Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts
Glucosinolates are important phytochemicals in Brassicaceae. We investigated the effect of CaCl2-HCl electrolyzed water (CHEW) on glucosinolates biosynthesis in broccoli sprouts. The results showed that CHEW treatment significantly decreased reactive oxygen species (ROS) and malondialdeh yde (MDA) c...
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Tsinghua University Press
2024-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250068 |
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| author | Cui Li Shuhui Song Yanan He Haijie Liu |
| author_facet | Cui Li Shuhui Song Yanan He Haijie Liu |
| author_sort | Cui Li |
| collection | DOAJ |
| description | Glucosinolates are important phytochemicals in Brassicaceae. We investigated the effect of CaCl2-HCl electrolyzed water (CHEW) on glucosinolates biosynthesis in broccoli sprouts. The results showed that CHEW treatment significantly decreased reactive oxygen species (ROS) and malondialdeh yde (MDA) contents in broccoli sprouts. On the the 8th day, compared to tap water treatment, the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6% and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW. Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades. Besides, CHEW treatment not only promoted the biosynthesis of amino acids, but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors (MYBs, bHLHs, WRKYs, etc.). The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment. |
| format | Article |
| id | doaj-art-e2157f7852404e92aa5537445c05d2de |
| institution | DOAJ |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-e2157f7852404e92aa5537445c05d2de2025-08-20T03:20:59ZengTsinghua University PressFood Science and Human Wellness2213-45302024-03-0113280181210.26599/FSHW.2022.9250068Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sproutsCui Li0Shuhui Song1Yanan He2Haijie Liu3College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaInstitute of Agri-food Processing and Nutrition, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaGlucosinolates are important phytochemicals in Brassicaceae. We investigated the effect of CaCl2-HCl electrolyzed water (CHEW) on glucosinolates biosynthesis in broccoli sprouts. The results showed that CHEW treatment significantly decreased reactive oxygen species (ROS) and malondialdeh yde (MDA) contents in broccoli sprouts. On the the 8th day, compared to tap water treatment, the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6% and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW. Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades. Besides, CHEW treatment not only promoted the biosynthesis of amino acids, but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors (MYBs, bHLHs, WRKYs, etc.). The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.https://www.sciopen.com/article/10.26599/FSHW.2022.9250068broccoli sproutscacl2-hcl electrolyzed waterglucosinolatestranscriptomicsmetabolomics |
| spellingShingle | Cui Li Shuhui Song Yanan He Haijie Liu Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts Food Science and Human Wellness broccoli sprouts cacl2-hcl electrolyzed water glucosinolates transcriptomics metabolomics |
| title | Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts |
| title_full | Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts |
| title_fullStr | Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts |
| title_full_unstemmed | Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts |
| title_short | Transcriptomics integrated with metabolomics reveals the mechanism of CaCl2-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts |
| title_sort | transcriptomics integrated with metabolomics reveals the mechanism of cacl2 hcl electrolyzed water induced glucosinolate biosynthesis in broccoli sprouts |
| topic | broccoli sprouts cacl2-hcl electrolyzed water glucosinolates transcriptomics metabolomics |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250068 |
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