Cellular reactive oxygen species inhibit MPYS induction of IFNβ.
Many inflammatory diseases, as well as infections, are accompanied by elevation in cellular levels of Reactive Oxygen Species (ROS). Here we report that MPYS, a.k.a. STING, which was recently shown to mediate activation of IFNβ expression during infection, is a ROS sensor. ROS induce intermolecular...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-12-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0015142&type=printable |
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| author | Lei Jin Laurel L Lenz John C Cambier |
| author_facet | Lei Jin Laurel L Lenz John C Cambier |
| author_sort | Lei Jin |
| collection | DOAJ |
| description | Many inflammatory diseases, as well as infections, are accompanied by elevation in cellular levels of Reactive Oxygen Species (ROS). Here we report that MPYS, a.k.a. STING, which was recently shown to mediate activation of IFNβ expression during infection, is a ROS sensor. ROS induce intermolecular disulfide bonds formation in MPYS homodimer and inhibit MPYS IFNβ stimulatory activity. Cys-64, -148, -292, -309 and the potential C₈₈xxC₉₁ redox motif in MPYS are indispensable for IFNβ stimulation and IRF3 activation. Thus, our results identify a novel mechanism for ROS regulation of IFNβ stimulation. |
| format | Article |
| id | doaj-art-e1b9b353ac354eb5aa4402168471d66d |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2010-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e1b9b353ac354eb5aa4402168471d66d2025-08-20T02:01:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-12-01512e1514210.1371/journal.pone.0015142Cellular reactive oxygen species inhibit MPYS induction of IFNβ.Lei JinLaurel L LenzJohn C CambierMany inflammatory diseases, as well as infections, are accompanied by elevation in cellular levels of Reactive Oxygen Species (ROS). Here we report that MPYS, a.k.a. STING, which was recently shown to mediate activation of IFNβ expression during infection, is a ROS sensor. ROS induce intermolecular disulfide bonds formation in MPYS homodimer and inhibit MPYS IFNβ stimulatory activity. Cys-64, -148, -292, -309 and the potential C₈₈xxC₉₁ redox motif in MPYS are indispensable for IFNβ stimulation and IRF3 activation. Thus, our results identify a novel mechanism for ROS regulation of IFNβ stimulation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0015142&type=printable |
| spellingShingle | Lei Jin Laurel L Lenz John C Cambier Cellular reactive oxygen species inhibit MPYS induction of IFNβ. PLoS ONE |
| title | Cellular reactive oxygen species inhibit MPYS induction of IFNβ. |
| title_full | Cellular reactive oxygen species inhibit MPYS induction of IFNβ. |
| title_fullStr | Cellular reactive oxygen species inhibit MPYS induction of IFNβ. |
| title_full_unstemmed | Cellular reactive oxygen species inhibit MPYS induction of IFNβ. |
| title_short | Cellular reactive oxygen species inhibit MPYS induction of IFNβ. |
| title_sort | cellular reactive oxygen species inhibit mpys induction of ifnβ |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0015142&type=printable |
| work_keys_str_mv | AT leijin cellularreactiveoxygenspeciesinhibitmpysinductionofifnb AT laurelllenz cellularreactiveoxygenspeciesinhibitmpysinductionofifnb AT johnccambier cellularreactiveoxygenspeciesinhibitmpysinductionofifnb |