NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation
Abstract In an era where swift and precise diagnostic capabilities are paramount, we introduce NAPTUNE (Nucleic acids and Protein Biomarkers Testing via Ultra-sensitive Nucleases Escalation), an innovative platform for the amplification-free detection of nucleic acids and protein biomarkers in less...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56653-9 |
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| author | Tao Hu Xinxin Ke Yingying Yu Hongmei Feng Senfeng Zhang Yinuo Cui Boyang Zhang Min He Yinbing Tang Lei Liu Yu Lin Quanquan Ji Chuanxia Chen Chunlong Xu Chunyi Hu |
| author_facet | Tao Hu Xinxin Ke Yingying Yu Hongmei Feng Senfeng Zhang Yinuo Cui Boyang Zhang Min He Yinbing Tang Lei Liu Yu Lin Quanquan Ji Chuanxia Chen Chunlong Xu Chunyi Hu |
| author_sort | Tao Hu |
| collection | DOAJ |
| description | Abstract In an era where swift and precise diagnostic capabilities are paramount, we introduce NAPTUNE (Nucleic acids and Protein Biomarkers Testing via Ultra-sensitive Nucleases Escalation), an innovative platform for the amplification-free detection of nucleic acids and protein biomarkers in less than 45 minutes. Using a tandem cascade of endonucleases, NAPTUNE employs apurinic/apyrimidinic endonuclease 1 (APE1) to generate DNA guides, enabling the detection of target nucleic acids at femtomolar levels. The sensitivity is elevated to attomolar levels through the action of Pyrococcus furiosus Argonaute (PfAgo), which intensifies probe cleavage, thereby boosting both sensitivity and specificity within an innovative in-situ cascade circuit. This technology not only streamlines rapid, onsite diagnostics without pre-amplification but also demonstrates exceptional accuracy in identifying a broad spectrum of nucleic acids and crucial cancer-related protein biomarkers directly from clinical samples. The development of a portable device for point-of-care testing further underscores NAPTUNE’s potential to transform diagnostic processes, especially in resource-limited environments, marking a significant diversity forward in medical diagnostics and patient care. |
| format | Article |
| id | doaj-art-e20bae4bbad94ef8b4d1b5ce0d3b8284 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e20bae4bbad94ef8b4d1b5ce0d3b82842025-02-09T12:46:13ZengNature PortfolioNature Communications2041-17232025-02-0116111610.1038/s41467-025-56653-9NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalationTao Hu0Xinxin Ke1Yingying Yu2Hongmei Feng3Senfeng Zhang4Yinuo Cui5Boyang Zhang6Min He7Yinbing Tang8Lei Liu9Yu Lin10Quanquan Ji11Chuanxia Chen12Chunlong Xu13Chunyi Hu14Children’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineChildren’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineChildren’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineChildren’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineDepartment of Biological Sciences, Faculty of Science, National University of SingaporeDepartment of Biological Sciences, Faculty of Science, National University of SingaporeDepartment of Biological Sciences, Faculty of Science, National University of SingaporeDepartment of Surgical Oncology, Children’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineDepartment of Surgical Oncology, Children’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineDepartment of Pathology, Children’s Hospital, National Clinical Research Center for Child Health, Zhejiang University School of MedicineInternational Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Specialty, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong UniversityCancer Science Institute of Singapore, National University of SingaporeSchool of Materials Science and Engineering University of JinanLingang LaboratoryDepartment of Biological Sciences, Faculty of Science, National University of SingaporeAbstract In an era where swift and precise diagnostic capabilities are paramount, we introduce NAPTUNE (Nucleic acids and Protein Biomarkers Testing via Ultra-sensitive Nucleases Escalation), an innovative platform for the amplification-free detection of nucleic acids and protein biomarkers in less than 45 minutes. Using a tandem cascade of endonucleases, NAPTUNE employs apurinic/apyrimidinic endonuclease 1 (APE1) to generate DNA guides, enabling the detection of target nucleic acids at femtomolar levels. The sensitivity is elevated to attomolar levels through the action of Pyrococcus furiosus Argonaute (PfAgo), which intensifies probe cleavage, thereby boosting both sensitivity and specificity within an innovative in-situ cascade circuit. This technology not only streamlines rapid, onsite diagnostics without pre-amplification but also demonstrates exceptional accuracy in identifying a broad spectrum of nucleic acids and crucial cancer-related protein biomarkers directly from clinical samples. The development of a portable device for point-of-care testing further underscores NAPTUNE’s potential to transform diagnostic processes, especially in resource-limited environments, marking a significant diversity forward in medical diagnostics and patient care.https://doi.org/10.1038/s41467-025-56653-9 |
| spellingShingle | Tao Hu Xinxin Ke Yingying Yu Hongmei Feng Senfeng Zhang Yinuo Cui Boyang Zhang Min He Yinbing Tang Lei Liu Yu Lin Quanquan Ji Chuanxia Chen Chunlong Xu Chunyi Hu NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation Nature Communications |
| title | NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation |
| title_full | NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation |
| title_fullStr | NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation |
| title_full_unstemmed | NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation |
| title_short | NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation |
| title_sort | naptune nucleic acids and protein biomarkers testing via ultra sensitive nucleases escalation |
| url | https://doi.org/10.1038/s41467-025-56653-9 |
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