Exploring Core Genes Associated with Sepsis and Systemic Inflammatory Response Syndrome Using Single-Cell Sequencing Technology
YuZhou Shen,1 LingHan Leng,2 YingChun Hu1 1Department of Emergency Medicine, the Affiliated Hospital of Southwest Medical University, Lu Zhou, Sichuan, People’s Republic of China; 2Department of Intensive Care Unit, Chengdu Fifth People’s Hospital, Chengdu, Sichuan, People’s Republic of ChinaCorresp...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-02-01
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| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/exploring-core-genes-associated-with-sepsis-and-systemic-inflammatory--peer-reviewed-fulltext-article-JIR |
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| Summary: | YuZhou Shen,1 LingHan Leng,2 YingChun Hu1 1Department of Emergency Medicine, the Affiliated Hospital of Southwest Medical University, Lu Zhou, Sichuan, People’s Republic of China; 2Department of Intensive Care Unit, Chengdu Fifth People’s Hospital, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: YingChun Hu, Department of Emergency Medicine, The Affiliated Hospital of Southwest Medical University, 25 Taiping Street, Jiang Yang District, Lu Zhou, Sichuan, People’s Republic of China, Tel +86-15228232720, Fax +86-830-3165120, Email huyingchun913@swmu.edu.cnPurpose: As a crucial aspect of emergency critical medicine, sepsis has been in a difficult stage. As its ”preparatory stage”, SIRS has attracted the attention of the medical workers all over the world. The frequency of occurrence is on the rise, but there is a lack of certain indicators for the timely detection and recognition of illnesses.Methods: By virtue of scRNA-seq, this research has analyzed single-cell transcriptome data from samples taken from groups with septic death and systemic inflammatory response syndrome so as to identify the unique markers and patterns in immune response.Results: By revealing the status of twelve cell clusters of four major cell types in blood samples through UMAP cell clustering and the differences of major cell populations between the dead and SIRS patients, the results have elucidated the components of different cells and their marker genes in two disease states, and the response mechanism beneficial to disease diagnosis in blood samples.Conclusion: By establishing a theoretical framework centered on cellular and molecular regulation, the study has introduced a novel approach for diagnosing and treating sepsis death group and SIRS patients early, as well as differentiating and preventing these conditions.Keywords: sepsis, SIRS, single cell sequencing, biomarkers, single cell transcriptome |
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| ISSN: | 1178-7031 |