Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry
Acute poisoning has become the fifth leading cause of death among urban residents in China. A pivotal challenge in clinic is the rapid and precise identification of toxicants to guide targeted detoxification strategies. Conventional laboratory-based techniques, such as gas chromatography-mass spectr...
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Editorial Board of Journal of Chinese Mass Spectrometry Society
2025-07-01
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| Series: | Zhipu Xuebao |
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| Online Access: | https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.0207 |
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| author | Hui-wen RUAN Gui-yun SONG Zhi-gang FAN Suo-suo YANG Jian KANG Lei HUA Wei-guo WANG |
| author_facet | Hui-wen RUAN Gui-yun SONG Zhi-gang FAN Suo-suo YANG Jian KANG Lei HUA Wei-guo WANG |
| author_sort | Hui-wen RUAN |
| collection | DOAJ |
| description | Acute poisoning has become the fifth leading cause of death among urban residents in China. A pivotal challenge in clinic is the rapid and precise identification of toxicants to guide targeted detoxification strategies. Conventional laboratory-based techniques, such as gas chromatography-mass spectrometry (GC-MS), have the advantages of high sensitivity and high selectivity, but often require complex sample preparation, specialized operators, and prolonged turnaround times, rendering them unsuitable for rapid on-site detection. Miniaturized ion trap mass spectrometers have characteristics of high working pressure, tandem-in-time mass spectrometry functions, and field-portable design, which represent a promising approach for rapid on-site detection and hold significant potential for supporting the diagnosis and treatment of acute poisoning. In this study, a novel analytical platform integrating thermal desorption (TD) with a continuous atmospheric pressure interfaced ion trap mass spectrometry (CAPI-ITMS) was developed to rapidly and accurately detect common toxicants in blood matrices. The results demonstrated that this instrument exhibited excellent detection capabilities for three categories (nine types) of common toxicants, including pesticides, sedative hypnotics, and therapeutic drugs. To improve the accuracy of qualitative identification of toxicants in blood, the floating DC was adjusted to trigger collision-induced dissociation and obtain characteristic fragment ions of toxicants. In addition, combined with simple and efficient liquid-liquid extraction, the rapid and accurate detection of toxicants in blood was achieved within 2 min. The instrument exhibited good stability, with the relative standard deviation of 6.41% over 100 repeated tests. Based on a signal-to-noise ratio of 3, the detection limit of toxins in blood is in the range of 1.3-3.9 µg/L. The thermal desorption-continuous atmospheric pressure interfaced ion trap mass spectrometer not only provides critical guidance for targeted treatment of acute poisoning patients through precise qualitative analysis, but also enables the assessment of treatment effectiveness through quantitative analysis of the toxic substances. Given its cost-effectiveness and ease of operation, this instrument holds significant potential for widespread application in county-level hospitals with limited analytical resources, enabling rapid diagnosis of acute poisoning, real-time monitoring of drug levels, and disease detection. This approach provides doctors with precise and timely information to improve patient care quality and treatment efficiency. |
| format | Article |
| id | doaj-art-e21893476b4a498397a7868addb455b1 |
| institution | DOAJ |
| issn | 1004-2997 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Editorial Board of Journal of Chinese Mass Spectrometry Society |
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| series | Zhipu Xuebao |
| spelling | doaj-art-e21893476b4a498397a7868addb455b12025-08-20T03:15:32ZengEditorial Board of Journal of Chinese Mass Spectrometry SocietyZhipu Xuebao1004-29972025-07-0146441342110.7538/zpxb.2024.02072024-207-d15Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass SpectrometryHui-wen RUAN0Gui-yun SONG1Zhi-gang FAN2Suo-suo YANG3Jian KANG4Lei HUA5Wei-guo WANG6State Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaEmergency Department, the 1st Affiliated Hospital of Dalian Medical University, Dalian 116023, ChinaEmergency Department, the 1st Affiliated Hospital of Dalian Medical University, Dalian 116023, ChinaState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaState Key Laboratory of Medical Proteomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaAcute poisoning has become the fifth leading cause of death among urban residents in China. A pivotal challenge in clinic is the rapid and precise identification of toxicants to guide targeted detoxification strategies. Conventional laboratory-based techniques, such as gas chromatography-mass spectrometry (GC-MS), have the advantages of high sensitivity and high selectivity, but often require complex sample preparation, specialized operators, and prolonged turnaround times, rendering them unsuitable for rapid on-site detection. Miniaturized ion trap mass spectrometers have characteristics of high working pressure, tandem-in-time mass spectrometry functions, and field-portable design, which represent a promising approach for rapid on-site detection and hold significant potential for supporting the diagnosis and treatment of acute poisoning. In this study, a novel analytical platform integrating thermal desorption (TD) with a continuous atmospheric pressure interfaced ion trap mass spectrometry (CAPI-ITMS) was developed to rapidly and accurately detect common toxicants in blood matrices. The results demonstrated that this instrument exhibited excellent detection capabilities for three categories (nine types) of common toxicants, including pesticides, sedative hypnotics, and therapeutic drugs. To improve the accuracy of qualitative identification of toxicants in blood, the floating DC was adjusted to trigger collision-induced dissociation and obtain characteristic fragment ions of toxicants. In addition, combined with simple and efficient liquid-liquid extraction, the rapid and accurate detection of toxicants in blood was achieved within 2 min. The instrument exhibited good stability, with the relative standard deviation of 6.41% over 100 repeated tests. Based on a signal-to-noise ratio of 3, the detection limit of toxins in blood is in the range of 1.3-3.9 µg/L. The thermal desorption-continuous atmospheric pressure interfaced ion trap mass spectrometer not only provides critical guidance for targeted treatment of acute poisoning patients through precise qualitative analysis, but also enables the assessment of treatment effectiveness through quantitative analysis of the toxic substances. Given its cost-effectiveness and ease of operation, this instrument holds significant potential for widespread application in county-level hospitals with limited analytical resources, enabling rapid diagnosis of acute poisoning, real-time monitoring of drug levels, and disease detection. This approach provides doctors with precise and timely information to improve patient care quality and treatment efficiency.https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.0207photoionizationcontinuous atmospheric pressure interfaced ion trap mass spectrometryacute poisoningthermal desorptioncollision induced dissociation (cid) |
| spellingShingle | Hui-wen RUAN Gui-yun SONG Zhi-gang FAN Suo-suo YANG Jian KANG Lei HUA Wei-guo WANG Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry Zhipu Xuebao photoionization continuous atmospheric pressure interfaced ion trap mass spectrometry acute poisoning thermal desorption collision induced dissociation (cid) |
| title | Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry |
| title_full | Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry |
| title_fullStr | Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry |
| title_full_unstemmed | Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry |
| title_short | Rapid Detection of Trace Toxicants in Blood Using Thermal Desorption Coupled to Continuous Atmospheric Pressure Interfaced Ion Trap Mass Spectrometry |
| title_sort | rapid detection of trace toxicants in blood using thermal desorption coupled to continuous atmospheric pressure interfaced ion trap mass spectrometry |
| topic | photoionization continuous atmospheric pressure interfaced ion trap mass spectrometry acute poisoning thermal desorption collision induced dissociation (cid) |
| url | https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.0207 |
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