V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i>
<i>Spodoptera exigua</i> is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, <i>S. exigua</i> has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflum...
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2024-10-01
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| author | Xiangjie Liu Minhui Cao Wenjuan Mei Xingliang Wang Yidong Wu |
| author_facet | Xiangjie Liu Minhui Cao Wenjuan Mei Xingliang Wang Yidong Wu |
| author_sort | Xiangjie Liu |
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| description | <i>Spodoptera exigua</i> is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, <i>S. exigua</i> has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflumizone. In this study, we investigated the role of the V1848I mutation in the voltage-gated sodium channel (VGSC) in SCBI resistance and its inheritance patterns in <i>S. exigua</i> through the development and characterization of a near-isogenic resistant strain. The AQ-23 strain of <i>S. exigua</i>, collected in 2023 from Anqing, Anhui province of China, shows 165-fold resistance to indoxacarb compared with the susceptible WH-S strain. A frequency of 44.6% for the V1848I mutation was detected in the <i>SeVGSC</i> of the AQ-23 strain, while no F1845Y mutation was found. Through repeated backcrossing and marker-assisted selection, the V1848I mutation in the AQ-23 strain was introgressed into the susceptible WH-S strain, creating a near-isogenic strain named WH-1848I. This WH-1848I strain exhibits high levels of resistance to indoxacarb (146-fold) and metaflumizone (431-fold) but remains susceptible to broflanilide and spinosad compared with the WH-S strain. Inheritance analysis revealed that SCBI resistance in the WH-1848I strain is autosomal, nonrecessive, and genetically linked to the V1848I mutation. These findings establish a clear link between the V1848I mutation and SCBI resistance in <i>S. exigua</i>, offering valuable insights for developing molecular detection tools and resistance management strategies. |
| format | Article |
| id | doaj-art-e1b5d868ad76460486cf8c0e79f3af64 |
| institution | OA Journals |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-e1b5d868ad76460486cf8c0e79f3af642025-08-20T02:11:13ZengMDPI AGInsects2075-44502024-10-01151077710.3390/insects15100777V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i>Xiangjie Liu0Minhui Cao1Wenjuan Mei2Xingliang Wang3Yidong Wu4Sanya Institute of Nanjing Agricultural University, Sanya 572025, ChinaSanya Institute of Nanjing Agricultural University, Sanya 572025, ChinaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, ChinaSanya Institute of Nanjing Agricultural University, Sanya 572025, ChinaSanya Institute of Nanjing Agricultural University, Sanya 572025, China<i>Spodoptera exigua</i> is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, <i>S. exigua</i> has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflumizone. In this study, we investigated the role of the V1848I mutation in the voltage-gated sodium channel (VGSC) in SCBI resistance and its inheritance patterns in <i>S. exigua</i> through the development and characterization of a near-isogenic resistant strain. The AQ-23 strain of <i>S. exigua</i>, collected in 2023 from Anqing, Anhui province of China, shows 165-fold resistance to indoxacarb compared with the susceptible WH-S strain. A frequency of 44.6% for the V1848I mutation was detected in the <i>SeVGSC</i> of the AQ-23 strain, while no F1845Y mutation was found. Through repeated backcrossing and marker-assisted selection, the V1848I mutation in the AQ-23 strain was introgressed into the susceptible WH-S strain, creating a near-isogenic strain named WH-1848I. This WH-1848I strain exhibits high levels of resistance to indoxacarb (146-fold) and metaflumizone (431-fold) but remains susceptible to broflanilide and spinosad compared with the WH-S strain. Inheritance analysis revealed that SCBI resistance in the WH-1848I strain is autosomal, nonrecessive, and genetically linked to the V1848I mutation. These findings establish a clear link between the V1848I mutation and SCBI resistance in <i>S. exigua</i>, offering valuable insights for developing molecular detection tools and resistance management strategies.https://www.mdpi.com/2075-4450/15/10/777<i>Spodoptera exigua</i>indoxacarbmetaflumizonesodium channelresistance |
| spellingShingle | Xiangjie Liu Minhui Cao Wenjuan Mei Xingliang Wang Yidong Wu V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i> Insects <i>Spodoptera exigua</i> indoxacarb metaflumizone sodium channel resistance |
| title | V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i> |
| title_full | V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i> |
| title_fullStr | V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i> |
| title_full_unstemmed | V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i> |
| title_short | V1848I Mutation in the Voltage-Gated Sodium Channel Confers High-Level Resistance to Indoxacarb and Metaflumizone in <i>Spodoptera exigua</i> |
| title_sort | v1848i mutation in the voltage gated sodium channel confers high level resistance to indoxacarb and metaflumizone in i spodoptera exigua i |
| topic | <i>Spodoptera exigua</i> indoxacarb metaflumizone sodium channel resistance |
| url | https://www.mdpi.com/2075-4450/15/10/777 |
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