A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry
Bioactive compounds have been shown to promote healthy human development and protect against certain diseases including some cancers. Tropical pumpkins bred for consumption, such as calabaza (Cucurbita moschata), possess these compounds, though concentrations vary considerably between varieties. Bio...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1480964/full |
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| author | Megan E. Kinsman Mary T. Serviss Geoffrey Meru Carlene A. Chase Steven A. Sargent Amarat Simonne Andrew J. MacIntosh |
| author_facet | Megan E. Kinsman Mary T. Serviss Geoffrey Meru Carlene A. Chase Steven A. Sargent Amarat Simonne Andrew J. MacIntosh |
| author_sort | Megan E. Kinsman |
| collection | DOAJ |
| description | Bioactive compounds have been shown to promote healthy human development and protect against certain diseases including some cancers. Tropical pumpkins bred for consumption, such as calabaza (Cucurbita moschata), possess these compounds, though concentrations vary considerably between varieties. Bioactive compound quantification methods can be expensive, complex, and generate considerable hazardous waste. A predictive tool utilizing the relationship between extrinsic properties and tristimulus colorimetry values of calabaza would allow for significantly higher throughput with lower requirements for equipment, training, and laboratory consumables. Therefore, this study examined correlations between flesh color and bioactive compound quantities of several calabaza germplasm lines to assess the utility of a sustainable and portable predictive tool. CIEL* a* b* values of pumpkin flesh were assessed using a colorimeter and compared to bioactive compound concentrations determined analytically. Redness was found to correlate with increased ascorbic acid and total soluble solids content. Yellowness correlated with β-carotene equivalent carotenoids and ascorbic acid content. These results suggest that colorimetry may be a viable pre-screening technique for quantifying bioactive compounds in C. moschata. |
| format | Article |
| id | doaj-art-e1bd141718434a6aad0cca4cc7d6b645 |
| institution | Kabale University |
| issn | 2571-581X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj-art-e1bd141718434a6aad0cca4cc7d6b6452024-11-13T16:08:34ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2024-11-01810.3389/fsufs.2024.14809641480964A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetryMegan E. Kinsman0Mary T. Serviss1Geoffrey Meru2Carlene A. Chase3Steven A. Sargent4Amarat Simonne5Andrew J. MacIntosh6Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesDepartment of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesHorticultural Sciences Department, Tropical Research and Education Center, University of Florida, Homestead, FL, United StatesHorticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United StatesHorticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United StatesDepartment of Family, Youth and Community Sciences, University of Florida, Gainesville, FL, United StatesDepartment of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesBioactive compounds have been shown to promote healthy human development and protect against certain diseases including some cancers. Tropical pumpkins bred for consumption, such as calabaza (Cucurbita moschata), possess these compounds, though concentrations vary considerably between varieties. Bioactive compound quantification methods can be expensive, complex, and generate considerable hazardous waste. A predictive tool utilizing the relationship between extrinsic properties and tristimulus colorimetry values of calabaza would allow for significantly higher throughput with lower requirements for equipment, training, and laboratory consumables. Therefore, this study examined correlations between flesh color and bioactive compound quantities of several calabaza germplasm lines to assess the utility of a sustainable and portable predictive tool. CIEL* a* b* values of pumpkin flesh were assessed using a colorimeter and compared to bioactive compound concentrations determined analytically. Redness was found to correlate with increased ascorbic acid and total soluble solids content. Yellowness correlated with β-carotene equivalent carotenoids and ascorbic acid content. These results suggest that colorimetry may be a viable pre-screening technique for quantifying bioactive compounds in C. moschata.https://www.frontiersin.org/articles/10.3389/fsufs.2024.1480964/fullCucurbita moschatatropical pumpkincolorimetrybioactive compoundsβ-carotenegreen chemistry |
| spellingShingle | Megan E. Kinsman Mary T. Serviss Geoffrey Meru Carlene A. Chase Steven A. Sargent Amarat Simonne Andrew J. MacIntosh A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry Frontiers in Sustainable Food Systems Cucurbita moschata tropical pumpkin colorimetry bioactive compounds β-carotene green chemistry |
| title | A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry |
| title_full | A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry |
| title_fullStr | A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry |
| title_full_unstemmed | A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry |
| title_short | A greener approach to assess bioactive compounds in tropical pumpkin (Cucurbita moschata) using colorimetry |
| title_sort | greener approach to assess bioactive compounds in tropical pumpkin cucurbita moschata using colorimetry |
| topic | Cucurbita moschata tropical pumpkin colorimetry bioactive compounds β-carotene green chemistry |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2024.1480964/full |
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