Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs
Abstract Background Although the importance of early diagnosis of malignant tumors is a major concern, an easily accessible in‐house method has not been established. Objectives To investigate the most optimal model by combining thymidine kinase 1 (TK1) autoantibody, extracellular protein kinase A (E...
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Wiley
2025-01-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.17266 |
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| author | Yoonhee Kim Wonhee Lee Byunggak Kim Bonghwan Jang Kyung‐Rok Yu Hong Sook Kim Minok Ryu Yein Oh Kyoungwon Seo Chul Park Ulsoo Choi Dongha Bhang Hwayoung Youn |
| author_facet | Yoonhee Kim Wonhee Lee Byunggak Kim Bonghwan Jang Kyung‐Rok Yu Hong Sook Kim Minok Ryu Yein Oh Kyoungwon Seo Chul Park Ulsoo Choi Dongha Bhang Hwayoung Youn |
| author_sort | Yoonhee Kim |
| collection | DOAJ |
| description | Abstract Background Although the importance of early diagnosis of malignant tumors is a major concern, an easily accessible in‐house method has not been established. Objectives To investigate the most optimal model by combining thymidine kinase 1 (TK1) autoantibody, extracellular protein kinase A (ECPKA) autoantibody, TK1 antigen, C‐reactive protein (CRP), age, breed, and sex. Animals Serum samples from 1702 dogs were collected from local animal hospitals and referral animal medical centers in Korea. Methods TK1 autoantibody, ECPKA autoantibody, and CRP were measured using LFIA methods in serum samples of dog to design a new neoplastic index (NI) for early detection of malignant tumors in dogs. Results AUC of TK1 autoantibody model with TK1 antigen, CRP, age, and breed in multiple logistic regression analysis was 0.966 (TK1 autoantibody, P = .0005; TK1 antigen, P = .0003), and when the cutoff value was 0.417, the specificity was 87.1%, and sensitivity was 96.4%. Regression coefficients were 24.4, 20.5, 24.8, 10.6, respectively in TK1 autoantibody, TK1 antigen, CRP, and age, and the effect by breed (regression coefficient 2.1, 3.0) was the lowest. The same multiple logistic regression analysis on dogs with lymphoma, and AUC of TK1 autoantibody model was 0.981 (all P < .0001, TK1 antigen P = .09), when the cutoff value was 0.352, the specificity was 92.9%, and sensitivity was 93.7%. Conclusions and Clinical Importance The NI including TK1 autoantibody could be useful in the screening test for both lymphoma and other tumors. |
| format | Article |
| id | doaj-art-e225ce25005141a2946ea09901694f9d |
| institution | Kabale University |
| issn | 0891-6640 1939-1676 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj-art-e225ce25005141a2946ea09901694f9d2025-01-27T15:22:41ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-01-01391n/an/a10.1111/jvim.17266Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogsYoonhee Kim0Wonhee Lee1Byunggak Kim2Bonghwan Jang3Kyung‐Rok Yu4Hong Sook Kim5Minok Ryu6Yein Oh7Kyoungwon Seo8Chul Park9Ulsoo Choi10Dongha Bhang11Hwayoung Youn12Department of Veterinary Clinical Pathology, College of Veterinary Medicine Jeonbuk National University Iksan‐si Jeollabuk‐do Republic of KoreaBiattic Inc., #306, 16, Heungan‐daero 427beon‐gil, Dongan‐gu Anyang‐si Gyeonggi‐do Republic of KoreaBiattic Inc., #306, 16, Heungan‐daero 427beon‐gil, Dongan‐gu Anyang‐si Gyeonggi‐do Republic of KoreaGood‐Morning‐Pet Animal Hospital, Jeongjail‐ro 55, Bundang‐gu, Seongnam‐si, Gyeonggi‐do Seoul South KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National University Seoul South KoreaDepartment of Biological Sciences Sungkyunkwan University, 2066 Seobu‐ro, Jangan‐gu Suwon Gyeonggi‐do South KoreaSeoul National University College of Veterinary Medicine, 1 Gwanak‐ro, Gwanak‐gu Seoul South KoreaCollege of Veterinary Medicine, Kyungpook National University, 80 Daehak‐ro, Buk‐gu Daegu South KoreaSeoul National University College of Veterinary Medicine, 1 Gwanak‐ro, Gwanak‐gu Seoul South KoreaDepartment of Veterinary Clinical Pathology, College of Veterinary Medicine Jeonbuk National University Iksan‐si Jeollabuk‐do Republic of KoreaDepartment of Veterinary Clinical Pathology, College of Veterinary Medicine Jeonbuk National University Iksan‐si Jeollabuk‐do Republic of KoreaBiattic Inc., #306, 16, Heungan‐daero 427beon‐gil, Dongan‐gu Anyang‐si Gyeonggi‐do Republic of KoreaSeoul National University College of Veterinary Medicine, 1 Gwanak‐ro, Gwanak‐gu Seoul South KoreaAbstract Background Although the importance of early diagnosis of malignant tumors is a major concern, an easily accessible in‐house method has not been established. Objectives To investigate the most optimal model by combining thymidine kinase 1 (TK1) autoantibody, extracellular protein kinase A (ECPKA) autoantibody, TK1 antigen, C‐reactive protein (CRP), age, breed, and sex. Animals Serum samples from 1702 dogs were collected from local animal hospitals and referral animal medical centers in Korea. Methods TK1 autoantibody, ECPKA autoantibody, and CRP were measured using LFIA methods in serum samples of dog to design a new neoplastic index (NI) for early detection of malignant tumors in dogs. Results AUC of TK1 autoantibody model with TK1 antigen, CRP, age, and breed in multiple logistic regression analysis was 0.966 (TK1 autoantibody, P = .0005; TK1 antigen, P = .0003), and when the cutoff value was 0.417, the specificity was 87.1%, and sensitivity was 96.4%. Regression coefficients were 24.4, 20.5, 24.8, 10.6, respectively in TK1 autoantibody, TK1 antigen, CRP, and age, and the effect by breed (regression coefficient 2.1, 3.0) was the lowest. The same multiple logistic regression analysis on dogs with lymphoma, and AUC of TK1 autoantibody model was 0.981 (all P < .0001, TK1 antigen P = .09), when the cutoff value was 0.352, the specificity was 92.9%, and sensitivity was 93.7%. Conclusions and Clinical Importance The NI including TK1 autoantibody could be useful in the screening test for both lymphoma and other tumors.https://doi.org/10.1111/jvim.17266autoantibodiesC‐reactive proteinextracellular protein kinase Amalignant tumorneoplastic indexthymidine kinase 1 |
| spellingShingle | Yoonhee Kim Wonhee Lee Byunggak Kim Bonghwan Jang Kyung‐Rok Yu Hong Sook Kim Minok Ryu Yein Oh Kyoungwon Seo Chul Park Ulsoo Choi Dongha Bhang Hwayoung Youn Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs Journal of Veterinary Internal Medicine autoantibodies C‐reactive protein extracellular protein kinase A malignant tumor neoplastic index thymidine kinase 1 |
| title | Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs |
| title_full | Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs |
| title_fullStr | Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs |
| title_full_unstemmed | Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs |
| title_short | Use of thymidine kinase 1 autoantibody, thymidine kinase antigen, extracellular protein kinase A autoantibody, and C‐reactive protein for early detection of malignant tumors in dogs |
| title_sort | use of thymidine kinase 1 autoantibody thymidine kinase antigen extracellular protein kinase a autoantibody and c reactive protein for early detection of malignant tumors in dogs |
| topic | autoantibodies C‐reactive protein extracellular protein kinase A malignant tumor neoplastic index thymidine kinase 1 |
| url | https://doi.org/10.1111/jvim.17266 |
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