Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment
Objective(s): In this study, to improve anti-melanogenesis properties, the green method based on an ultrasonic approach was utilized to prepare lavender essential oil (LEO) nanoemulgel.Material and Methods: Nano-emulsion green preparation, physico-chemical characterization, inspection morphology, an...
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Mashhad University of Medical Sciences
2025-07-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_25574_3e43b43404761cc0680c45f711979309.pdf |
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| author | Mahmoud Omidi Katayoun Morteza-Semnani Majid Saeedi Mina Frahmand Marzie Kamali Esmaeil Mohammadian Seyyed Mohammad Hassan Hashemi |
| author_facet | Mahmoud Omidi Katayoun Morteza-Semnani Majid Saeedi Mina Frahmand Marzie Kamali Esmaeil Mohammadian Seyyed Mohammad Hassan Hashemi |
| author_sort | Mahmoud Omidi |
| collection | DOAJ |
| description | Objective(s): In this study, to improve anti-melanogenesis properties, the green method based on an ultrasonic approach was utilized to prepare lavender essential oil (LEO) nanoemulgel.Material and Methods: Nano-emulsion green preparation, physico-chemical characterization, inspection morphology, animal safety study, cellar toxicity and anti-pigmentation test were performed.Results: The obtained results showed that with a decline in HLB value (Hydrophilic–lipophilic balance), the mean particle size declined from 157.600±3.798 to 85.566± 2.227 nm (P< 0.05). The evaluations performed with the accelerated stability test (freeze-thaw cycle) showed stability for nanoemulgel. Other factors, such as pH, spreadability, and, viscosity were also measured. In vitro cytotoxicity studies for the revealed LEO-nanoemulsion, on the HFF normal cell line showed less toxicity (73%) than pure LEO. Moreover, LEO-nanoemulsion had a higher cytotoxic impact on B16F10 melanoma cancer cells than pure LEO. The inhibitory activity of this LEO-nanoemulsion was evaluated in melanin content, which indicated that melanin synthesis is more inhibited than LEO. Furthermore, the evaluation of the inhibitory activity presented that the inhibition of L-dopa auto oxidation (87.33± 2.45%) is better than that of LEO (74.22± 3.26%) at a concentration of 5000 µg/ml. It should be noted that no skin irritation was observed with the histo-pathological examination on Wistar rat and the dermal irritation study for the LEO-nanoemulgel.Conclusion: The results of the present study can be very useful for the introduction and local administration of LEO nanoemulgel for the management of hyperpigmentation conditions. |
| format | Article |
| id | doaj-art-e235438832524ee2a483816678eb8c20 |
| institution | DOAJ |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Mashhad University of Medical Sciences |
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| series | Nanomedicine Journal |
| spelling | doaj-art-e235438832524ee2a483816678eb8c202025-08-20T02:46:04ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-07-0112340442010.22038/nmj.2025.80954.200425574Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessmentMahmoud Omidi0Katayoun Morteza-Semnani1Majid Saeedi2Mina Frahmand3Marzie Kamali4Esmaeil Mohammadian5Seyyed Mohammad Hassan Hashemi6Food Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, IranDepartment of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, IranStudent Research Committee, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, IranMolecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, IranFood Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, IranFood Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, IranObjective(s): In this study, to improve anti-melanogenesis properties, the green method based on an ultrasonic approach was utilized to prepare lavender essential oil (LEO) nanoemulgel.Material and Methods: Nano-emulsion green preparation, physico-chemical characterization, inspection morphology, animal safety study, cellar toxicity and anti-pigmentation test were performed.Results: The obtained results showed that with a decline in HLB value (Hydrophilic–lipophilic balance), the mean particle size declined from 157.600±3.798 to 85.566± 2.227 nm (P< 0.05). The evaluations performed with the accelerated stability test (freeze-thaw cycle) showed stability for nanoemulgel. Other factors, such as pH, spreadability, and, viscosity were also measured. In vitro cytotoxicity studies for the revealed LEO-nanoemulsion, on the HFF normal cell line showed less toxicity (73%) than pure LEO. Moreover, LEO-nanoemulsion had a higher cytotoxic impact on B16F10 melanoma cancer cells than pure LEO. The inhibitory activity of this LEO-nanoemulsion was evaluated in melanin content, which indicated that melanin synthesis is more inhibited than LEO. Furthermore, the evaluation of the inhibitory activity presented that the inhibition of L-dopa auto oxidation (87.33± 2.45%) is better than that of LEO (74.22± 3.26%) at a concentration of 5000 µg/ml. It should be noted that no skin irritation was observed with the histo-pathological examination on Wistar rat and the dermal irritation study for the LEO-nanoemulgel.Conclusion: The results of the present study can be very useful for the introduction and local administration of LEO nanoemulgel for the management of hyperpigmentation conditions.https://nmj.mums.ac.ir/article_25574_3e43b43404761cc0680c45f711979309.pdflavenderessential oilnanotechnologygreen methodanti-melanogenesis |
| spellingShingle | Mahmoud Omidi Katayoun Morteza-Semnani Majid Saeedi Mina Frahmand Marzie Kamali Esmaeil Mohammadian Seyyed Mohammad Hassan Hashemi Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment Nanomedicine Journal lavender essential oil nanotechnology green method anti-melanogenesis |
| title | Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment |
| title_full | Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment |
| title_fullStr | Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment |
| title_full_unstemmed | Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment |
| title_short | Lavender essential oil nanoemulsion gel as skin lightener: green formulation, full characterization, anti-melanogenesis effect, and in-vitro/in-vivo safety profile assessment |
| title_sort | lavender essential oil nanoemulsion gel as skin lightener green formulation full characterization anti melanogenesis effect and in vitro in vivo safety profile assessment |
| topic | lavender essential oil nanotechnology green method anti-melanogenesis |
| url | https://nmj.mums.ac.ir/article_25574_3e43b43404761cc0680c45f711979309.pdf |
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