Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing
Aims: Rotator cuff tear (RCT) is the leading cause of shoulder pain, primarily associated with age-related tendon degeneration. This study aimed to elucidate the potential differential gene expressions in tendons across different age groups, and to investigate their roles in tendon degeneration. Me...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The British Editorial Society of Bone & Joint Surgery
2024-09-01
|
| Series: | Bone & Joint Research |
| Subjects: | |
| Online Access: | https://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.139.BJR-2023-0398.R1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850285369755435008 |
|---|---|
| author | Yibin Liu Xing Li Lei Jiang Jinjin Ma |
| author_facet | Yibin Liu Xing Li Lei Jiang Jinjin Ma |
| author_sort | Yibin Liu |
| collection | DOAJ |
| description | Aims: Rotator cuff tear (RCT) is the leading cause of shoulder pain, primarily associated with age-related tendon degeneration. This study aimed to elucidate the potential differential gene expressions in tendons across different age groups, and to investigate their roles in tendon degeneration. Methods: Linear regression and differential expression (DE) analyses were performed on two transcriptome profiling datasets of torn supraspinatus tendons to identify age-related genes. Subsequent functional analyses were conducted on these candidate genes to explore their potential roles in tendon ageing. Additionally, a secondary DE analysis was performed on candidate genes by comparing their expressions between lesioned and normal tendons to explore their correlations with RCTs. Results: We identified 49 genes in torn supraspinatus tendons associated with advancing age. Among them, five age-related genes showed DE in lesioned tendons compared to normal tendons. Functional analyses and previous studies have highlighted their specific enrichments in biological functions, such as muscle development (e.g. myosin heavy chain 3 (MYH3)), transcription regulation (e.g. CCAAT enhancer binding brotein delta (CEBPD)), and metal ion homeostasis (e.g. metallothionein 1X (MT1X)). Conclusion: This study uncovered molecular aspects of tendon ageing and their potential links to RCT development, offering insights for targeted interventions. These findings enhance our understanding of the mechanisms of tendon degeneration, allowing potential strategies to be made for reducing the incidence of RCT. Cite this article: Bone Joint Res 2024;13(9):474–484. |
| format | Article |
| id | doaj-art-e23ad32a120242bfa56e6d5662b0f5a2 |
| institution | OA Journals |
| issn | 2046-3758 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | The British Editorial Society of Bone & Joint Surgery |
| record_format | Article |
| series | Bone & Joint Research |
| spelling | doaj-art-e23ad32a120242bfa56e6d5662b0f5a22025-08-20T01:47:18ZengThe British Editorial Society of Bone & Joint SurgeryBone & Joint Research2046-37582024-09-0113947448410.1302/2046-3758.139.BJR-2023-0398.R1Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageingYibin Liu0Xing Li1Lei Jiang2Jinjin Ma3https://orcid.org/0000-0002-5446-2214 School of Medicine, South China University of Technology, Guangzhou, China School of Medicine, South China University of Technology, Guangzhou, China Department of Cardiology, Guangdong Provincial People's Hospital, Guangzhou, Guangdong, China School of Medicine, South China University of Technology, Guangzhou, China Aims: Rotator cuff tear (RCT) is the leading cause of shoulder pain, primarily associated with age-related tendon degeneration. This study aimed to elucidate the potential differential gene expressions in tendons across different age groups, and to investigate their roles in tendon degeneration. Methods: Linear regression and differential expression (DE) analyses were performed on two transcriptome profiling datasets of torn supraspinatus tendons to identify age-related genes. Subsequent functional analyses were conducted on these candidate genes to explore their potential roles in tendon ageing. Additionally, a secondary DE analysis was performed on candidate genes by comparing their expressions between lesioned and normal tendons to explore their correlations with RCTs. Results: We identified 49 genes in torn supraspinatus tendons associated with advancing age. Among them, five age-related genes showed DE in lesioned tendons compared to normal tendons. Functional analyses and previous studies have highlighted their specific enrichments in biological functions, such as muscle development (e.g. myosin heavy chain 3 (MYH3)), transcription regulation (e.g. CCAAT enhancer binding brotein delta (CEBPD)), and metal ion homeostasis (e.g. metallothionein 1X (MT1X)). Conclusion: This study uncovered molecular aspects of tendon ageing and their potential links to RCT development, offering insights for targeted interventions. These findings enhance our understanding of the mechanisms of tendon degeneration, allowing potential strategies to be made for reducing the incidence of RCT. Cite this article: Bone Joint Res 2024;13(9):474–484.https://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.139.BJR-2023-0398.R1rotator cuff teartendonageinggene expressionsrotator cuff tendonstendonsgene expressionslesionedrctstendon degenerationsupraspinatus tendonsmetal ionshoulder paint-tests |
| spellingShingle | Yibin Liu Xing Li Lei Jiang Jinjin Ma Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing Bone & Joint Research rotator cuff tear tendon ageing gene expressions rotator cuff tendons tendons gene expressions lesioned rcts tendon degeneration supraspinatus tendons metal ion shoulder pain t-tests |
| title | Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing |
| title_full | Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing |
| title_fullStr | Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing |
| title_full_unstemmed | Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing |
| title_short | Identification of age-related genes in rotator cuff tendon: a preliminary transcriptome study on tendon ageing |
| title_sort | identification of age related genes in rotator cuff tendon a preliminary transcriptome study on tendon ageing |
| topic | rotator cuff tear tendon ageing gene expressions rotator cuff tendons tendons gene expressions lesioned rcts tendon degeneration supraspinatus tendons metal ion shoulder pain t-tests |
| url | https://online.boneandjoint.org.uk/doi/epdf/10.1302/2046-3758.139.BJR-2023-0398.R1 |
| work_keys_str_mv | AT yibinliu identificationofagerelatedgenesinrotatorcufftendonapreliminarytranscriptomestudyontendonageing AT xingli identificationofagerelatedgenesinrotatorcufftendonapreliminarytranscriptomestudyontendonageing AT leijiang identificationofagerelatedgenesinrotatorcufftendonapreliminarytranscriptomestudyontendonageing AT jinjinma identificationofagerelatedgenesinrotatorcufftendonapreliminarytranscriptomestudyontendonageing |