Integrating Nanopore MinION Sequencing into National Animal Health AMR Surveillance Programs: An Indonesian Pilot Study of Chicken Slaughterhouse Effluent and Rivers

Integrating Nanopore MinION Sequencing into National Animal Health AMR Surveillance Programs: An Indonesian Pilot Study of Chicken Slaughterhouse Effluent and Rivers

<b>Background:</b> Antimicrobial resistance (AMR) poses significant risks to human and animal health, while the environment can contribute to its spread. National AMR surveillance programs are pivotal for assessing AMR prevalence, trends, and intervention outcomes; however, integrating a...

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Main Authors: Rallya Telussa, Puji Rahayu, Thufeil Yunindika, Curtis J. Kapsak, Kanti Puji Rahayu, Oli Susanti, Imron Suandy, Nuraini Triwijayanti, Aji B. Niasono, Syamsul Ma’arif, Hendra Wibawa, Lestari Lestari, Gunawan B. Utomo, Farida C. Zenal, Luuk Schoonman, Lee E. Voth-Gaeddert
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Antibiotics
Subjects:
<i>Escherichia coli</i>
waterborne pathogens
extended-spectrum beta-lactamases (ESBLs)
genomic sequencing
Tricycle Protocol
Online Access:https://www.mdpi.com/2079-6382/14/7/624
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Summary:<b>Background:</b> Antimicrobial resistance (AMR) poses significant risks to human and animal health, while the environment can contribute to its spread. National AMR surveillance programs are pivotal for assessing AMR prevalence, trends, and intervention outcomes; however, integrating advanced surveillance tools can be difficult. This pilot study, conducted by FAO ECTAD Indonesia and DGLAHS, the Indonesian Ministry of Agriculture, evaluated the costs and benefits of integrating the Nanopore MinION, Illumina MiSeq, and Sensititre system into a culture-based slaughterhouse–river surveillance system. <b>Methods:</b> Water samples were collected from six chicken slaughterhouses and adjacent rivers (pre- and post-treatment effluent, upstream, and downstream). Culture-based ESBL and general <i>E. coli</i> concentrations were estimated via the WHO Tricycle Protocol, while isolates (<i>n</i> = 42) were sequenced (MinION, MiSeq) and antimicrobial susceptibility testing conducted (Sensititre). <b>Results:</b> The Tricycle Protocol results provided estimates of effluent and river concentrations of ESBL and general <i>E. coli</i> identifying ESBL-to-general <i>E. coli</i> ratios of 13.8% and 6.2%, respectively. Compared to hybrid sequencing assemblies, MinION had a higher concordance than MiSeq for ARG identification (98%), virulence genes (96%), and locations for both (predominately plasmids). Furthermore, MinION concordance with Sensititre AST was 91%. <b>Conclusions:</b> Cost–benefit comparisons suggest sequencing can complement culture-based methods but is dependent on the value placed on the additional information gained.
ISSN:2079-6382

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