Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province
Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR) technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample collected of lesion exudates patients. Methodology: The (115) sample were examined by...
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| Format: | Article |
| Language: | English |
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University of Kufa, Faculty of Nursing
2013-08-01
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| Series: | Kufa Journal for Nursing Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2461 |
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| author | Haitham Mohammed Alawadi Ban Shakir Alshukur |
| author_facet | Haitham Mohammed Alawadi Ban Shakir Alshukur |
| author_sort | Haitham Mohammed Alawadi |
| collection | DOAJ |
| description |
Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR) technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample collected of lesion exudates patients.
Methodology: The (115) sample were examined by smear slid preparation culture on RPMI 1640 and NNN then DNA isolation. The DNA of the promastigote were amplified by PCR including primers selected on repetitive KDNA for identification of leishmania species. The data was analyzed by using frequency and percentage.
Results: The PCR result showed that two species of leishmania (L. major with 620 bp and L. tropica with 800 bp) exist.
Conclusion: It is concluded of the present study that the PCR technique has high specificity and sensitivity during the differentiation between Cutaneous Leishmanial species in comparing with conventional methods.
Recommendation: The PCR technique can be use it to diagnosis and differentiation between different type of Cutaneous Leishmaniasis.
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| format | Article |
| id | doaj-art-e24dbb09d6bc493981b6b65f9433819b |
| institution | OA Journals |
| issn | 2223-4055 2521-8638 |
| language | English |
| publishDate | 2013-08-01 |
| publisher | University of Kufa, Faculty of Nursing |
| record_format | Article |
| series | Kufa Journal for Nursing Sciences |
| spelling | doaj-art-e24dbb09d6bc493981b6b65f9433819b2025-08-20T02:11:53ZengUniversity of Kufa, Faculty of NursingKufa Journal for Nursing Sciences2223-40552521-86382013-08-013210.36321/kjns.vi20132.2461Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf ProvinceHaitham Mohammed Alawadi0Ban Shakir Alshukur1University of KufaUniversity of Kufa Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR) technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample collected of lesion exudates patients. Methodology: The (115) sample were examined by smear slid preparation culture on RPMI 1640 and NNN then DNA isolation. The DNA of the promastigote were amplified by PCR including primers selected on repetitive KDNA for identification of leishmania species. The data was analyzed by using frequency and percentage. Results: The PCR result showed that two species of leishmania (L. major with 620 bp and L. tropica with 800 bp) exist. Conclusion: It is concluded of the present study that the PCR technique has high specificity and sensitivity during the differentiation between Cutaneous Leishmanial species in comparing with conventional methods. Recommendation: The PCR technique can be use it to diagnosis and differentiation between different type of Cutaneous Leishmaniasis. https://journal.uokufa.edu.iq/index.php/kjns/article/view/2461Identification diagnosisCutaneous Leishmaniasispolymerasechain |
| spellingShingle | Haitham Mohammed Alawadi Ban Shakir Alshukur Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province Kufa Journal for Nursing Sciences Identification diagnosis Cutaneous Leishmaniasis polymerase chain |
| title | Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province |
| title_full | Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province |
| title_fullStr | Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province |
| title_full_unstemmed | Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province |
| title_short | Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province |
| title_sort | identification and diagnosis of cutaneous leishmaninsis by polymerase chain reaction assay pcr in najaf alashraf province |
| topic | Identification diagnosis Cutaneous Leishmaniasis polymerase chain |
| url | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2461 |
| work_keys_str_mv | AT haithammohammedalawadi identificationanddiagnosisofcutaneousleishmaninsisbypolymerasechainreactionassaypcrinnajafalashrafprovince AT banshakiralshukur identificationanddiagnosisofcutaneousleishmaninsisbypolymerasechainreactionassaypcrinnajafalashrafprovince |