Expression of VISTA on T cells in patients with cancer colon
Abstract Colorectal cancer (CRC) is one of the most common tumors in the world. A recent area of study for the treatment of patients with solid tumors is anti-tumor immunity. PD-1/PD-L1 inhibitors were beneficial for cancer patients with multiple tumor types. However, their efficacy for CRC is low....
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-05-01
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| Series: | Journal of the Egyptian National Cancer Institute |
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| Online Access: | https://doi.org/10.1186/s43046-025-00287-x |
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| author | Amira Gamal El Dein Badary Rania Bakry Khalid Rezk Maged AF Amine Asmaa M.Zahran |
| author_facet | Amira Gamal El Dein Badary Rania Bakry Khalid Rezk Maged AF Amine Asmaa M.Zahran |
| author_sort | Amira Gamal El Dein Badary |
| collection | DOAJ |
| description | Abstract Colorectal cancer (CRC) is one of the most common tumors in the world. A recent area of study for the treatment of patients with solid tumors is anti-tumor immunity. PD-1/PD-L1 inhibitors were beneficial for cancer patients with multiple tumor types. However, their efficacy for CRC is low. Thus, there is an urgent need to explore additional co-inhibitory tools such as VISTA for CRC treatment. Objective The current study aimed to evaluate expression of VISTA on T cell subsets in patients with CRC and its correlation with other prognostic markers. Patients and methods The study included 31 patients with CRC and 25 healthy controls. All participants were subjected to full history taking, clinical examination, routine laboratory investigations, and flow cytometric detection of VISTA expression on T cell subsets on peripheral blood (PB). In addition to detection of VISTA expression on T cell subsets on tissue samples of both malignant CRC and normal colon tissue of the CRC patients. Results In the peripheral blood, the expression of VISTA on CD4+ T helper and CD8+ T cytotoxic cells was significantly higher in CRC patients than the normal controls. There was no significant difference in VISTA expression on double positive T cells (CD4+CD8+) between the CRC patients and normal controls. In tissue samples, expression of VISTA on CD4 + T helper, CD8 + T cytotoxic, and double positive T (CD4 + CD8 +) cells in the malignant tissue of CRC patients was significantly higher than that in normal colonic tissue. Also, in CRC patients, the expression of VISTA on CD4+ T helper, CD8+ T cytotoxic, and double positive T cells in both malignant CRC tissue and normal colonic tissue was significantly higher than its expression PB. Conclusion The higher expression of VISTA in CRC patients than the healthy controls and its higher levels in malignant CRC tissue and normal colonic tissue than PB of CRC patients suggest the role VISTA in the pathogenesis of CRC. |
| format | Article |
| id | doaj-art-e22e15bac05f49d0a337919ff10dba5d |
| institution | DOAJ |
| issn | 2589-0409 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of the Egyptian National Cancer Institute |
| spelling | doaj-art-e22e15bac05f49d0a337919ff10dba5d2025-08-20T03:08:43ZengSpringerOpenJournal of the Egyptian National Cancer Institute2589-04092025-05-0137111910.1186/s43046-025-00287-xExpression of VISTA on T cells in patients with cancer colonAmira Gamal El Dein Badary0Rania Bakry1Khalid Rezk2Maged AF Amine3Asmaa M.Zahran4Molecular Biology Research & Studies InstituteMolecular Biology Research & Studies InstituteSouth Egypt Cancer Institute, Surgical Oncology DepartmentSouth Egypt Cancer Institute, Medical Oncology and Hematological malignanciesMolecular Biology Research & Studies InstituteAbstract Colorectal cancer (CRC) is one of the most common tumors in the world. A recent area of study for the treatment of patients with solid tumors is anti-tumor immunity. PD-1/PD-L1 inhibitors were beneficial for cancer patients with multiple tumor types. However, their efficacy for CRC is low. Thus, there is an urgent need to explore additional co-inhibitory tools such as VISTA for CRC treatment. Objective The current study aimed to evaluate expression of VISTA on T cell subsets in patients with CRC and its correlation with other prognostic markers. Patients and methods The study included 31 patients with CRC and 25 healthy controls. All participants were subjected to full history taking, clinical examination, routine laboratory investigations, and flow cytometric detection of VISTA expression on T cell subsets on peripheral blood (PB). In addition to detection of VISTA expression on T cell subsets on tissue samples of both malignant CRC and normal colon tissue of the CRC patients. Results In the peripheral blood, the expression of VISTA on CD4+ T helper and CD8+ T cytotoxic cells was significantly higher in CRC patients than the normal controls. There was no significant difference in VISTA expression on double positive T cells (CD4+CD8+) between the CRC patients and normal controls. In tissue samples, expression of VISTA on CD4 + T helper, CD8 + T cytotoxic, and double positive T (CD4 + CD8 +) cells in the malignant tissue of CRC patients was significantly higher than that in normal colonic tissue. Also, in CRC patients, the expression of VISTA on CD4+ T helper, CD8+ T cytotoxic, and double positive T cells in both malignant CRC tissue and normal colonic tissue was significantly higher than its expression PB. Conclusion The higher expression of VISTA in CRC patients than the healthy controls and its higher levels in malignant CRC tissue and normal colonic tissue than PB of CRC patients suggest the role VISTA in the pathogenesis of CRC.https://doi.org/10.1186/s43046-025-00287-xVISTAT cell subsetsColorectal cancer (CRC) |
| spellingShingle | Amira Gamal El Dein Badary Rania Bakry Khalid Rezk Maged AF Amine Asmaa M.Zahran Expression of VISTA on T cells in patients with cancer colon Journal of the Egyptian National Cancer Institute VISTA T cell subsets Colorectal cancer (CRC) |
| title | Expression of VISTA on T cells in patients with cancer colon |
| title_full | Expression of VISTA on T cells in patients with cancer colon |
| title_fullStr | Expression of VISTA on T cells in patients with cancer colon |
| title_full_unstemmed | Expression of VISTA on T cells in patients with cancer colon |
| title_short | Expression of VISTA on T cells in patients with cancer colon |
| title_sort | expression of vista on t cells in patients with cancer colon |
| topic | VISTA T cell subsets Colorectal cancer (CRC) |
| url | https://doi.org/10.1186/s43046-025-00287-x |
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