Engineering in vitro vascular microsystems

Abstract Blood vessels are hierarchical microchannels that transport nutrients and oxygen to different tissues and organs, while also eliminating metabolic waste from the body. Disorders of the vascular system impact both physiological and pathological processes. Conventional animal vascular models...

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Main Authors: Qiao Liu, Guoliang Ying, Chenyan Hu, Lingyu Du, Huaiyi Zhang, Zhenye Wang, Hongyan Yue, Ali K. Yetisen, Guixue Wang, Yang Shen, Nan Jiang
Format: Article
Language:English
Published: Nature Publishing Group 2025-05-01
Series:Microsystems & Nanoengineering
Online Access:https://doi.org/10.1038/s41378-025-00956-w
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author Qiao Liu
Guoliang Ying
Chenyan Hu
Lingyu Du
Huaiyi Zhang
Zhenye Wang
Hongyan Yue
Ali K. Yetisen
Guixue Wang
Yang Shen
Nan Jiang
author_facet Qiao Liu
Guoliang Ying
Chenyan Hu
Lingyu Du
Huaiyi Zhang
Zhenye Wang
Hongyan Yue
Ali K. Yetisen
Guixue Wang
Yang Shen
Nan Jiang
author_sort Qiao Liu
collection DOAJ
description Abstract Blood vessels are hierarchical microchannels that transport nutrients and oxygen to different tissues and organs, while also eliminating metabolic waste from the body. Disorders of the vascular system impact both physiological and pathological processes. Conventional animal vascular models are complex, high-cost, time-consuming, and low-validity, which have limited the exploration of effective in vitro vascular microsystems. The morphologies of micro-scaled tubular structures and physiological properties of vascular tissues, including mechanical strength, thrombogenicity, and immunogenicity, can be mimicked in vitro by engineering strategies. This review highlights the state-of-the-art and advanced engineering strategies for in vitro vascular microsystems, covering the domains related to rational designs, manufacturing approaches, supporting materials, and organ-specific cell types. A broad range of biomedical applications of in vitro vascular microsystems are also summarized, including the recent advances in engineered vascularized tissues and organs for physiological and pathological study, drug screening, and personalized medicine. Moreover, the commercialization of in vitro vascular microsystems, the feasibility and limitations of current strategies and commercially available products, as well as perspectives on future directions for exploration, are elaborated. The in vitro modeling of vascular microsystems will facilitate rapid, robust, and efficient analysis in tissue engineering and broader regenerative medicine towards the development of personalized treatment approaches.
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spelling doaj-art-e2586f33b8214019a2673599fa8238bf2025-08-20T01:53:23ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342025-05-0111113610.1038/s41378-025-00956-wEngineering in vitro vascular microsystemsQiao Liu0Guoliang Ying1Chenyan Hu2Lingyu Du3Huaiyi Zhang4Zhenye Wang5Hongyan Yue6Ali K. Yetisen7Guixue Wang8Yang Shen9Nan Jiang10West China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityDepartment of Chemical Engineering, Imperial College LondonJinfeng LaboratoryWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan UniversityAbstract Blood vessels are hierarchical microchannels that transport nutrients and oxygen to different tissues and organs, while also eliminating metabolic waste from the body. Disorders of the vascular system impact both physiological and pathological processes. Conventional animal vascular models are complex, high-cost, time-consuming, and low-validity, which have limited the exploration of effective in vitro vascular microsystems. The morphologies of micro-scaled tubular structures and physiological properties of vascular tissues, including mechanical strength, thrombogenicity, and immunogenicity, can be mimicked in vitro by engineering strategies. This review highlights the state-of-the-art and advanced engineering strategies for in vitro vascular microsystems, covering the domains related to rational designs, manufacturing approaches, supporting materials, and organ-specific cell types. A broad range of biomedical applications of in vitro vascular microsystems are also summarized, including the recent advances in engineered vascularized tissues and organs for physiological and pathological study, drug screening, and personalized medicine. Moreover, the commercialization of in vitro vascular microsystems, the feasibility and limitations of current strategies and commercially available products, as well as perspectives on future directions for exploration, are elaborated. The in vitro modeling of vascular microsystems will facilitate rapid, robust, and efficient analysis in tissue engineering and broader regenerative medicine towards the development of personalized treatment approaches.https://doi.org/10.1038/s41378-025-00956-w
spellingShingle Qiao Liu
Guoliang Ying
Chenyan Hu
Lingyu Du
Huaiyi Zhang
Zhenye Wang
Hongyan Yue
Ali K. Yetisen
Guixue Wang
Yang Shen
Nan Jiang
Engineering in vitro vascular microsystems
Microsystems & Nanoengineering
title Engineering in vitro vascular microsystems
title_full Engineering in vitro vascular microsystems
title_fullStr Engineering in vitro vascular microsystems
title_full_unstemmed Engineering in vitro vascular microsystems
title_short Engineering in vitro vascular microsystems
title_sort engineering in vitro vascular microsystems
url https://doi.org/10.1038/s41378-025-00956-w
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AT zhenyewang engineeringinvitrovascularmicrosystems
AT hongyanyue engineeringinvitrovascularmicrosystems
AT alikyetisen engineeringinvitrovascularmicrosystems
AT guixuewang engineeringinvitrovascularmicrosystems
AT yangshen engineeringinvitrovascularmicrosystems
AT nanjiang engineeringinvitrovascularmicrosystems