Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics

Pseudomonas aeruginosa, an important Gram-negative opportunistic pathogen, is accountable for numerous acute and chronic infections, resulting in mortality rates of up to 40 % and significant morbidity. This opportunistic bacterial pathogen targets individuals with conditions like cystic fibrosis, b...

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Main Authors: Nomita Gupta, Kanchan Chauhan, Garima Singh, Shobhi Chaudhary, Jitendra Singh Rathore
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:The Microbe
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950194625000019
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author Nomita Gupta
Kanchan Chauhan
Garima Singh
Shobhi Chaudhary
Jitendra Singh Rathore
author_facet Nomita Gupta
Kanchan Chauhan
Garima Singh
Shobhi Chaudhary
Jitendra Singh Rathore
author_sort Nomita Gupta
collection DOAJ
description Pseudomonas aeruginosa, an important Gram-negative opportunistic pathogen, is accountable for numerous acute and chronic infections, resulting in mortality rates of up to 40 % and significant morbidity. This opportunistic bacterial pathogen targets individuals with conditions like cystic fibrosis, burn wounds, chronic obstructive pulmonary disorder (COPD), immunodeficiency, cancer, and those experiencing severe infections requiring ventilation, such as in the case of COVID-19. Treatment with antibiotics is currently the only means of preventing P. aeruginosa infections. The pathogens acquired and adaptive mechanisms have led to a rise in the prevalence of multidrug-resistance, which poses a significant challenge in healthcare environments. The bacterial pathogen uses many strategies such as the formation of biofilms, multidrug resistance, and antibiotic tolerance, to spread and maintain infection. The mechanisms of intrinsic and adaptive resistance utilized by this microbe to empower its pathogenesis are covered in great detail in this review. The different antibiotics that are employed in clinical settings to treat P. aeruginosa infections are also discussed. Furthermore, various therapeutic strategies to treat P. aeruginosa infections such as phage therapy and CRISPR-Cas9, antimicrobial peptides, immunomodulators, use of nanotechnology, vaccine development, and the revolutionary concept of drug repurposing are well discussed. Coherently, we draw attention to the research on alternative as well as innovative methods to control P. aeruginosa infections replacing the need of traditionally available antibiotics.
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spelling doaj-art-e237a064939c4657a8b4e90659d7778c2025-08-20T03:42:55ZengElsevierThe Microbe2950-19462025-03-01610023310.1016/j.microb.2025.100233Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibioticsNomita Gupta0Kanchan Chauhan1Garima Singh2Shobhi Chaudhary3Jitendra Singh Rathore4Gautam Buddha University, School of Biotechnology, Yamuna Expressway, Greater Noida, Uttar Pradesh 201312, IndiaGautam Buddha University, School of Biotechnology, Yamuna Expressway, Greater Noida, Uttar Pradesh 201312, IndiaGautam Buddha University, School of Biotechnology, Yamuna Expressway, Greater Noida, Uttar Pradesh 201312, IndiaGautam Buddha University, School of Biotechnology, Yamuna Expressway, Greater Noida, Uttar Pradesh 201312, IndiaCorrespondence to: School of Biotechnology, Gautam Buddha University, Yamuna Expressway, Gautam Buddha Nagar, Greater Noida, Uttar Pradesh 201312, India.; Gautam Buddha University, School of Biotechnology, Yamuna Expressway, Greater Noida, Uttar Pradesh 201312, IndiaPseudomonas aeruginosa, an important Gram-negative opportunistic pathogen, is accountable for numerous acute and chronic infections, resulting in mortality rates of up to 40 % and significant morbidity. This opportunistic bacterial pathogen targets individuals with conditions like cystic fibrosis, burn wounds, chronic obstructive pulmonary disorder (COPD), immunodeficiency, cancer, and those experiencing severe infections requiring ventilation, such as in the case of COVID-19. Treatment with antibiotics is currently the only means of preventing P. aeruginosa infections. The pathogens acquired and adaptive mechanisms have led to a rise in the prevalence of multidrug-resistance, which poses a significant challenge in healthcare environments. The bacterial pathogen uses many strategies such as the formation of biofilms, multidrug resistance, and antibiotic tolerance, to spread and maintain infection. The mechanisms of intrinsic and adaptive resistance utilized by this microbe to empower its pathogenesis are covered in great detail in this review. The different antibiotics that are employed in clinical settings to treat P. aeruginosa infections are also discussed. Furthermore, various therapeutic strategies to treat P. aeruginosa infections such as phage therapy and CRISPR-Cas9, antimicrobial peptides, immunomodulators, use of nanotechnology, vaccine development, and the revolutionary concept of drug repurposing are well discussed. Coherently, we draw attention to the research on alternative as well as innovative methods to control P. aeruginosa infections replacing the need of traditionally available antibiotics.http://www.sciencedirect.com/science/article/pii/S2950194625000019P. aeruginosaAntibioticsResistanceTherapeuticsAntibiotic-free strategies
spellingShingle Nomita Gupta
Kanchan Chauhan
Garima Singh
Shobhi Chaudhary
Jitendra Singh Rathore
Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics
The Microbe
P. aeruginosa
Antibiotics
Resistance
Therapeutics
Antibiotic-free strategies
title Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics
title_full Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics
title_fullStr Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics
title_full_unstemmed Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics
title_short Decoding antibiotic resistance in Pseudomonas aeruginosa: Embracing innovative therapies beyond conventional antibiotics
title_sort decoding antibiotic resistance in pseudomonas aeruginosa embracing innovative therapies beyond conventional antibiotics
topic P. aeruginosa
Antibiotics
Resistance
Therapeutics
Antibiotic-free strategies
url http://www.sciencedirect.com/science/article/pii/S2950194625000019
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