Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles

In this study, dihydromyricetin (DMY) was used as the core to construct nanoparticles, and DMY was encapsulated by nanoparticles to solve the problems of poor water solubility and poor stability, so as to improve its utilization and biological activity. The anti-solvent precipitation method was used...

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Main Authors: Dawei CHANG, Jiayi CAO, Na REN, Huxuan WANG, Yujiao SUN, Wen SHEN, Sendi SARA
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-06-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050400
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author Dawei CHANG
Jiayi CAO
Na REN
Huxuan WANG
Yujiao SUN
Wen SHEN
Sendi SARA
author_facet Dawei CHANG
Jiayi CAO
Na REN
Huxuan WANG
Yujiao SUN
Wen SHEN
Sendi SARA
author_sort Dawei CHANG
collection DOAJ
description In this study, dihydromyricetin (DMY) was used as the core to construct nanoparticles, and DMY was encapsulated by nanoparticles to solve the problems of poor water solubility and poor stability, so as to improve its utilization and biological activity. The anti-solvent precipitation method was used to develop a Zein-gum Arabic (GA) nano nutrient delivery system with DMY as the core material and Zein as the carrier. The preparation conditions of Zein/GA-DMY nanoparticles were optimized based on particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency. The interactions among Zein, GA, and DMY were examined using Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD), while the system's microscopic morphology was observed through scanning electron microscopy (SEM). The results indicated that when the mass ratio of Zein to GA was 1:2 and the mass ratio of Zein to DMY was 10:1, the nanoparticles achieved a small particle size (191.04 nm), a PDI of 0.164, a zeta potential of −17.70 mV, and an encapsulation efficiency of 76.17%. Hydrogen bonding, electrostatic interactions, and hydrophobic interactions were identified as the primary forces driving nanoparticle formation. DMY was successfully encapsulated in an amorphous state within the Zein/GA-DMY nanoparticles, which were spherical and uniformly distributed. The incorporation of GA enhanced the nanoparticles' stability under varying pH, heat, salt ion concentration, and storage conditions, broadening their potential applications. In vitro antioxidant assays and simulated gastrointestinal digestion experiments demonstrated that DMY retained significant antioxidant activity post-encapsulation and exhibited a slow-release profile. This study provides a theoretical foundation for the application of DMY in functional foods.
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publisher The editorial department of Science and Technology of Food Industry
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series Shipin gongye ke-ji
spelling doaj-art-e1bd3bf4df51468285f70bb6b840ceda2025-08-20T03:46:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-014612304010.13386/j.issn1002-0306.20240504002024050400-12Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of NanoparticlesDawei CHANG0Jiayi CAO1Na REN2Huxuan WANG3Yujiao SUN4Wen SHEN5Sendi SARA6College of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaCollege of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaCollege of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaCollege of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaCollege of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaCollege of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaCollege of Food Science and Engineering, Shaanxi University of Science & Technology, Xi'an 710021, ChinaIn this study, dihydromyricetin (DMY) was used as the core to construct nanoparticles, and DMY was encapsulated by nanoparticles to solve the problems of poor water solubility and poor stability, so as to improve its utilization and biological activity. The anti-solvent precipitation method was used to develop a Zein-gum Arabic (GA) nano nutrient delivery system with DMY as the core material and Zein as the carrier. The preparation conditions of Zein/GA-DMY nanoparticles were optimized based on particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency. The interactions among Zein, GA, and DMY were examined using Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD), while the system's microscopic morphology was observed through scanning electron microscopy (SEM). The results indicated that when the mass ratio of Zein to GA was 1:2 and the mass ratio of Zein to DMY was 10:1, the nanoparticles achieved a small particle size (191.04 nm), a PDI of 0.164, a zeta potential of −17.70 mV, and an encapsulation efficiency of 76.17%. Hydrogen bonding, electrostatic interactions, and hydrophobic interactions were identified as the primary forces driving nanoparticle formation. DMY was successfully encapsulated in an amorphous state within the Zein/GA-DMY nanoparticles, which were spherical and uniformly distributed. The incorporation of GA enhanced the nanoparticles' stability under varying pH, heat, salt ion concentration, and storage conditions, broadening their potential applications. In vitro antioxidant assays and simulated gastrointestinal digestion experiments demonstrated that DMY retained significant antioxidant activity post-encapsulation and exhibited a slow-release profile. This study provides a theoretical foundation for the application of DMY in functional foods.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050400dihydromyricetinzeingum arabicnanoparticlesstabilitybioavailabilitycore-shell structure
spellingShingle Dawei CHANG
Jiayi CAO
Na REN
Huxuan WANG
Yujiao SUN
Wen SHEN
Sendi SARA
Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles
Shipin gongye ke-ji
dihydromyricetin
zein
gum arabic
nanoparticles
stability
bioavailability
core-shell structure
title Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles
title_full Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles
title_fullStr Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles
title_full_unstemmed Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles
title_short Zein-Gum Arabic Loaded with Dihydromyricetin: Preparation and Analysis of Nanoparticles
title_sort zein gum arabic loaded with dihydromyricetin preparation and analysis of nanoparticles
topic dihydromyricetin
zein
gum arabic
nanoparticles
stability
bioavailability
core-shell structure
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050400
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AT jiayicao zeingumarabicloadedwithdihydromyricetinpreparationandanalysisofnanoparticles
AT naren zeingumarabicloadedwithdihydromyricetinpreparationandanalysisofnanoparticles
AT huxuanwang zeingumarabicloadedwithdihydromyricetinpreparationandanalysisofnanoparticles
AT yujiaosun zeingumarabicloadedwithdihydromyricetinpreparationandanalysisofnanoparticles
AT wenshen zeingumarabicloadedwithdihydromyricetinpreparationandanalysisofnanoparticles
AT sendisara zeingumarabicloadedwithdihydromyricetinpreparationandanalysisofnanoparticles