A PLIN1 polymorphism is associated with fat production in male emus
The emu (Dromaius novaehollandiae) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin...
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124010915 |
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| author | Yuichi Koshiishi Ryo Takahashi Michiko Murata-Okubo Yuichi Kameyama Kousaku Souma Hiroki Hirayama Kenta Wada |
| author_facet | Yuichi Koshiishi Ryo Takahashi Michiko Murata-Okubo Yuichi Kameyama Kousaku Souma Hiroki Hirayama Kenta Wada |
| author_sort | Yuichi Koshiishi |
| collection | DOAJ |
| description | The emu (Dromaius novaehollandiae) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin 1-encoding gene (PLIN1) polymorphisms in the emus. We determined the partial complementary DNA (cDNA) sequence of the PLIN1, which is involved in lipid droplet formation. We identified four nucleotide substitution sites (c.270C>T, c.321T>C, c.587A>T, and c.639C>T) in the PLIN1 gene of emus. Of these, c.587A>T is a non-synonymous substitution that converts lysine to methionine at the 196th codon (p.K196M). Although p.K196M was predicted to affect the production traits of emus, a large deflection in genotype frequency was observed in this study; thus, we could not investigate the relationship between genotypes and phenotypes. In males, the fat yields of the CC, CT, and TT genotypes in c.270C>T were 0.25 ± 0.06, 0.22 ± 0.06, and 0.21 ± 0.07 kg, respectively, while the meat yields of the CC, CT, and TT genotypes in c.270C>T were 0.15 ± 0.01, 0.16 ± 0.02, and 0.16 ± 0.03 kg, respectively. These results indicate that male emus with the CC genotype had a significantly higher fat content and lower meat productivity than male emus with the other genotypes (P < 0.05). Therefore, c.270C>T in PLIN1 affects fat and meat production in males. Our findings may contribute to the effective genetic improvement of the emus. |
| format | Article |
| id | doaj-art-e24ffca9a7e249c4bed149025abe9a0d |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-e24ffca9a7e249c4bed149025abe9a0d2025-08-20T02:39:31ZengElsevierPoultry Science0032-57912024-12-011031210451310.1016/j.psj.2024.104513A PLIN1 polymorphism is associated with fat production in male emusYuichi Koshiishi0Ryo Takahashi1Michiko Murata-Okubo2Yuichi Kameyama3Kousaku Souma4Hiroki Hirayama5Kenta Wada6International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido 001-0020, JapanGraduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanGraduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanGraduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanGraduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanGraduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, JapanGraduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, Japan; Corresponding author: Abashiri, Hokkaido 099-2493, Japan.The emu (Dromaius novaehollandiae) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin 1-encoding gene (PLIN1) polymorphisms in the emus. We determined the partial complementary DNA (cDNA) sequence of the PLIN1, which is involved in lipid droplet formation. We identified four nucleotide substitution sites (c.270C>T, c.321T>C, c.587A>T, and c.639C>T) in the PLIN1 gene of emus. Of these, c.587A>T is a non-synonymous substitution that converts lysine to methionine at the 196th codon (p.K196M). Although p.K196M was predicted to affect the production traits of emus, a large deflection in genotype frequency was observed in this study; thus, we could not investigate the relationship between genotypes and phenotypes. In males, the fat yields of the CC, CT, and TT genotypes in c.270C>T were 0.25 ± 0.06, 0.22 ± 0.06, and 0.21 ± 0.07 kg, respectively, while the meat yields of the CC, CT, and TT genotypes in c.270C>T were 0.15 ± 0.01, 0.16 ± 0.02, and 0.16 ± 0.03 kg, respectively. These results indicate that male emus with the CC genotype had a significantly higher fat content and lower meat productivity than male emus with the other genotypes (P < 0.05). Therefore, c.270C>T in PLIN1 affects fat and meat production in males. Our findings may contribute to the effective genetic improvement of the emus.http://www.sciencedirect.com/science/article/pii/S0032579124010915EmuPerilipinPLIN1 polymorphisms |
| spellingShingle | Yuichi Koshiishi Ryo Takahashi Michiko Murata-Okubo Yuichi Kameyama Kousaku Souma Hiroki Hirayama Kenta Wada A PLIN1 polymorphism is associated with fat production in male emus Poultry Science Emu Perilipin PLIN1 polymorphisms |
| title | A PLIN1 polymorphism is associated with fat production in male emus |
| title_full | A PLIN1 polymorphism is associated with fat production in male emus |
| title_fullStr | A PLIN1 polymorphism is associated with fat production in male emus |
| title_full_unstemmed | A PLIN1 polymorphism is associated with fat production in male emus |
| title_short | A PLIN1 polymorphism is associated with fat production in male emus |
| title_sort | plin1 polymorphism is associated with fat production in male emus |
| topic | Emu Perilipin PLIN1 polymorphisms |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124010915 |
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