An updated reference genome of Barbatula barbatula (Linnaeus, 1758)
Abstract The stone loach Barbatula barbatula is a benthic fish species widely distributed throughout Europe, primarily inhabiting stony upper sections of stream networks. This study presents an updated genome assembly of B. barbatula, contributing to the species’ available genomic resources for down...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04469-z |
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| author | Levente Laczkó Nikoletta Andrea Nagy Ágnes Nagy Ágnes Maroda Péter Sály |
| author_facet | Levente Laczkó Nikoletta Andrea Nagy Ágnes Nagy Ágnes Maroda Péter Sály |
| author_sort | Levente Laczkó |
| collection | DOAJ |
| description | Abstract The stone loach Barbatula barbatula is a benthic fish species widely distributed throughout Europe, primarily inhabiting stony upper sections of stream networks. This study presents an updated genome assembly of B. barbatula, contributing to the species’ available genomic resources for downstream applications such as conservation genetics. The draft assembly was 550 Mbp in size, with an N50 of 11.21 Mbp. We used the species’ available chromosome scaffolds to finish the genome. The final assembly had a BUSCO score of 96.7%. We identified 23270 protein-coding genes, and the proteome exhibited high completeness with BUSCO (93.1%) and OMArk (90.81%). Despite using multiple approaches to reduce duplicate contigs, we observed a relatively high duplicate ratio of 6.1% (BUSCO) and 8.52% (OMArk) in the annotations. We aimed to find microsatellite loci present in both the species’ publicly available genome and the new assembly to aid marker development for downstream analyses. This dataset serves as a reference for genomic analysis and is useful for developing markers to study the species’ biodiversity and support conservation efforts. |
| format | Article |
| id | doaj-art-e23da3eab3784426ab8f74b7ca49c71b |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-e23da3eab3784426ab8f74b7ca49c71b2025-01-26T12:14:54ZengNature PortfolioScientific Data2052-44632025-01-011211910.1038/s41597-025-04469-zAn updated reference genome of Barbatula barbatula (Linnaeus, 1758)Levente Laczkó0Nikoletta Andrea Nagy1Ágnes Nagy2Ágnes Maroda3Péter Sály4One Health Institute, University of DebrecenInstitute of Metagenomics, University of DebrecenHungarian Defence Forces Medical CentreMATE Department of Zoology and Ecology, Hungarian University of Agriculture and Life SciencesHUN-REN Institite of Aquatic Ecology, Centre for Ecological ResearchAbstract The stone loach Barbatula barbatula is a benthic fish species widely distributed throughout Europe, primarily inhabiting stony upper sections of stream networks. This study presents an updated genome assembly of B. barbatula, contributing to the species’ available genomic resources for downstream applications such as conservation genetics. The draft assembly was 550 Mbp in size, with an N50 of 11.21 Mbp. We used the species’ available chromosome scaffolds to finish the genome. The final assembly had a BUSCO score of 96.7%. We identified 23270 protein-coding genes, and the proteome exhibited high completeness with BUSCO (93.1%) and OMArk (90.81%). Despite using multiple approaches to reduce duplicate contigs, we observed a relatively high duplicate ratio of 6.1% (BUSCO) and 8.52% (OMArk) in the annotations. We aimed to find microsatellite loci present in both the species’ publicly available genome and the new assembly to aid marker development for downstream analyses. This dataset serves as a reference for genomic analysis and is useful for developing markers to study the species’ biodiversity and support conservation efforts.https://doi.org/10.1038/s41597-025-04469-z |
| spellingShingle | Levente Laczkó Nikoletta Andrea Nagy Ágnes Nagy Ágnes Maroda Péter Sály An updated reference genome of Barbatula barbatula (Linnaeus, 1758) Scientific Data |
| title | An updated reference genome of Barbatula barbatula (Linnaeus, 1758) |
| title_full | An updated reference genome of Barbatula barbatula (Linnaeus, 1758) |
| title_fullStr | An updated reference genome of Barbatula barbatula (Linnaeus, 1758) |
| title_full_unstemmed | An updated reference genome of Barbatula barbatula (Linnaeus, 1758) |
| title_short | An updated reference genome of Barbatula barbatula (Linnaeus, 1758) |
| title_sort | updated reference genome of barbatula barbatula linnaeus 1758 |
| url | https://doi.org/10.1038/s41597-025-04469-z |
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