Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos
Abstract LVNC (Left Ventricular Non-Compaction), which is a type of cardiomyopathy characterized by an abnormal heart muscle structure. Retinoic acid (RA) is crucial for normal heart development, including the regulation of myocardial differentiation and chamber formation. Cyp26b1 plays a key role i...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05497-5 |
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| author | Zhao Zhang Xueting Chen Shuxin Su Silian Chen Li Pang Juan Shen Xiaodong Fang Zhe Zhang |
| author_facet | Zhao Zhang Xueting Chen Shuxin Su Silian Chen Li Pang Juan Shen Xiaodong Fang Zhe Zhang |
| author_sort | Zhao Zhang |
| collection | DOAJ |
| description | Abstract LVNC (Left Ventricular Non-Compaction), which is a type of cardiomyopathy characterized by an abnormal heart muscle structure. Retinoic acid (RA) is crucial for normal heart development, including the regulation of myocardial differentiation and chamber formation. Cyp26b1 plays a key role in regulating RA signaling by degrading RA. However, more targeted research is needed to directly link Cyp26b1 with LVNC pathology. To explore the effect of Cyp26b1 on heart development, we collected heart tissues from wild type (WT) and Cyp26b1 knockout (KO) mice at four time points (E10.5-E13.5) and performed single-cell RNA sequencing. We obtained 134,499 high-quality cells (57,923 WT and 62,488 KO) after filtering. The data quality was confirmed through various evaluation index and analytical methods mapping rates. Our initial analysis identified 10 major cell types in hearts, and differential expression analysis revealed the transcriptional change after deletion of Cyp26b1, particularly in cardiomyocytes. Collectively, these data offer a valuable resource for researchers investigating the role of Cyp26b1 in heart development. |
| format | Article |
| id | doaj-art-e1d4bf161d4b4487b6b5c9a9aa2c0fec |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-e1d4bf161d4b4487b6b5c9a9aa2c0fec2025-08-20T04:01:47ZengNature PortfolioScientific Data2052-44632025-07-011211910.1038/s41597-025-05497-5Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryosZhao Zhang0Xueting Chen1Shuxin Su2Silian Chen3Li Pang4Juan Shen5Xiaodong Fang6Zhe Zhang7College of Life Sciences, University of Chinese Academy of SciencesCollege of Life Sciences, University of Chinese Academy of SciencesCollege of Life Sciences, University of Chinese Academy of SciencesCollege of Life Sciences, University of Chinese Academy of SciencesBGI ResearchBGI ResearchBGI ResearchDepartment of Cardiology, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University)Abstract LVNC (Left Ventricular Non-Compaction), which is a type of cardiomyopathy characterized by an abnormal heart muscle structure. Retinoic acid (RA) is crucial for normal heart development, including the regulation of myocardial differentiation and chamber formation. Cyp26b1 plays a key role in regulating RA signaling by degrading RA. However, more targeted research is needed to directly link Cyp26b1 with LVNC pathology. To explore the effect of Cyp26b1 on heart development, we collected heart tissues from wild type (WT) and Cyp26b1 knockout (KO) mice at four time points (E10.5-E13.5) and performed single-cell RNA sequencing. We obtained 134,499 high-quality cells (57,923 WT and 62,488 KO) after filtering. The data quality was confirmed through various evaluation index and analytical methods mapping rates. Our initial analysis identified 10 major cell types in hearts, and differential expression analysis revealed the transcriptional change after deletion of Cyp26b1, particularly in cardiomyocytes. Collectively, these data offer a valuable resource for researchers investigating the role of Cyp26b1 in heart development.https://doi.org/10.1038/s41597-025-05497-5 |
| spellingShingle | Zhao Zhang Xueting Chen Shuxin Su Silian Chen Li Pang Juan Shen Xiaodong Fang Zhe Zhang Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos Scientific Data |
| title | Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos |
| title_full | Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos |
| title_fullStr | Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos |
| title_full_unstemmed | Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos |
| title_short | Single-cell RNA sequencing dataset of hearts from wild type and Cyp26b1 knockout mouse embryos |
| title_sort | single cell rna sequencing dataset of hearts from wild type and cyp26b1 knockout mouse embryos |
| url | https://doi.org/10.1038/s41597-025-05497-5 |
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