Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM
Eradication of microorganisms present in the root canal system during endodontic therapy is one of the critical factors affecting the final outcome of endodontic treatment. However, even adequate technique of the root canal treatment and use of irrigants according to the established protocol does no...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/11/11/1086 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850217836143706112 |
|---|---|
| author | Radovan Jovanović Jugoslav Ilić Ljiljana Šubarić Zoran Vlahović Sanja Simić Zoran Arsić Milena Radunović Branka Popović |
| author_facet | Radovan Jovanović Jugoslav Ilić Ljiljana Šubarić Zoran Vlahović Sanja Simić Zoran Arsić Milena Radunović Branka Popović |
| author_sort | Radovan Jovanović |
| collection | DOAJ |
| description | Eradication of microorganisms present in the root canal system during endodontic therapy is one of the critical factors affecting the final outcome of endodontic treatment. However, even adequate technique of the root canal treatment and use of irrigants according to the established protocol does not lead to the complete elimination of microorganisms during endodontic treatment. The presence of <i>Enterococcus</i> (<i>E</i>.) <i>faecalis</i> inside the root canal system may be an important factor contributing to the failure of the treatment. Introducing agitation techniques in irrigation, such as passive ultrasonic irrigation (PUI) and the use of the activating instrument XP-endo Finisher (XPF), contribute to a better debridement and disinfection of the root canal. This study was conducted on 42 root canals experimentally inoculated with <i>E. faecalis</i> and exposed to three irrigation protocols. These have included a standard irrigation protocol using a syringe and needle, passive ultrasonic irrigation, and activation of the irrigant using an XPF instrument. The reduction in microorganisms was evaluated using the quantitative polymerase chain reaction in real-time (RT-PCR) as well as via the method of determining the number of colony forming units on nutrient medium (CFUs). The results of this study showed that the use of supplementary irrigation protocols, PUI, and XPF led to a significant decrease in the number of microorganisms inside the root canal of experimental teeth. These findings indicate a significant positive impact of these procedures on the disinfection of the root canal system. |
| format | Article |
| id | doaj-art-e1c62edfc04c474b91cd28928d78aa3e |
| institution | OA Journals |
| issn | 2306-5354 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-e1c62edfc04c474b91cd28928d78aa3e2025-08-20T02:07:58ZengMDPI AGBioengineering2306-53542024-10-011111108610.3390/bioengineering11111086Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEMRadovan Jovanović0Jugoslav Ilić1Ljiljana Šubarić2Zoran Vlahović3Sanja Simić4Zoran Arsić5Milena Radunović6Branka Popović7Dental Clinic, Faculty of Medicine, University of Priština in Kosovska Mitrovica, 38220 Kosovska Mitrovica, SerbiaDepartment of Restorative Odontology and Endodontics, School of Dental Medicine, University of Belgrade, 11000 Beograd, SerbiaDental Clinic, Faculty of Medicine, University of Priština in Kosovska Mitrovica, 38220 Kosovska Mitrovica, SerbiaDental Clinic, Faculty of Medicine, University of Priština in Kosovska Mitrovica, 38220 Kosovska Mitrovica, SerbiaDental Clinic, Faculty of Medicine, University of Priština in Kosovska Mitrovica, 38220 Kosovska Mitrovica, SerbiaDental Clinic, Faculty of Medicine, University of Priština in Kosovska Mitrovica, 38220 Kosovska Mitrovica, SerbiaDepartment of Microbiology and Immunology, School of Dental Medicine, University of Belgrade, 11000 Beograd, SerbiaDepartment of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Beograd, SerbiaEradication of microorganisms present in the root canal system during endodontic therapy is one of the critical factors affecting the final outcome of endodontic treatment. However, even adequate technique of the root canal treatment and use of irrigants according to the established protocol does not lead to the complete elimination of microorganisms during endodontic treatment. The presence of <i>Enterococcus</i> (<i>E</i>.) <i>faecalis</i> inside the root canal system may be an important factor contributing to the failure of the treatment. Introducing agitation techniques in irrigation, such as passive ultrasonic irrigation (PUI) and the use of the activating instrument XP-endo Finisher (XPF), contribute to a better debridement and disinfection of the root canal. This study was conducted on 42 root canals experimentally inoculated with <i>E. faecalis</i> and exposed to three irrigation protocols. These have included a standard irrigation protocol using a syringe and needle, passive ultrasonic irrigation, and activation of the irrigant using an XPF instrument. The reduction in microorganisms was evaluated using the quantitative polymerase chain reaction in real-time (RT-PCR) as well as via the method of determining the number of colony forming units on nutrient medium (CFUs). The results of this study showed that the use of supplementary irrigation protocols, PUI, and XPF led to a significant decrease in the number of microorganisms inside the root canal of experimental teeth. These findings indicate a significant positive impact of these procedures on the disinfection of the root canal system.https://www.mdpi.com/2306-5354/11/11/1086<i>E. faecalis</i>endodonticirrigationinfected canalultrasonic |
| spellingShingle | Radovan Jovanović Jugoslav Ilić Ljiljana Šubarić Zoran Vlahović Sanja Simić Zoran Arsić Milena Radunović Branka Popović Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM Bioengineering <i>E. faecalis</i> endodontic irrigation infected canal ultrasonic |
| title | Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM |
| title_full | Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM |
| title_fullStr | Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM |
| title_full_unstemmed | Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM |
| title_short | Investigating the Antibacterial Ability of Sodium Hypochlorite Solution Activated with PUI and XPF File Against <i>Enterococcus faecalis</i> Using CFU, RT-PCR, and SEM |
| title_sort | investigating the antibacterial ability of sodium hypochlorite solution activated with pui and xpf file against i enterococcus faecalis i using cfu rt pcr and sem |
| topic | <i>E. faecalis</i> endodontic irrigation infected canal ultrasonic |
| url | https://www.mdpi.com/2306-5354/11/11/1086 |
| work_keys_str_mv | AT radovanjovanovic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT jugoslavilic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT ljiljanasubaric investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT zoranvlahovic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT sanjasimic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT zoranarsic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT milenaradunovic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem AT brankapopovic investigatingtheantibacterialabilityofsodiumhypochloritesolutionactivatedwithpuiandxpffileagainstienterococcusfaecalisiusingcfurtpcrandsem |