Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand
Toxoplasmosis is a significant parasitic zoonosis caused by <i>Toxoplasma gondii</i>. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. T...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/6/555 |
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| author | Pongpisid Koonyosying Anucha Muenthaisong Kanokwan Sangkakam Kanpitcha Nontasaya Amarin Rittipornlertrak Boondarika Nambooppha Nisachon Apinda Supawadee Maneekesorn Nattawooti Sthitmatee |
| author_facet | Pongpisid Koonyosying Anucha Muenthaisong Kanokwan Sangkakam Kanpitcha Nontasaya Amarin Rittipornlertrak Boondarika Nambooppha Nisachon Apinda Supawadee Maneekesorn Nattawooti Sthitmatee |
| author_sort | Pongpisid Koonyosying |
| collection | DOAJ |
| description | Toxoplasmosis is a significant parasitic zoonosis caused by <i>Toxoplasma gondii</i>. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of <i>T. gondii</i> infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the <i>T. gondii</i> dihydrofolate reductase-thymidylate synthase (<i>Tgdhfr-ts</i>) gene was employed for molecular detection. This represents the first report of <i>T. gondii</i> molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for <i>T. gondii</i> DNA. The selected DNA sequences from the positive <i>T. gondii</i> DNA displayed a high degree of nucleotide sequence homology with the reference <i>Tgdhfr-ts</i> sequence. Phylogenetic analysis revealed a single clade alongside other <i>T. gondii</i> strains, showing no differentiation based on genotype. This study contributes to the understanding of <i>T. gondii</i> epidemiology and provides a foundation for future strategies to control and manage <i>T. gondii</i> transmission in livestock populations. |
| format | Article |
| id | doaj-art-e1d255622ae1417bb3a6a4321d4ac308 |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-e1d255622ae1417bb3a6a4321d4ac3082025-08-20T03:32:31ZengMDPI AGVeterinary Sciences2306-73812025-06-0112655510.3390/vetsci12060555Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern ThailandPongpisid Koonyosying0Anucha Muenthaisong1Kanokwan Sangkakam2Kanpitcha Nontasaya3Amarin Rittipornlertrak4Boondarika Nambooppha5Nisachon Apinda6Supawadee Maneekesorn7Nattawooti Sthitmatee8Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandToxoplasmosis is a significant parasitic zoonosis caused by <i>Toxoplasma gondii</i>. Among livestock animals, small ruminants, especially sheep and goats, are the most infected. This infection is a leading cause of abortion, resulting in considerable economic losses for goat breeders. The present study aimed to assess the prevalence of <i>T. gondii</i> infection in goats in northern Thailand, with an emphasis on its potential zoonotic transmission to humans. Polymerase chain reaction (PCR) targeting the <i>T. gondii</i> dihydrofolate reductase-thymidylate synthase (<i>Tgdhfr-ts</i>) gene was employed for molecular detection. This represents the first report of <i>T. gondii</i> molecular detection in blood samples from small ruminants in Thailand by PCR. A total of 176 meat goat blood samples were analyzed, yielding an 8.52% (15/176) positivity rate for <i>T. gondii</i> DNA. The selected DNA sequences from the positive <i>T. gondii</i> DNA displayed a high degree of nucleotide sequence homology with the reference <i>Tgdhfr-ts</i> sequence. Phylogenetic analysis revealed a single clade alongside other <i>T. gondii</i> strains, showing no differentiation based on genotype. This study contributes to the understanding of <i>T. gondii</i> epidemiology and provides a foundation for future strategies to control and manage <i>T. gondii</i> transmission in livestock populations.https://www.mdpi.com/2306-7381/12/6/555<i>Toxoplasma gondii</i>goat toxoplasmosisdihydrofolate reductase thymidylate synthase (<i>dhfr-ts</i>)molecular detectionpolymerase chain reaction (PCR)goat blood samples |
| spellingShingle | Pongpisid Koonyosying Anucha Muenthaisong Kanokwan Sangkakam Kanpitcha Nontasaya Amarin Rittipornlertrak Boondarika Nambooppha Nisachon Apinda Supawadee Maneekesorn Nattawooti Sthitmatee Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand Veterinary Sciences <i>Toxoplasma gondii</i> goat toxoplasmosis dihydrofolate reductase thymidylate synthase (<i>dhfr-ts</i>) molecular detection polymerase chain reaction (PCR) goat blood samples |
| title | Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand |
| title_full | Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand |
| title_fullStr | Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand |
| title_full_unstemmed | Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand |
| title_short | Molecular Detection of Genetic Material of <i>Toxoplasma gondii</i> in Goat Blood Samples from Northern Thailand |
| title_sort | molecular detection of genetic material of i toxoplasma gondii i in goat blood samples from northern thailand |
| topic | <i>Toxoplasma gondii</i> goat toxoplasmosis dihydrofolate reductase thymidylate synthase (<i>dhfr-ts</i>) molecular detection polymerase chain reaction (PCR) goat blood samples |
| url | https://www.mdpi.com/2306-7381/12/6/555 |
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