Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis
BackgroundLong non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of autoimmune diseases. Our previous research demonstrated that AGAP2-AS1 in keratinocytes is involved in the pathogenesis of psoriasis, but its effect on CD4+ T cell differentiation remains unclear.MethodsExosomes wer...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2025.1521470/full |
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| author | Ziqi Yuan Xue Zeng Xiwei Zhang Chenglai Xia Xuebiao Peng |
| author_facet | Ziqi Yuan Xue Zeng Xiwei Zhang Chenglai Xia Xuebiao Peng |
| author_sort | Ziqi Yuan |
| collection | DOAJ |
| description | BackgroundLong non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of autoimmune diseases. Our previous research demonstrated that AGAP2-AS1 in keratinocytes is involved in the pathogenesis of psoriasis, but its effect on CD4+ T cell differentiation remains unclear.MethodsExosomes were extracted from HaCaT cells using a reagent kit and verified by TEM (Transmission Electron Microscope), NTA (Nanoparticle Tracking Analysis), and Western Blot. We incubated exosomes with CD4+ T cells and detected the distribution of AGAP2-AS1 by fluorescence microscopy. Flow cytometry and ELISA were used to detect CD4+ T cell differentiation. In addition, the relationship between AGAP2-AS1/miR-424-5p/SGK1 and its effect on CD4+ T cell differentiation were confirmed by bioinformatics analysis, dual luciferase reporter gene experiments, quantitative real-time PCR, flow cytometry, and ELISA.ResultsWe found that exosomes derived from TNF-α-treated HaCaTs were able to deliver AGAP2-AS1 to CD4+ T cells, promoting Th1 and Th17 differentiation. In CD4+ T cells, AGAP2-AS1 promotes Th1 and Th17 differentiation via the miR-424-5p/SGK1 axis.ConclusionPsoriatic HaCaTs deliver AGAP2-AS1 to CD4+ T cells via exosomes, inducing Th1 and Th17 differentiation through the miR-424-5p/SGK1 axis, thereby promoting the progression of psoriasis. These findings provide novel insights into the pathogenesis of psoriasis and potential therapeutic targets. |
| format | Article |
| id | doaj-art-e21a2cc0b92c4636850220db8f9aad96 |
| institution | DOAJ |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-e21a2cc0b92c4636850220db8f9aad962025-08-20T03:21:34ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-05-011610.3389/fgene.2025.15214701521470Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasisZiqi Yuan0Xue Zeng1Xiwei Zhang2Chenglai Xia3Xuebiao Peng4Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaFoshan Maternity and Child Healthcare Hospital, Foshan, ChinaDepartment of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaBackgroundLong non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of autoimmune diseases. Our previous research demonstrated that AGAP2-AS1 in keratinocytes is involved in the pathogenesis of psoriasis, but its effect on CD4+ T cell differentiation remains unclear.MethodsExosomes were extracted from HaCaT cells using a reagent kit and verified by TEM (Transmission Electron Microscope), NTA (Nanoparticle Tracking Analysis), and Western Blot. We incubated exosomes with CD4+ T cells and detected the distribution of AGAP2-AS1 by fluorescence microscopy. Flow cytometry and ELISA were used to detect CD4+ T cell differentiation. In addition, the relationship between AGAP2-AS1/miR-424-5p/SGK1 and its effect on CD4+ T cell differentiation were confirmed by bioinformatics analysis, dual luciferase reporter gene experiments, quantitative real-time PCR, flow cytometry, and ELISA.ResultsWe found that exosomes derived from TNF-α-treated HaCaTs were able to deliver AGAP2-AS1 to CD4+ T cells, promoting Th1 and Th17 differentiation. In CD4+ T cells, AGAP2-AS1 promotes Th1 and Th17 differentiation via the miR-424-5p/SGK1 axis.ConclusionPsoriatic HaCaTs deliver AGAP2-AS1 to CD4+ T cells via exosomes, inducing Th1 and Th17 differentiation through the miR-424-5p/SGK1 axis, thereby promoting the progression of psoriasis. These findings provide novel insights into the pathogenesis of psoriasis and potential therapeutic targets.https://www.frontiersin.org/articles/10.3389/fgene.2025.1521470/fulllong non-coding RNAsmicrornaspsoriasist cellsexosomes |
| spellingShingle | Ziqi Yuan Xue Zeng Xiwei Zhang Chenglai Xia Xuebiao Peng Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis Frontiers in Genetics long non-coding RNAs micrornas psoriasis t cells exosomes |
| title | Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis |
| title_full | Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis |
| title_fullStr | Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis |
| title_full_unstemmed | Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis |
| title_short | Exosomes containing long non-coding RNA AGAP2-AS1 promote the differentiation of CD4+ T cells through the miR-424-5p/SGK1 axis in psoriasis |
| title_sort | exosomes containing long non coding rna agap2 as1 promote the differentiation of cd4 t cells through the mir 424 5p sgk1 axis in psoriasis |
| topic | long non-coding RNAs micrornas psoriasis t cells exosomes |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2025.1521470/full |
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