RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
Survival of mesenchymal stem cells in the bone marrow is essential for bone marrow microenvironment homeostasis, but the molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule of programmed cell death in tissue homeostasis. However, little is known about the regulati...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5540149 |
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| _version_ | 1850167165136666624 |
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| author | Qing Tian Chen Cao Weijian Qiu Han Wu Lijun Zhou Zhipeng Dai Zhenwei Li Songfeng Chen |
| author_facet | Qing Tian Chen Cao Weijian Qiu Han Wu Lijun Zhou Zhipeng Dai Zhenwei Li Songfeng Chen |
| author_sort | Qing Tian |
| collection | DOAJ |
| description | Survival of mesenchymal stem cells in the bone marrow is essential for bone marrow microenvironment homeostasis, but the molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule of programmed cell death in tissue homeostasis. However, little is known about the regulation of RIPK1 on bone marrow mesenchymal stem cells (MSCs). Here, we have investigated for the first time the role of RIPK1 in bone marrow MSCs. We have found that RIPK1 knockdown suppressed proliferation, differentiation, and migration in bone marrow MSCs. Furthermore, RIPK1 knockdown resulted in the opening of mitochondrial permeability transition pore (mPTP) and mtDNA damage, leading to mitochondrial dysfunction, and consequently induced apoptosis and necroptosis in bone marrow MSCs. Moreover, we identified that the p53-PUMA axis pathway was involved in mitochondrial dysfunction in RIPK1-deficient bone marrow MSCs. Together, our findings highlighted that RIPK1 was indispensable for bone marrow MSC survival. |
| format | Article |
| id | doaj-art-e235d15c963140de90940c29135daf1f |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e235d15c963140de90940c29135daf1f2025-08-20T02:21:16ZengWileyStem Cells International1687-96782021-01-01202110.1155/2021/5540149RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53Qing Tian0Chen Cao1Weijian Qiu2Han Wu3Lijun Zhou4Zhipeng Dai5Zhenwei Li6Songfeng Chen7Department of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsDepartment of OrthopaedicsSurvival of mesenchymal stem cells in the bone marrow is essential for bone marrow microenvironment homeostasis, but the molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule of programmed cell death in tissue homeostasis. However, little is known about the regulation of RIPK1 on bone marrow mesenchymal stem cells (MSCs). Here, we have investigated for the first time the role of RIPK1 in bone marrow MSCs. We have found that RIPK1 knockdown suppressed proliferation, differentiation, and migration in bone marrow MSCs. Furthermore, RIPK1 knockdown resulted in the opening of mitochondrial permeability transition pore (mPTP) and mtDNA damage, leading to mitochondrial dysfunction, and consequently induced apoptosis and necroptosis in bone marrow MSCs. Moreover, we identified that the p53-PUMA axis pathway was involved in mitochondrial dysfunction in RIPK1-deficient bone marrow MSCs. Together, our findings highlighted that RIPK1 was indispensable for bone marrow MSC survival.http://dx.doi.org/10.1155/2021/5540149 |
| spellingShingle | Qing Tian Chen Cao Weijian Qiu Han Wu Lijun Zhou Zhipeng Dai Zhenwei Li Songfeng Chen RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53 Stem Cells International |
| title | RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53 |
| title_full | RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53 |
| title_fullStr | RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53 |
| title_full_unstemmed | RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53 |
| title_short | RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53 |
| title_sort | ripk1 coordinates bone marrow mesenchymal stem cell survival by maintaining mitochondrial homeostasis via p53 |
| url | http://dx.doi.org/10.1155/2021/5540149 |
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