Impact of CYP3A4 functional variability on ziprasidone metabolism

IntroductionZiprasidone is primarily metabolized by CYP3A4, an enzyme with genetic variability and susceptibility to inhibition or induction. This study explored the functional variability of CYP3A4 in ziprasidone metabolism, focusing on drug interactions and genetic polymorphisms.MethodsThe metabol...

Full description

Saved in:
Bibliographic Details
Main Authors: Qi Zhou, Yameng Wu, Zhize Ye, Zheyan Zhang, Kai Zheng, Jianchang Qian, Zhongxiang Xiao, Yang Lu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Pharmacology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1585040/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850176085065465856
author Qi Zhou
Qi Zhou
Yameng Wu
Zhize Ye
Zheyan Zhang
Kai Zheng
Jianchang Qian
Zhongxiang Xiao
Yang Lu
author_facet Qi Zhou
Qi Zhou
Yameng Wu
Zhize Ye
Zheyan Zhang
Kai Zheng
Jianchang Qian
Zhongxiang Xiao
Yang Lu
author_sort Qi Zhou
collection DOAJ
description IntroductionZiprasidone is primarily metabolized by CYP3A4, an enzyme with genetic variability and susceptibility to inhibition or induction. This study explored the functional variability of CYP3A4 in ziprasidone metabolism, focusing on drug interactions and genetic polymorphisms.MethodsThe metabolic inhibition and kinetic properties of ziprasidone were evaluated through in vitro experiments utilizing rat liver microsomes (RLM), human liver microsomes (HLM), and CYP3A4 baculosomes. In vivo validation studies were conducted in Sprague-Dawley rats.ResultsQuercetin significantly inhibited ziprasidone metabolism in vitro, with in vivo coadministration led to marked increasing in ziprasidone’s AUC, CLz/F, and Cmax. Inhibition followed mixed mechanisms in RLM, HLM, and CYP3A4.1 systems. Analysis of CYP3A4 variants revealed distinct metabolic efficiencies: CYP3A4.3, 15, and 33 exhibited elevated clearance, while CYP3A4.24, 31, and 34 showed reduced activity. Quercetin’s inhibitory potency varied across alleles, with IC50 values of 17.59 ± 1.01 μM in CYP3A4.1 and 54.51 ± 1.35 μM in CYP3A4.33. Molecular docking identified ARG106, PHE108, PHE215, THR224, and GLU374 as key residues mediating inhibition.DiscussionThe findings of this study underscore the critical role of quercetin-mediated CYP3A4 inhibition and CYP3A4 genetic polymorphisms in modulating ziprasidone metabolism.
format Article
id doaj-art-e2333299a2c340c7bd085b9fcd45053b
institution OA Journals
issn 1663-9812
language English
publishDate 2025-04-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Pharmacology
spelling doaj-art-e2333299a2c340c7bd085b9fcd45053b2025-08-20T02:19:19ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-04-011610.3389/fphar.2025.15850401585040Impact of CYP3A4 functional variability on ziprasidone metabolismQi Zhou0Qi Zhou1Yameng Wu2Zhize Ye3Zheyan Zhang4Kai Zheng5Jianchang Qian6Zhongxiang Xiao7Yang Lu8Affiliated Yueqing Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaSchool of Pharmaceutical Sciences, Institute of Molecular Toxicology and Pharmacology, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaAffiliated Yueqing Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaDepartment of Pharmacy, Shaoxing People’s Hospital, Shaoxing, ChinaSchool of Pharmaceutical Sciences, Institute of Molecular Toxicology and Pharmacology, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaAffiliated Yueqing Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaSchool of Pharmaceutical Sciences, Institute of Molecular Toxicology and Pharmacology, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaAffiliated Yueqing Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaAffiliated Yueqing Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaIntroductionZiprasidone is primarily metabolized by CYP3A4, an enzyme with genetic variability and susceptibility to inhibition or induction. This study explored the functional variability of CYP3A4 in ziprasidone metabolism, focusing on drug interactions and genetic polymorphisms.MethodsThe metabolic inhibition and kinetic properties of ziprasidone were evaluated through in vitro experiments utilizing rat liver microsomes (RLM), human liver microsomes (HLM), and CYP3A4 baculosomes. In vivo validation studies were conducted in Sprague-Dawley rats.ResultsQuercetin significantly inhibited ziprasidone metabolism in vitro, with in vivo coadministration led to marked increasing in ziprasidone’s AUC, CLz/F, and Cmax. Inhibition followed mixed mechanisms in RLM, HLM, and CYP3A4.1 systems. Analysis of CYP3A4 variants revealed distinct metabolic efficiencies: CYP3A4.3, 15, and 33 exhibited elevated clearance, while CYP3A4.24, 31, and 34 showed reduced activity. Quercetin’s inhibitory potency varied across alleles, with IC50 values of 17.59 ± 1.01 μM in CYP3A4.1 and 54.51 ± 1.35 μM in CYP3A4.33. Molecular docking identified ARG106, PHE108, PHE215, THR224, and GLU374 as key residues mediating inhibition.DiscussionThe findings of this study underscore the critical role of quercetin-mediated CYP3A4 inhibition and CYP3A4 genetic polymorphisms in modulating ziprasidone metabolism.https://www.frontiersin.org/articles/10.3389/fphar.2025.1585040/fullziprasidonequercetindrug-drug interactionCYP3A4LC-MS/MS
spellingShingle Qi Zhou
Qi Zhou
Yameng Wu
Zhize Ye
Zheyan Zhang
Kai Zheng
Jianchang Qian
Zhongxiang Xiao
Yang Lu
Impact of CYP3A4 functional variability on ziprasidone metabolism
Frontiers in Pharmacology
ziprasidone
quercetin
drug-drug interaction
CYP3A4
LC-MS/MS
title Impact of CYP3A4 functional variability on ziprasidone metabolism
title_full Impact of CYP3A4 functional variability on ziprasidone metabolism
title_fullStr Impact of CYP3A4 functional variability on ziprasidone metabolism
title_full_unstemmed Impact of CYP3A4 functional variability on ziprasidone metabolism
title_short Impact of CYP3A4 functional variability on ziprasidone metabolism
title_sort impact of cyp3a4 functional variability on ziprasidone metabolism
topic ziprasidone
quercetin
drug-drug interaction
CYP3A4
LC-MS/MS
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1585040/full
work_keys_str_mv AT qizhou impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT qizhou impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT yamengwu impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT zhizeye impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT zheyanzhang impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT kaizheng impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT jianchangqian impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT zhongxiangxiao impactofcyp3a4functionalvariabilityonziprasidonemetabolism
AT yanglu impactofcyp3a4functionalvariabilityonziprasidonemetabolism