Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent
A spectrophotometric method was developed for the determination of methyldopa. The method is based on oxidative coupling reactions with 4,2-dinitrophenylhydrazine as a reagent and in the presence of the oxidizing agent potassium periodate in the acidic medium. The product exhibits maximum absorptio...
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| Language: | English |
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Tikrit University
2022-11-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/10 |
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| author | Dhuha N. Ali AL-ghanam Mohammed S. Sheet AL-Enizzi |
| author_facet | Dhuha N. Ali AL-ghanam Mohammed S. Sheet AL-Enizzi |
| author_sort | Dhuha N. Ali AL-ghanam |
| collection | DOAJ |
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A spectrophotometric method was developed for the determination of methyldopa. The method is based on oxidative coupling reactions with 4,2-dinitrophenylhydrazine as a reagent and in the presence of the oxidizing agent potassium periodate in the acidic medium. The product exhibits maximum absorption at 428 nm, Beer's law-subjective concentration in the range of (1-30) µg/ml, and molar absorbance of 6589,908 liters. mol-1.cm-1. The relative standard deviation was 0.418 percent, the recovery rate was 99.60 percent, and the quantitative limit attained (LOQ) was 0.261 µg/ml. The limit of detection (LOD) was 0.078µg/ml. Following the procedures of continuous changes and molar ratio, the nature of the product created from the reaction was examined, and the ratio was 2:1. (drug compound: reagent) and the value of the stable rate of stability was 8.84 x 1012 liters 2. mol-2, which indicates the good stability of this product. This method was successfully applied to estimate methyldopa in tablet form
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| format | Article |
| id | doaj-art-e1c7ad84a70f45a49e2df776c015572e |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-e1c7ad84a70f45a49e2df776c015572e2025-08-20T03:49:56ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262022-11-0127510.25130/tjps.v27i5.10Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagentDhuha N. Ali AL-ghanamMohammed S. Sheet AL-Enizzi A spectrophotometric method was developed for the determination of methyldopa. The method is based on oxidative coupling reactions with 4,2-dinitrophenylhydrazine as a reagent and in the presence of the oxidizing agent potassium periodate in the acidic medium. The product exhibits maximum absorption at 428 nm, Beer's law-subjective concentration in the range of (1-30) µg/ml, and molar absorbance of 6589,908 liters. mol-1.cm-1. The relative standard deviation was 0.418 percent, the recovery rate was 99.60 percent, and the quantitative limit attained (LOQ) was 0.261 µg/ml. The limit of detection (LOD) was 0.078µg/ml. Following the procedures of continuous changes and molar ratio, the nature of the product created from the reaction was examined, and the ratio was 2:1. (drug compound: reagent) and the value of the stable rate of stability was 8.84 x 1012 liters 2. mol-2, which indicates the good stability of this product. This method was successfully applied to estimate methyldopa in tablet form https://tjpsj.org/index.php/tjps/article/view/10oxidative couplingspectrophotometry2,4- dinitrophenylhydrazineMethyldopa |
| spellingShingle | Dhuha N. Ali AL-ghanam Mohammed S. Sheet AL-Enizzi Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent Tikrit Journal of Pure Science oxidative coupling spectrophotometry 2,4- dinitrophenylhydrazine Methyldopa |
| title | Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent |
| title_full | Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent |
| title_fullStr | Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent |
| title_full_unstemmed | Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent |
| title_short | Spectrophotometric determination of methyldopa by oxidative coupling reactions using 2,4 -dinitrophenylhydrazine reagent |
| title_sort | spectrophotometric determination of methyldopa by oxidative coupling reactions using 2 4 dinitrophenylhydrazine reagent |
| topic | oxidative coupling spectrophotometry 2,4- dinitrophenylhydrazine Methyldopa |
| url | https://tjpsj.org/index.php/tjps/article/view/10 |
| work_keys_str_mv | AT dhuhanalialghanam spectrophotometricdeterminationofmethyldopabyoxidativecouplingreactionsusing24dinitrophenylhydrazinereagent AT mohammedssheetalenizzi spectrophotometricdeterminationofmethyldopabyoxidativecouplingreactionsusing24dinitrophenylhydrazinereagent |