A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight
Fire blight, a devastating bacterial disease caused by <i>Erwinia amylovora</i>, has posed significant challenges to apple and pear production for over a century. This study introduces a gliding filamentous bacterium, the strain NSD29, isolated from natural forest soil in Xinjiang, China...
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2025-05-01
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| author | Wen Lv Ruiyue Wang Wenbo Ji Benzhong Fu Ming Luo Jian Han |
| author_facet | Wen Lv Ruiyue Wang Wenbo Ji Benzhong Fu Ming Luo Jian Han |
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| description | Fire blight, a devastating bacterial disease caused by <i>Erwinia amylovora</i>, has posed significant challenges to apple and pear production for over a century. This study introduces a gliding filamentous bacterium, the strain NSD29, isolated from natural forest soil in Xinjiang, China, as a biological control agent for managing this disease. Comprehensive characterization based on morphological, physiological, biochemical, 16S rRNA gene, and whole-genome analyses identified the strain NSD29 as <i>Herpetosiphon llansteffanensis</i>. The train NSD29 demonstrated potent predatory activity against <i>E. amylovora</i> in vitro. Its biocontrol efficacy was subsequently evaluated on detached leaves, inflorescences, young fruit, and shoots of fragrant pear under controlled greenhouse conditions. Results indicated that applying <i>H</i>. <i>llansteffanensis</i> NSD29 significantly inhibited lesion expansion on pear leaves and young fruit, achieving protective efficacies of 75.2% and 72.0%, respectively. Furthermore, pre-treatment spraying with NSD29 effectively reduced the incidence of blossom blight, with a control efficacy of 61.2%. On detached pear shoots, the application of NSD29 fermentation broth suppressed lesion expansion, demonstrating substantial protective (86.8%) and curative (75.6%) efficacies. This research provides the first evidence for the potential of <i>Herpetosiphon</i> species in the biological control of plant diseases, highlighting <i>H. llansteffanensis</i> NSD29 as a promising candidate for developing strategies to combat fire blight. |
| format | Article |
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| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-e2488c42fc394d7ab0ea2e2614ddb84c2025-08-20T03:11:32ZengMDPI AGPlants2223-77472025-05-011411156410.3390/plants14111564A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire BlightWen Lv0Ruiyue Wang1Wenbo Ji2Benzhong Fu3Ming Luo4Jian Han5Department of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaDepartment of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaDepartment of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaDepartment of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaDepartment of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaDepartment of Plant Pathology, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaFire blight, a devastating bacterial disease caused by <i>Erwinia amylovora</i>, has posed significant challenges to apple and pear production for over a century. This study introduces a gliding filamentous bacterium, the strain NSD29, isolated from natural forest soil in Xinjiang, China, as a biological control agent for managing this disease. Comprehensive characterization based on morphological, physiological, biochemical, 16S rRNA gene, and whole-genome analyses identified the strain NSD29 as <i>Herpetosiphon llansteffanensis</i>. The train NSD29 demonstrated potent predatory activity against <i>E. amylovora</i> in vitro. Its biocontrol efficacy was subsequently evaluated on detached leaves, inflorescences, young fruit, and shoots of fragrant pear under controlled greenhouse conditions. Results indicated that applying <i>H</i>. <i>llansteffanensis</i> NSD29 significantly inhibited lesion expansion on pear leaves and young fruit, achieving protective efficacies of 75.2% and 72.0%, respectively. Furthermore, pre-treatment spraying with NSD29 effectively reduced the incidence of blossom blight, with a control efficacy of 61.2%. On detached pear shoots, the application of NSD29 fermentation broth suppressed lesion expansion, demonstrating substantial protective (86.8%) and curative (75.6%) efficacies. This research provides the first evidence for the potential of <i>Herpetosiphon</i> species in the biological control of plant diseases, highlighting <i>H. llansteffanensis</i> NSD29 as a promising candidate for developing strategies to combat fire blight.https://www.mdpi.com/2223-7747/14/11/1564predatory<i>Erwinia amylovora</i>biological controlantibacterial activity |
| spellingShingle | Wen Lv Ruiyue Wang Wenbo Ji Benzhong Fu Ming Luo Jian Han A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight Plants predatory <i>Erwinia amylovora</i> biological control antibacterial activity |
| title | A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight |
| title_full | A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight |
| title_fullStr | A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight |
| title_full_unstemmed | A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight |
| title_short | A <i>Herpetosiphon llansteffanensis</i> Strain from Forest Soil Exhibits Biocontrol Activity Against Pear Fire Blight |
| title_sort | i herpetosiphon llansteffanensis i strain from forest soil exhibits biocontrol activity against pear fire blight |
| topic | predatory <i>Erwinia amylovora</i> biological control antibacterial activity |
| url | https://www.mdpi.com/2223-7747/14/11/1564 |
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