Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis.
RNA sequencing provides a new perspective on the genome of Mycobacterium tuberculosis by revealing an extensive presence of non-coding RNA, including long 5' and 3' untranslated regions, antisense transcripts, and intergenic small RNA (sRNA) molecules. More than a quarter of all sequence r...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-11-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002342&type=printable |
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| author | Kristine B Arnvig Iñaki Comas Nicholas R Thomson Joanna Houghton Helena I Boshoff Nicholas J Croucher Graham Rose Timothy T Perkins Julian Parkhill Gordon Dougan Douglas B Young |
| author_facet | Kristine B Arnvig Iñaki Comas Nicholas R Thomson Joanna Houghton Helena I Boshoff Nicholas J Croucher Graham Rose Timothy T Perkins Julian Parkhill Gordon Dougan Douglas B Young |
| author_sort | Kristine B Arnvig |
| collection | DOAJ |
| description | RNA sequencing provides a new perspective on the genome of Mycobacterium tuberculosis by revealing an extensive presence of non-coding RNA, including long 5' and 3' untranslated regions, antisense transcripts, and intergenic small RNA (sRNA) molecules. More than a quarter of all sequence reads mapping outside of ribosomal RNA genes represent non-coding RNA, and the density of reads mapping to intergenic regions was more than two-fold higher than that mapping to annotated coding sequences. Selected sRNAs were found at increased abundance in stationary phase cultures and accumulated to remarkably high levels in the lungs of chronically infected mice, indicating a potential contribution to pathogenesis. The ability of tubercle bacilli to adapt to changing environments within the host is critical to their ability to cause disease and to persist during drug treatment; it is likely that novel post-transcriptional regulatory networks will play an important role in these adaptive responses. |
| format | Article |
| id | doaj-art-e201ce0172db4061a864bdab7dba8543 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-e201ce0172db4061a864bdab7dba85432025-08-20T03:09:47ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742011-11-01711e100234210.1371/journal.ppat.1002342Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis.Kristine B ArnvigIñaki ComasNicholas R ThomsonJoanna HoughtonHelena I BoshoffNicholas J CroucherGraham RoseTimothy T PerkinsJulian ParkhillGordon DouganDouglas B YoungRNA sequencing provides a new perspective on the genome of Mycobacterium tuberculosis by revealing an extensive presence of non-coding RNA, including long 5' and 3' untranslated regions, antisense transcripts, and intergenic small RNA (sRNA) molecules. More than a quarter of all sequence reads mapping outside of ribosomal RNA genes represent non-coding RNA, and the density of reads mapping to intergenic regions was more than two-fold higher than that mapping to annotated coding sequences. Selected sRNAs were found at increased abundance in stationary phase cultures and accumulated to remarkably high levels in the lungs of chronically infected mice, indicating a potential contribution to pathogenesis. The ability of tubercle bacilli to adapt to changing environments within the host is critical to their ability to cause disease and to persist during drug treatment; it is likely that novel post-transcriptional regulatory networks will play an important role in these adaptive responses.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002342&type=printable |
| spellingShingle | Kristine B Arnvig Iñaki Comas Nicholas R Thomson Joanna Houghton Helena I Boshoff Nicholas J Croucher Graham Rose Timothy T Perkins Julian Parkhill Gordon Dougan Douglas B Young Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis. PLoS Pathogens |
| title | Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis. |
| title_full | Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis. |
| title_fullStr | Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis. |
| title_full_unstemmed | Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis. |
| title_short | Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis. |
| title_sort | sequence based analysis uncovers an abundance of non coding rna in the total transcriptome of mycobacterium tuberculosis |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1002342&type=printable |
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