Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model
Liver fibrosis is a common pathological condition that can progress to cirrhosis and hepatocellular carcinoma. One potential treatment involves natural ingredients, such as moringa seed oil (MOSEIL). The proinflammatory cytokines IL-1β and IL-18 play a role in liver fibrosis pathogenesis, and their...
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| Format: | Article |
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/34/bioconf_icolist2024_01001.pdf |
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| author | Susanto Hendra Negara Diajeng Ravika Adil Bangun Melani Inriana Candra Nurfadila Wafiq Azizah Azizah Khulud Rizqi Zahira Hasnawati Malek Nik Ahmad Nizam Nik |
| author_facet | Susanto Hendra Negara Diajeng Ravika Adil Bangun Melani Inriana Candra Nurfadila Wafiq Azizah Azizah Khulud Rizqi Zahira Hasnawati Malek Nik Ahmad Nizam Nik |
| author_sort | Susanto Hendra |
| collection | DOAJ |
| description | Liver fibrosis is a common pathological condition that can progress to cirrhosis and hepatocellular carcinoma. One potential treatment involves natural ingredients, such as moringa seed oil (MOSEIL). The proinflammatory cytokines IL-1β and IL-18 play a role in liver fibrosis pathogenesis, and their increased expression may indicate fibrosis progression. This study examines the effect of MOSEIL on IL-1β and IL-18 gene expression in a liver fibrosis mouse model induced by CCl4 BALB/c mice were treated with CCl4 for one month, followed by CCl4 and MOSEIL in the second month. Gene expression was analyzed using RT-qPCR. The results showed that MOSEIL prevented weight loss in mice and altered liver morphology, including color changes, lump formation, and cell infiltration in histopathology. Additionally, MOSEIL suppressed IL-1β and IL-18 expression, inhibiting fibrosis progression. In conclusion, MOSEIL can reduce fibrosis development by modulating pro-inflammatory gene expression. These findings support its potential use in developing MOSEIL-based treatments for chronic liver disease. |
| format | Article |
| id | doaj-art-e25f1cd2c0d547b69b1fc0f9d3680149 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-e25f1cd2c0d547b69b1fc0f9d36801492025-08-20T03:28:09ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011830100110.1051/bioconf/202518301001bioconf_icolist2024_01001Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis ModelSusanto Hendra0Negara Diajeng Ravika Adil Bangun1Melani Inriana Candra2Nurfadila Wafiq Azizah3Azizah Khulud Rizqi4Zahira Hasnawati5Malek Nik Ahmad Nizam Nik6Departement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri MalangDepartement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri MalangDepartement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri MalangDepartement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri MalangDepartement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri MalangDepartement of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri MalangDepartement of Biosciences, Faculty of Sciences, Universiti Teknologi MalaysiaLiver fibrosis is a common pathological condition that can progress to cirrhosis and hepatocellular carcinoma. One potential treatment involves natural ingredients, such as moringa seed oil (MOSEIL). The proinflammatory cytokines IL-1β and IL-18 play a role in liver fibrosis pathogenesis, and their increased expression may indicate fibrosis progression. This study examines the effect of MOSEIL on IL-1β and IL-18 gene expression in a liver fibrosis mouse model induced by CCl4 BALB/c mice were treated with CCl4 for one month, followed by CCl4 and MOSEIL in the second month. Gene expression was analyzed using RT-qPCR. The results showed that MOSEIL prevented weight loss in mice and altered liver morphology, including color changes, lump formation, and cell infiltration in histopathology. Additionally, MOSEIL suppressed IL-1β and IL-18 expression, inhibiting fibrosis progression. In conclusion, MOSEIL can reduce fibrosis development by modulating pro-inflammatory gene expression. These findings support its potential use in developing MOSEIL-based treatments for chronic liver disease.https://www.bio-conferences.org/articles/bioconf/pdf/2025/34/bioconf_icolist2024_01001.pdf |
| spellingShingle | Susanto Hendra Negara Diajeng Ravika Adil Bangun Melani Inriana Candra Nurfadila Wafiq Azizah Azizah Khulud Rizqi Zahira Hasnawati Malek Nik Ahmad Nizam Nik Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model BIO Web of Conferences |
| title | Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model |
| title_full | Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model |
| title_fullStr | Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model |
| title_full_unstemmed | Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model |
| title_short | Effect of Moringa oleifera Seed Oil on IL-Iβ and IL-18 Gene Expression in Mice (Mus muculus) Liver Fibrosis Model |
| title_sort | effect of moringa oleifera seed oil on il iβ and il 18 gene expression in mice mus muculus liver fibrosis model |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/34/bioconf_icolist2024_01001.pdf |
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