Vongsvivut Denver Linklater Zoltan Vilagosh Dominique Appadoo The Hong Phong Nguyen Mark J. Tobin Rodney Croft Elena P. Ivanova |
| author_sort | Palalle G. Tharushi Perera |
| collection | DOAJ |
| description | Synchrotron sourced Fourier transform infrared (SS FTIR) microspectroscopy was employed to investigate the biological effects on the neuron-like pheochromocytoma (PC 12) cells after exposure to synchrotron sourced terahertz (SS THz) radiation. Over 10 min of exposure, the PC 12 cells received a total energy of 600 J m2, with a total incident power density of ∼1.0 W m−2 (0.10 mW cm−2) at the beam extraction port (BEP) of the THz beamline at the Australian Synchrotron. To investigate the metabolic response of PC 12 cells after synchrotron THz radiation exposure, we utilized the FTIR microscope at the Infrared Microspectroscopy IRM beamline, which offers high photon flux and diffraction-limited spatial resolution enabling the detection of functional group variations in biological molecules at a single-cell level. Principal component analysis (PCA) based on the SS FTIR spectral data revealed a distinct separation of SS THz-exposed and control (non-exposed) cells. According to the PCA loadings, the key changes in the exposed cells involved lipid and protein compositions as indicated by the stretching vibrations of CH2/CH3 groups and amide I/II bands, respectively. An increase in lipids, such as cholesterol, or notable changes in their compositions and in some protein secondary structures were observed in the SS THz-exposed cells. The PCA analysis further suggests that PC 12 cells might maintain cell membrane stability after SS THz irradiation through higher volumes of cholesterol and cell morphology via regulation of the synthesis of cytoskeleton proteins such as actin-related proteins. The outcome of this study re-emphasized the exceptional SS FTIR capability to perform single-cell analysis directly, providing (i) unique biological information on cell variability within the population as well as between different groups, and (ii) evidence of molecular changes in the exposed cells that could lead to a deeper understanding of the effect of THz exposure at a single-cell level. |
| format | Article |
| id | doaj-art-e26057070ef149329b5e1673ec21d522 |
| institution | Kabale University |
| issn | 1600-5775 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj-art-e26057070ef149329b5e1673ec21d5222025-01-07T14:26:38ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752025-01-0132115516110.1107/S1600577524010944ing5002Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopyPalalle G. Tharushi Perera0Jitraporn Vongsvivut1Denver Linklater2Zoltan Vilagosh3Dominique Appadoo4The Hong Phong Nguyen5Mark J. Tobin6Rodney Croft7Elena P. Ivanova8School of Science, RMIT University, 2476, Melbourne, Victoria 3001, AustraliaIR Microspectroscopy (IRM) Beamline, ANSTO-Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, AustraliaSchool of Science, RMIT University, 2476, Melbourne, Victoria 3001, AustraliaSchool of Science, RMIT University, 2476, Melbourne, Victoria 3001, AustraliaTHz Beamline, Australian Synchrotron, 800 Blackburn Road, Melbourne, Victoria 3168, AustraliaSchool of Science, RMIT University, 2476, Melbourne, Victoria 3001, AustraliaIR Microspectroscopy (IRM) Beamline, ANSTO-Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, AustraliaSchool of Psychology, Illawara and Medical Research Institute, University of Wollongong, Wollongong, New South Wales 2522, AustraliaSchool of Science, RMIT University, 2476, Melbourne, Victoria 3001, AustraliaSynchrotron sourced Fourier transform infrared (SS FTIR) microspectroscopy was employed to investigate the biological effects on the neuron-like pheochromocytoma (PC 12) cells after exposure to synchrotron sourced terahertz (SS THz) radiation. Over 10 min of exposure, the PC 12 cells received a total energy of 600 J m2, with a total incident power density of ∼1.0 W m−2 (0.10 mW cm−2) at the beam extraction port (BEP) of the THz beamline at the Australian Synchrotron. To investigate the metabolic response of PC 12 cells after synchrotron THz radiation exposure, we utilized the FTIR microscope at the Infrared Microspectroscopy IRM beamline, which offers high photon flux and diffraction-limited spatial resolution enabling the detection of functional group variations in biological molecules at a single-cell level. Principal component analysis (PCA) based on the SS FTIR spectral data revealed a distinct separation of SS THz-exposed and control (non-exposed) cells. According to the PCA loadings, the key changes in the exposed cells involved lipid and protein compositions as indicated by the stretching vibrations of CH2/CH3 groups and amide I/II bands, respectively. An increase in lipids, such as cholesterol, or notable changes in their compositions and in some protein secondary structures were observed in the SS THz-exposed cells. The PCA analysis further suggests that PC 12 cells might maintain cell membrane stability after SS THz irradiation through higher volumes of cholesterol and cell morphology via regulation of the synthesis of cytoskeleton proteins such as actin-related proteins. The outcome of this study re-emphasized the exceptional SS FTIR capability to perform single-cell analysis directly, providing (i) unique biological information on cell variability within the population as well as between different groups, and (ii) evidence of molecular changes in the exposed cells that could lead to a deeper understanding of the effect of THz exposure at a single-cell level.https://journals.iucr.org/paper?S1600577524010944synchrotron source ftirprincipal component analysispcasynchrotron source thz radiationpc 12 neuronal cellsmembrane permeabilityblebssynchrotron source fourier transform infrared microspectroscopypheochromocytoma cells |
| spellingShingle | Palalle G. Tharushi Perera Jitraporn Vongsvivut Denver Linklater Zoltan Vilagosh Dominique Appadoo The Hong Phong Nguyen Mark J. Tobin Rodney Croft Elena P. Ivanova Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy Journal of Synchrotron Radiation synchrotron source ftir principal component analysis pca synchrotron source thz radiation pc 12 neuronal cells membrane permeability blebs synchrotron source fourier transform infrared microspectroscopy pheochromocytoma cells |
| title | Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy |
| title_full | Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy |
| title_fullStr | Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy |
| title_full_unstemmed | Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy |
| title_short | Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy |
| title_sort | shedding light on biochemical changes in single neuron like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source fourier transform infrared microspectroscopy |
| topic | synchrotron source ftir principal component analysis pca synchrotron source thz radiation pc 12 neuronal cells membrane permeability blebs synchrotron source fourier transform infrared microspectroscopy pheochromocytoma cells |
| url | https://journals.iucr.org/paper?S1600577524010944 |
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