Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran
Some evidence suggests that a variety of genetic factors contribute to development of the tuberculosis (TB). TLR4 and TLR9 have been proposed as susceptibility genes for TB. This study was performed in 124 newly diagnosed TB cases and 149 healthy controls in a TB-endemic region of Iran. The TLR4 gen...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/534053 |
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| author | Danial Jahantigh Saeedeh Salimi Roya Alavi-Naini Abolfazl Emamdadi Hamid Owaysee Osquee Farzaneh Farajian Mashhadi |
| author_facet | Danial Jahantigh Saeedeh Salimi Roya Alavi-Naini Abolfazl Emamdadi Hamid Owaysee Osquee Farzaneh Farajian Mashhadi |
| author_sort | Danial Jahantigh |
| collection | DOAJ |
| description | Some evidence suggests that a variety of genetic factors contribute to development of the tuberculosis (TB). TLR4 and TLR9 have been proposed as susceptibility genes for TB. This study was performed in 124 newly diagnosed TB cases and 149 healthy controls in a TB-endemic region of Iran. The TLR4 genes Asp299Gly, Thr399Ile, and TLR9 gene T-1486C polymorphisms were amplified by polymerase chain reaction (PCR) and then detected by PCR-restriction fragment length polymorphism (RFLP). The frequencies of the mutant alleles of TLR4 Arg299Gly, Thr399Ile, and TLR9 T-1486C polymorphisms were 0.8 versus 0.1, 5.6 versus 3, and 28.6 versus 25.2 in patients and controls, respectively, that were not significant. The synergic effect of TI,II/CC genotypes for TLR4 Thr399Ile and TLR9 T-1486C polymorphisms showed increased risk of PTB susceptibility. In conclusion, no significant relation was found between TLR4 and TLR9 polymorphisms alone and PTB. However, synergic effects of TLR4 Thr399Ile and TLR9-1486T/C polymorphisms might increase risk of PTB. |
| format | Article |
| id | doaj-art-e1f90b9efdac43f4ad1fb44e5fee3ea6 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e1f90b9efdac43f4ad1fb44e5fee3ea62025-02-03T07:26:11ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/534053534053Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern IranDanial Jahantigh0Saeedeh Salimi1Roya Alavi-Naini2Abolfazl Emamdadi3Hamid Owaysee Osquee4Farzaneh Farajian Mashhadi5Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IranCellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IranInfectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IranInfectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IranInfectious Diseases Department, Zabol University of Medical Sciences, Zabol, IranDepartment of Pharmacology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranSome evidence suggests that a variety of genetic factors contribute to development of the tuberculosis (TB). TLR4 and TLR9 have been proposed as susceptibility genes for TB. This study was performed in 124 newly diagnosed TB cases and 149 healthy controls in a TB-endemic region of Iran. The TLR4 genes Asp299Gly, Thr399Ile, and TLR9 gene T-1486C polymorphisms were amplified by polymerase chain reaction (PCR) and then detected by PCR-restriction fragment length polymorphism (RFLP). The frequencies of the mutant alleles of TLR4 Arg299Gly, Thr399Ile, and TLR9 T-1486C polymorphisms were 0.8 versus 0.1, 5.6 versus 3, and 28.6 versus 25.2 in patients and controls, respectively, that were not significant. The synergic effect of TI,II/CC genotypes for TLR4 Thr399Ile and TLR9 T-1486C polymorphisms showed increased risk of PTB susceptibility. In conclusion, no significant relation was found between TLR4 and TLR9 polymorphisms alone and PTB. However, synergic effects of TLR4 Thr399Ile and TLR9-1486T/C polymorphisms might increase risk of PTB.http://dx.doi.org/10.1155/2013/534053 |
| spellingShingle | Danial Jahantigh Saeedeh Salimi Roya Alavi-Naini Abolfazl Emamdadi Hamid Owaysee Osquee Farzaneh Farajian Mashhadi Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran The Scientific World Journal |
| title | Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran |
| title_full | Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran |
| title_fullStr | Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran |
| title_full_unstemmed | Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran |
| title_short | Association between TLR4 and TLR9 Gene Polymorphisms with Development of Pulmonary Tuberculosis in Zahedan, Southeastern Iran |
| title_sort | association between tlr4 and tlr9 gene polymorphisms with development of pulmonary tuberculosis in zahedan southeastern iran |
| url | http://dx.doi.org/10.1155/2013/534053 |
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